ABSTRACTS – ORAL PRESENTATIONS - AMCA, spol. s r.o.

In summary, we described different subpopulations of CSCs in a panel of prostate
epithelial cell lines and determined EMT status of those subpopulations.
Acknowledgements
This work was supported by grants IGA MZD NT13573-4/2012, AV ČR M200041203,
and by project FNUSA-ICRC (no. CZ.1.05/1.1.00/02.0123) from the European Regional
Development Fund. Institutional support was provided by the Academy of Sciences of
the Czech Republic.
References
Kong, D., S. Banerjee, et al. (2010). “Epithelial to Mesenchymal Transition Is Mechanistically
Linked with Stem Cell Signatures in Prostate Cancer Cells.” PLoS ONE 5(8): e12445.
Mani, S. A., W. Guo, et al. (2008). “The Epithelial-Mesenchymal Transition Generates
Cells with Properties of Stem Cells.” Cell 133(4): 704-715.
P26. POTENTIAL INHIBITORS OF GLUCOSYLCERAMIDE SYNTHASE AND THEIR EFFECT
ON CELL VIABILITY AND CALCIUM TRANSPORT
Boris Lakatoš* 1 , Katarína Turáková 1 , Daniela Moravčíková 2 , Andrej Duriš 2 , Dušan Berkeš 2
1
Department of Biochemistry and Microbiology, Faculty of Chemical and Food
Technology, Slovak University of Technology, Bratislava, Slovak Republic;
2
Department of Organic Chemistry, Faculty of Chemical and Food Technology, Slovak
University of Technology, Bratislava, Slovak Republic;
*boris.lakatos@stuba.sk
Glucosylceramide (GlcCer) is a fundamental glycosylated lipid found in organisms ranging
from mammals to fungi. It is composed of a hydrophilic β-linked glucose and a hydrophobic
ceramide. Mammalian GlcCer mainly contains sphingosine (d18:1), a sphingoid base
that has one double bond at the C4 position in a trans conformation, and N linked C16–
C24 fatty acids. GlcCer is the precursor of hundreds of different glycosphingolipids. This
cerebroside is synthesized from uridine diphosphate-glucose and ceramide by a GlcCer
synthase (UDP-glucose:ceramide glucosyltransferase; UGCG, GlcT-1, CGT, or GCS, EC
2.4.1.80). GlcCer-based sphingolipids have been identified as important mediators of a
variety of cellular functions, including proliferation, differentiation, development, and
cell-cell recognition (1). There is several pathological situations which are related with
disequilibrium of sphingolipids in cells, e.g. cancer, diabetes, neurological diseases (2).
Aim of this work was to study effects of several newly synthesized derivatives of (±)-threo-
1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP), which is known inhibitor
of GlcCer synthase, on activity of this enzyme, cell viability and calcium transport. As
a model cells we used thymocytes isolated from thymus of 3-7 weeks old mice (ICR
strain). Changes of calcium transport were measured using Ca 2+ sensitive probe Fluo-3
on spectrofluorometer FluoroMax-4. The activity of GlcCer synthase was assessed from
lipid extracts of cells cultivated with derivatives by thin layer chromatography using NBD-
C12-ceramide. HPTLC plates were analyzed using phosphoimager Typhoon 9210 and cell
viability was determined by flow cytometry and direct cell counting.
118 Analytical Cytometry VII