ABSTRACTS â ORAL PRESENTATIONS - AMCA, spol. s r.o.
ABSTRACTS â ORAL PRESENTATIONS - AMCA, spol. s r.o.
ABSTRACTS â ORAL PRESENTATIONS - AMCA, spol. s r.o.
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These results indicate that the promising in vitro effects of pterostilbene on ROS/RNS<br />
production operate by different mechanisms and its beneficial activity in experimental<br />
arthritis may be attributed to regulation of neutrophil numbers.<br />
Acknowledgement<br />
Supported by the grants APVV-0052-10 and 0315-07, VEGA-2/0010/13 and<br />
CZ.1.07/2.3.00/30.0030.<br />
References<br />
Edwards, S. W., and Hallett, M. B.: Seeing the wood for the trees: the forgotten role of<br />
neutrophils in rheumatoid arthritis. - Immunology Today 18: 320-324, 1997.<br />
Fox, S., Leitch, A. E., Duffin, R., Haslett, C., Rossi, A. G.: Neutrophil apoptosis: relevance<br />
to the innate immune response and inflammatory disease. – Journal of Innate Immunity<br />
2: 216-227, 2010.<br />
Witko-Sarsat, V., Rieu, P., Descamps-Latscha, B., Lesavre, P., Halbwachs-Mecarelli,<br />
L. Neutrophils: molecules, functions and pathophysiological aspects. - Laboratory<br />
Investigation 80: 617-653, 2000.<br />
P24. Β-ARRESTIN PROMOTES WNT-INDUCED LRP6 PHOSPHORYLATION VIA<br />
INCREASED MEMBRANE RECRUITMENT OF AMER1<br />
Vítězslav Kříž 1,2* , Vendula Pospíchalová 1*# , Jan Mašek 1,6 , Michaela Brita Christina<br />
Kilander 4 , Josef Slavík 5 , Kristina Tanneberger 3 , Gunnar Schulte 1,4 , Miroslav Machala 5 ,<br />
Alois Kozubík 1,2 , Juergen Behrens 3 and Vítězslav Bryja 1,2<br />
1<br />
Faculty of Science, Institute of Experimental Biology, Masaryk University, Brno, Czech<br />
Republic<br />
2<br />
Department of Cytokinetics, Institute of Biophysics, Academy of Science of the Czech<br />
Republic, Brno, Czech Republic<br />
3<br />
Nikolaus-Fiebiger-Center, University Erlangen-Nürnberg, Erlangen, Germany<br />
4<br />
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden<br />
5<br />
Department of Toxicology, Pharmacology and Immunotherapy, Veterinary Research<br />
Institute, Brno, Czech Republic<br />
6<br />
Institute of Molecular Genetics, Academy of Science of the Czech Republic, Prague,<br />
Czech Republic<br />
*equal contribution<br />
#<br />
contact email: pospich@sci.muni.cz<br />
β-arrestin is a scaffold protein, which regulates signal transduction by seven<br />
transmembrane spanning receptors. Among other functions it is also critically required<br />
for Wnt/β-catenin signal transduction (for review see Schulte G, et al., 2010). In the<br />
present study we provide for the first time a mechanistic basis for the β-arrestin function<br />
in Wnt/β-catenin signaling. We demonstrate that β-arrestin is required for efficient<br />
Wnt3a-induced Lrp6 phosphorylation, a key event in downstream signaling (Pan W,<br />
et al., 2008). β-arrestin regulates Lrp6 phosphorylation via a novel interaction with<br />
phosphatidylinositol (4,5) bisphosphate (PtdIns(4,5)P 2<br />
)-binding protein Amer1/WTX/<br />
Analytical Cytometry VII 115
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