ABSTRACTS – ORAL PRESENTATIONS - AMCA, spol. s r.o.

aberrant expression, localization and phosphorylation of connexin43 in vitro. They are
also characterized by a loss of contact inhibition of growth and capability of anchorageindependent
growth.
Chemopreventive and anticancer effects of phytochemicals tested were evaluated on
three different levels (endpoints). Restoration of oncogene-inhibited gap junctional
intercellular communication (GJIC), which represents a key cellular event linked to
tumor promotion and chemoprevention, was investigated in WB-ras cell line using
scrape loading/dye transfer assay. Selective antiproliferative and/or cytotoxic effects of
phytochemicals towards tumorigenic WB-ras cells were assessed by neutral red uptake
assay and compared with phytochemical effects in normal non-tumorigenic WB-F344
cell line. In addition to that, alterations of cell cycle induced by phytochemial treatment
were also studied in both cell lines using propidium iodide staining and flow cytometry
measurement.
The results of conducted experiments suggested that tested phytochemicals were able
to selectively affect evaluated parameters in tumorigenic WB-ras cells, indicating their
chemopreventive and anticancer activity as well as possible alterations of ras-dependent
signaling. The employed in vitro models/methods were thus demonstrated to be a
valuable and effective tool for future phytochemical and dietary compound screening
for cancer chemopreventive and anticancer activity and for further characterization of
their mechanisms of action.
Acknowledgements
This work was supported by a research grant of the Ministry of Education, Youth and Sports
LH12034 (CHEMOPREV) - Novel in vitro approach for identification of chemopreventive
effects and mechanisms of phytochemicals (LH KONTAKT II funding programme).
P20. IMMUNOMODULATORY PROPERTIES OF CANDIDA GLABRATA CELL WALL
MANNAN
Lucia Paulovičová 1 , Ema Paulovičová 1 , Eva Pericolini 2 , Elena Gabrielli 2 , Anna Vecchiarelli 2
1
Center of Excellence Glycomed, Department of Immunochemistry of Glycoconjugates,
Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovak Republic;
chemluli@savba.sk
2
Microbiology Section, Department of Experimental Medicine and Biochemical Sciences,
University of Perugia, Perugia, Italy
The yeasts Candida glabrata is a human commensal fungus that resides on the skin,
mucosa surfaces and gastrointestinal tract of healthy individuals. Although C. glabrata is
not pathogenic under normal host conditions, it can cause infections when host defence
is compromised. In Slovakia C. glabrata was the second abundant human pathogen
according to the frequencies in clinical isolates from upper (16.3%), low respiratory
tract (24.5%) and gynaecological swabs (6%) following C. albicans (Mycology Labs.,
HPL ,Slovakia). This fungus is associated with a wide spectrum of diseases in humans,
ranging from acute superficial infections of skin and mucosal membranes due to impaired
110 Analytical Cytometry VII