ABSTRACTS – ORAL PRESENTATIONS - AMCA, spol. s r.o.

In our work, we focus on exploring the other factors involved in hypoxia-induced
chemoresistance, both dependent and independent on HIF-1α. We have supressed the
expression of HIF-1α in UKF-NB-4 cell line by shRNA gene silencing. In this cell line, we
observed lower hypoxia induced chemoresistance to cisplatin, but not its absolutely
withdrawal, which is consistent with our previous results, when we used small-molecule
HIF-1α inhibotors and siRNA gene silencing. We started to work with hypoxia-conditioned
media and observed that the chemoresistance is still present also in cells incubated with
media coming from the HIF-1α knockdown cell line. We also focused on a research on
V-ATPase and its impact on chemoresistance.
The research results will contribute to the understanding of the biological properties of
neuroectodermal tumors, especially the hypoxia-induced resistance to cytostatics.
Acknowledgements
This work is supported by GA UK 620612.
References
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Schnitzer, S. E., Schmid, T., Zhou, J., et al.: Hypoxia and HIF-1alpha protect A549 cells
from drug-induced apoptosis. - Cell Death Differ. 2006;13(9):1611-3.
Semenza, G. L.: HIF-1: upstream and downstream of cancer metabolism. Curr Opin
Genet Dev. 2010 Feb; 20(1):51-6. Epub 2009 Nov 26.
Yee Koh, M., et al.: HIF-1 regulation: not so easy come, easy go. - Trends Biochem Sci.
2008 Nov; 33(11):526-34. Epub 2008 Sep 21.
P19. EVALUATION OF CHEMOPREVENTIVE ACTIVITY OF PHYTOCHEMICALS USING
NORMAL AND TUMORIGENIC RAT LIVER EPITHELIAL CELLS
Premysl Mikula 1 , Zuzana Lencesova 1,2 , James Trosko 3 , Brad Upham 3 , Pavel Babica 1,2
1
Institute of Botany of the ASCR, Department of Experimental Phycology and
Ecotoxicology, Lidicka 25/27, 602 00 Brno, Czech Republic; premysl.mikula@centrum.cz
2
Masaryk University, RECETOX – Research Centre for Toxic Compounds in the Environment,
Kamenice 753/5, 625 00 Brno, Czech Republic
3
Michigan State University, Department of Pediatrics and Human Development, East
Lansing, MI 48824, USA
Cancer chemopreventive and anticancer potential of selected phytochemicals (e.g.
metformin, silibinin and quercetin) was evaluated in WB-F344 rat liver epithelial cells as
well as in WB-ras cell line derived from WB-F344 cells by stable transduction with H-ras
oncogene.
While WB-F344 cell line represents normal non-tumorigenic diploid cells, expressing
gap-junctional protein connexin43 and communicating via gap-junctional channels,
which may differentiate into functional hepatocytes or cardiomyocytes upon injection
into a syngenic Fischer 344 rat, WB-ras cells are highly tumorigenic in vivo and show
Analytical Cytometry VII 109