ABSTRACTS – ORAL PRESENTATIONS - AMCA, spol. s r.o.

that the interaction of MPO with glycocalyx GAGs can lead to collapse of the glycocalyx
structure. In order to get an insight into the mechanism how MPO may affect this, MPO
mediated modulation of viscosity of GAGs solution as a degree of modulation of GAG
three dimensional structure was determined. Data show a remarkable MPO mediated
increase of the viscosity of two model GAGs hyaluronate and chondroitin sulphate.
Further, MPO mediated decrease of GAG intrinsic negative charge at physiological
conditions was evaluated. Different experimental settings were tested to stabilize GAGs
in three dimensional structures which allowed the determination of the charge change
employing dyes alcian blue X8 or direct red. Results suggest that staining with both dyes
corresponds in a linear manner to the amount of embedded GAGs and that cationic
proteins decrease the overall charge of embedded GAGs. Interestingly, similar effect was
observed in vivo in mouse after application of alcian blue into the carotid artery and
consequent loss of staining after application of MPO.
These findings extend the knowledge of MPO inflammatory properties in vascular
inflammation.
Acknowledgements
This work was supported by grants from the Czech Science Foundation No. P305/12/
J038 and from DFG BA1870/9-1; KL 2516/1-1 and the European Social Fund - Project
OganoNET (CZ.1.07/2.4.00/31.0245) and HistoPARK (CZ.1.07/2.3.00/20.0185). LK was
supported by the European Regional Development Fund - Project FNUSA-ICRC (No.
CZ.1.05/1.1.00/02.0123).
P17. ROSIGLITAZONE ACTS AS AN EFFICIENT MODULATOR OF LA-12-INDUCED
CYTOTOXIC AND CYTOSTATIC RESPONSE OF COLON CANCER CELLS
Jarmila Lauková 1,2,3 , Iva Jelínková 1,2 , Jiřina Hofmanová 1,2 , Nicol Straková 1,6 , Petr Sova 4 , Jiří
Ehrmann 5 , Zdeněk Kolář 5 , Alois Kozubík 1,2 and Alena Hyršlová Vaculová 1,3
1
Department of Cytokinetics, Institute of Biophysics, Academy of Sciences of the Czech
Republic, v.v..i., Brno, Czech Republic; laukova@ibp.cz, vaculova@ibp.cz
2
Institute of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech
Republic
3
Center of Biomolecular and Cellular Engineering, International Clinical Research Center,
St. Anne´s University Hospital Brno, Czech Republic
4
Platinum Pharmaceuticals, a.s., Brno, Czech Republic
5
Department of Clinical and Molecular Pathology, Faculty of Medicine and Dentistry,
Palacky University Olomouc, Czech Republic
Rosiglitazone, a synthetic ligand of peroxisome proliferator-activated receptor gamma
(PPARgamma) can suppress proliferation and stimulate death of colon cancer cells.
In addition, it may also act as an agent sensitizing the cancer cells to the action of
selected chemotherapeutic drugs, which suggests its potential application in antitumor
therapy. Our results showed that pretreatment of HCT116 human colon cancer cells
with rosiglitazone resulted in enhancement of apoptosis induced by platinum (IV)
Analytical Cytometry VII 107