ABSTRACTS – ORAL PRESENTATIONS - AMCA, spol. s r.o.

was analyzed. We found out that, in the site of infection, B-1a cells are activated very
early after infection (within 12 hours) and in this time B cells relatively broad spectrum of
cytokines. Thus, considering the protective efficacy of circulating antibodies, production
of cytokines, and potent antigen-presenting function of B cells, B cell-mediated, as well
as T cell-mediated immunity plays an equivalent role in control of F. tularensis infection
in mice.
Acknowledgement
The authors are supported by the grant from Grant Agency of Czech Republic No.
P302/11/1631
P15. SENSITIZATION OF CELLS TOWARDS ANTICANCER TREATMENT BY INHIBITORS OF
SUCCINATE DEHYDROGENASE
Björn Kruspig 1 , Belma Skender 2,3 , Boris Zhivotovsky 1 , and Vladimir Gogvadze 1
1
Institute of Environmental Medicine, Division of Toxicology, Karolinska Institutet, Box
210, Stockholm, SE-171 77 Sweden
2
Department of Cytokinetics, Institute of Biophysics, Academy of Sciences of the Czech
Republic, v.v.i., Královopolská 135, 612 65 Brno, Czech Republic
3
Department of Animal Physiology and Immunology, Institute of Experimental Biology,
Faculty of Science, Masaryk University, Terezy Novákové 64, 621 00 Brno, Czech
Republic
Cancer and apoptosis are regarded as antagonistic processes. Apoptosis may be
involved in spontaneous regression of tumors, whereas defects in apoptotic pathways
may contribute to tumor progression and resistance to treatment. Therefore, the
stimulation of cell death is the most widely used tools in cancer therapy. Considering
mitochondria as key participants in various forms of cell death steps directed to
mitochondrial destabilization, permeabilization of the outer mitochondrial membrane
(OMM) and release of pro-apoptotic proteins represent a promising strategy in fighting
cancer. Mitochondria are multifunctional organelles, playing different vital roles in
cell metabolism, survival and death. One of their essential physiological functions is
the generation of ATP for metabolic demands through the oxidative phosphorylation
(OXPHOS) pathway. They are also known as a major site of reactive oxygen species (ROS)
production, which participates in different signaling pathways, but might cause cell
damage if produced excessively. In addition, mitochondria are involved in intracellular
Ca 2+ homeostasis regulation. All these functions of mitochondria are essential for cell
survival and also play an important role in cell death stimulation, thus making these
organelles an attractive target for cancer therapy.
In the present work we analyzed possible consequences of combined treatment of
tumor cells with inhibitors of succinate dehydrogenase (complex II of the mitochondrial
respiratory chain) – thenoyltrifluoroacetone (TTFA) and methyl-malonate (MM) – in
combination with the conventionally used anticancer drug cisplatin.
Incubation of neuroblastoma Tet21N and SK-N-BE(2) cells with 5 µg/ml cisplatin for 24
Analytical Cytometry VII 105