ABSTRACTS – ORAL PRESENTATIONS - AMCA, spol. s r.o.

models. As manifested by compromised fertilization parameters, some transgenic boars
of miniature pig line bearing mutated N-terminal part of human huntingtin produce
low quality semen too. Functional spermatozoa mitochondrion plays a substantial role
in oocyte fertilization, therefore the impact of mHtt expression on semen quality and
spermatozoa parameters had been determined using flowcytometry approach.
The mitochondrial mass was estimated by nonyl Acridine Orange (NAO) staining of
cardiolipin. NAO signals do not differ between transgenic (Tg) and wild type (Wt)
spermatozoa. Although defective in motility Tg spermatozoa displayed mitochondrial
membrane potential similar to Wt as assessed by JC-1 staining.
Both Tg and Wt spermatozoa produce ROS spontaneously as determined by MitoSOX TM
Red (MSR) reagent. While there is no difference in maximal levels of MSR signal measured
between Wt and Tg animals, higher ROS production is indicated by sooner development
of detectable MSR signal during Tg spermatozoa staining procedure compared to Wt
spermatozoa.
Acknowledgement
This work was supported by the CHDI Foundation (ID 1035, A5378), The Technical
Agency of the Czech Republic (TA01011466), RVO 67985904, and European Regional
Development Fund CZ.1.05/2.1.00/03.0124.
P10. THE CELL DEATH INDUCED BY TAXANES IN PANCREATIC CANCER CELL LINES
Katerina Kloudova 1,2 , Marie Ehrlichova 1 , Radka Vaclavikova 1 , Veronika Brynychova 1,2 ,
Martin Oliverius 3 , Eva Honsova 3 , Matej Kocik 3 , Iwao Ojima 4 and Pavel Soucek 1
1
Laboratories of Toxicogenomics, National Institute of Public Health in Prague, Czech
Republic; katerina.kloudova@szu.cz
2
3 rd Faculty of Medicine, Charles University in Prague, Czech Republic;
3
Institute for Clinical and Experimental Medicine, Prague, Czech Republic;
4
Institute of Chemical Biology & Drug Discovery and Department of Chemistry, State
University of New York at Stony Brook, New York, USA.
Pancreatic carcinoma is one of the most aggressive tumours with high mortality
worldwide (Jemal et al., 2007). Gemcitabine is primary cytotoxic agent used in the
therapy of pancreatic carcinoma. Unfortunately, treatment with gemcitabine has so far
proved ineffective (Hildago, 2010). Combined therapy together with taxane presents
effort to increase of antitumor effect of gemcitabine (Frese et al., 2012). Taxanes are
successfully used in therapy of various human cancers, mainly of breast and ovarian
carcinomas. However, inherited or acquired resistance of tumour cells to taxanes
(paclitaxel, docetaxel) presents one of the major obstacles in successful therapy. Drug
resistance is a multifactorial process that may be related to drug transport, metabolism
or alterations in apoptosis induction by e.g. taxanes. Certain novel taxanes (i.e. taxanes
of second generation or fluorinated taxanes) possess extremely high potency against
drug-resistant cancer cells expressing the multidrug resistance (MDR) phenotype (Ojima
et al., 1996; Ehrlichova et al., 2012) The aim of our study was to investigate the effect
98 Analytical Cytometry VII