ABSTRACTS – ORAL PRESENTATIONS - AMCA, spol. s r.o.

carcinoma cells. Since proadifen is a P450 monooxygenase inhibitor, the possibility that
cisplatin effect is influenced by drug-metabolizing enzymes could not be excluded. We
hypothesize that proadifen may be used as a strategy for the sensitization of resistant
ovarian carcinomas to cisplatin treatment. However, future studies will be needed to
establish the impact of proadifen on cisplatin action in vivo.
Acknowledgements
This work was supported by the Slovak Research and Development Agency under
contract Nos. APVV-0040-10 and VVCE-0001-07 and the Scientific Grant Agency of the
Ministry of Education of the Slovak Republic under contract No. VEGA 1/0626/11.
References
Hoferová, Z., Souček, K., Hofmanová, J., Hofer, M., Chramostová, K., Fedoročko, P., and
Kozubík, A.: In vitro proliferation of fibrosarcoma cells depends on intact functions of
lipoxygenases and cytochrome P-450-monooxygenase. - Cancer Investigation 22: 234-
247, 2004.
Jendželovský, R., Kovaľ, J., Mikeš, J., Papčová, Z., Plšíková, J., and Fedoročko, P.: Inhibition
of GSK-3β reverses the pro-apoptotic effect of proadifen (SKF-525A) in HT-29 colon
adenocarcinoma cells. - Toxicology in Vitro 26: 775-782, 2012.
Jendželovský, R., Mikeš, J., Kovaľ, J., Souček, K., Procházková, J., Kello, M., Sačková,
V., Hofmanová, J., Kozubík, A., and Fedoročko, P.: Drug efflux transporters, MRP1
and BCRP, affect the outcome of hypericin-mediated photodynamic therapy in HT-29
adenocarcinoma cells. - Photochemical & Photobiological Sciences 8: 1716-1723, 2009.
Materna, V., Liedert, V., Thomale, J., and Lage, H.: Protection of platinum-DNA adduct
formation and reversal of cisplatin resistance by anti-MRP2 hammerhead ribozymes in
human cancer cells. - International Journal of Cancer 115: 393-402, 2005.
Wang, H., Li, X., Chen, T., Wang, W., Liu, Q., Li, H., Yi, J., and Wang, J.: Mechanisms of
verapamil-enhanced chemosensitivity of gallbladder cancer cells to platinum drugs:
glutathione reduction and MRP1 downregulation. - Oncology Reports 29: 676-684, 2013.
P9. MITOCHONDRIAL STATUS OF MHTT MINIATURE PIG SPERMATOZOA DETERMINED
BY FLOWCYTOMETRY
Jiri Klima, Ivona Valekova, Monika Macakova, Antonin Pavlok, Jan Motlik
Institute of Animal Physiology and Genetics, Academy of Sciences of Czech Republic,
Libechov, Czech Republic; klima@iapg.cas.cz
Huntington´s disease (HD) is caused by CAG repeat expansion in huntingtin gene. Yet
uncharacterized „gain of function“ of mutant huntingtin (mHtt) is responsible for this
devastating disorder affecting not only particular neural cell populations of central neural
system but also other cells, tissues and organs. MHtt affects various cellular processes
among them are mitochondrial biogenesis, mitophagy and metabolism. Moreover,
mitochondria are involved in mHtt driven adverse effects like excitotoxicity or elevated
formation of reactive oxygen species (ROS).
Reproductive defects have also been documented both in HD patients and small animal
Analytical Cytometry VII 97