ABSTRACTS – ORAL PRESENTATIONS - AMCA, spol. s r.o.

kappaB. Beside that, we found OANO 2
-dependent inhibition of NADPH oxidase, iNOS,
CD36, and TLR2/4 expression in endotoxin-stimulated RAW 264.7 cells.
We can conclude that OANO 2
is able to significantly reduce the oxidative and nitrative
stress, generated within the macrophage-dependent innate immune responses, via the
regulation of crucial intracellular signalling pathways connected with MAPK and NFkappaB
activation.
Acknowledgements: This work was supported by the grant of GAČR 13-40824P.
References:
Baker, P. R., Schopfer, F. J., O’Donnell, V. B., and Freeman, B. A.: Convergence of nitric
oxide and lipid signaling: anti-inflammatory nitro-fatty acids, Free Radic Biol Med 46,
989-1003, 2009.
Rudolph, T. K., Rudolph, V., Edreira, M. M., Cole, M. P., Bonacci, G., Schopfer, F. J.,
Woodcock, S. R., Franek, A., Pekarova, M., Khoo, N. K., Hasty, A. H., Baldus, S., and
Freeman, B. A.: Nitro-fatty acids reduce atherosclerosis in apolipoprotein E-deficient
mice, Arterioscler Thromb Vasc Biol 30, 938-945, 2010.
Trostchansky, A., and Rubbo, H.: Nitrated fatty acids: mechanisms of formation, chemical
characterization, and biological properties, Free Radic Biol Med 44, 1887-1896, 2008.
P2. THE ROLE OF AUTOPHAGY IN MODULATION OF 5-FU-INDUCED DEATH RESPONSE
IN COLON CANCER CELLS WITH DIFFERENT P53 STATUS
Barbora Bujokova 1,2 , Iva Jelinkova 1,2 , Birce Akpinar 3 , Alois Kozubik 1,2 , Boris Zhivotovsky 3 ,
Magnus Olsson 3 , Alena Hyrslova Vaculova 1
1
Department of Cytokinetics, Institute of Biophysics, Academy of Sciences of the Czech
Republic, v.v.i., Brno, Czech Republic
2
Department of Animal Physiology and Immunology, Faculty of Science, Masaryk
University, Brno, Czech Republic
3
Division of Toxicology, Institute of Environmental Medicine, Karolinska Institutet,
Stockholm, Sweden
Autophagy is a cellular catabolic pathway by which macromolecules and organelles
are sequestered in autophagosomes and delivered to the lysosomes for degradation.
Autophagy may serve both, to promote cell survival or execute cellular demise. Autophagy
plays an important role in carcinogenesis, and can also be induced by chemotherapeutic
drugs in various tumor types. The role of autophagy in cancer therapy still needs to
be clarified, as it has been shown either counteract or mediate the cytotoxic effects of
some anticancer agents. Thus, selective modulation of autophagy may be considered as
a novel and effective approach for enhancing the efficacy of existing anticancer therapy.
5-Fluorouracil (5-FU) is a well-known chemotherapeutic drug used in the treatment of
patients with colorectal cancer. Intracellular metabolites of 5-FU can exert their cytotoxic
effects via inhibition of thymidylate synthase, or through incorporation into DNA and
RNA, events that finally induce cell cycle arrest or apoptosis. In addition, autophagy
has also been reported as an important mechanism in the cellular response to 5-FU.
88 Analytical Cytometry VII