ABSTRACTS – ORAL PRESENTATIONS - AMCA, spol. s r.o.

(Broad Institute, Boston MA). Ag-activated cells spread
on fibronectin whereas upon PGE 2
stimulation the
cells created F-Actin rich protrusions. Cell area was
determined and normalized to non-activated cells (D).
At least 300 cells were evaluated in each condition.
Means ±SD were calculated from duplicates in one
representative experiment. Bars 20 mm.
ABSTRACTS – POSTERS
P1. NITRO-OLEIC ACID ATTENUATES THE INNATE IMMUNE RESPONSES OF
MACROPHAGES STIMULATED WITH BACTERIAL ENDOTOXIN
Gabriela Ambrozova 1,2 , Lukas Kubala 1,2 , Tanja K. Rudolph 3 , Antonin Lojek 1 , Thorben
Ravekes 3 , Michaela Pekarova 1
1
Institute of Biophysics AS CR, Brno, Czech Republic; ambrozova@ibp.cz
2
ICRC/FNUSA, Brno, Czech Republic;
3
Hamburg University Heart Center, Hamburg, Germany
Nitrated fatty acids (NO 2
-FAs) represent a novel group of pluripotent signalling molecules,
endogenously generated as an adaptive response of organism to oxidative stress (Baker
et al., 2009). NO 2
-FAs were shown to exert significant anti-inflammatory signalling action
in immune and vascular models and so are hypothesized as protective compounds that
can effectively down regulate ineligible activation of innate immune cells, particularly
professional phagocytes (Trostchansky and Rubbo, 2008). Protective effects of NO 2
-FAs
on severe diseases including pulmonary hypertension and atherosclerosis have been
described (Rudolph et al., 2010). Nevertheless, detailed mechanisms of their action in
regulation of innate immune responses are not completely understood. Therefore, we
investigated the role and mechanism of action of nitro-oleic acid (OANO 2
) in endotoxinstimulated
RAW 264.7 macrophages.
Our results showed that OANO 2
prevented the endotoxin-induced activation of
macrophages in a dose-dependent manner. It was able to down-regulate phagocytic
capability, production of reactive oxygen and nitrogen species as well as inflammatory
cytokines. Importantly, changes in the production of inflammatory mediators were
associated with reduction of endotoxin-induced MAPK activation and expression of NF-
Analytical Cytometry VII 87