ABSTRACTS – ORAL PRESENTATIONS - AMCA, spol. s r.o.

cells (LSC) are present in CD45+34+38-26+ subpopulation only. Loss of expression of
CD26 on Ph - cells from patients with pattern 2 appears to be specific for after-therapy
phase of the disease. On the other hand, not all CD45 + 34 + 38 - 26 + cells were found Ph
positive.
Our findings confirm that CD26 antigen is useful for discrimination between Ph + and
Ph - cells in exact disease phase and/or pattern-dependent only. However, considering
easy identification of chronic myeloid stem cells by Ph positivity in CD45 + 34 + 38 - 26 - or
CD45 + 34 + 38 - 26 + populations, it is obvious that precise separation LSC from HSC in target
populations using CD26 is not possible in all settings. Despite these facts, this approach
opens up a new possibilities for CML study.
Acknowledgements
Supported by MH CZ - DRO (FNBr, 65269705)
References
Gerber JM, Gucwa JL, Esopi D, Gurel M, Haffner MC, Vala M, Nelson WG, Jones RJ,
Yegnasubramanian S.: Genome-wide comparison of the transcriptomes of highly
enriched normal and chronic myeloid leukemia stem and progenitor cell populations.
Oncotarget. 2013
Janssen JJ, Deenik W, Smolders KG, van Kuijk BJ, Pouwels W, Kelder A, Cornelissen JJ,
Schuurhuis GJ, Ossenkoppele GJ.: Residual normal stem cells can be detected in newly
diagnosed chronic myeloid leukemia patients by a new flow cytometric approach and
predict for optimal response to imatinib. Leukemia 2012
64. EPITHELIAL-TO-MESENCHYMAL TRANSITION IN SUBPOPULATION OF MOUSE
PROSTATE STEM CELLS AND CANCER STEM-LIKE CELLS
Zuzana Pernicová 1,2 , Eva Slabáková 1,2 , Radek Fedr 1 , Maximilian Marhold 3 , Erwin
Tomasich 3 , Peter Horak 3 , Alois Kozubík 1,4 , Karel Souček 1,2
1
Department of Cytokinetics, Institute of Biophysics, Academy of Sciences of the Czech
Republic, Brno, Czech Republic; E-mail: pernicova@ibp.cz
2
Center of Biomolecular and Cellular Engineering, International Clinical Research Center,
St. Anne‘s University Hospital Brno, Brno, Czech Republic
3
Department of Internal Medicine I – Oncology, Comprehensive Cancer Center, Medical
University of Vienna, Wien, Austria
4
Department of Experimental Biology, Faculty of Sciences, Masaryk University, Brno,
Czech Republic
Epithelial-to-mesenchymal transition (EMT) is an important process during
embryogenesis, which may be re-activated in several pathophysiological processes
including tumor progression. In several models, normal cells or cancer cells undergoing
EMT display properties and characteristics of stem cells (Kong et al., 2011; Mani et al.,
2008). In our work, we aimed to identify EMT transcription factors with altered expression
in subpopulation of mouse prostate stem cells or cancer stem cells, respectively, to prove
involvement of EMT in biology of stem cells also in mouse prostate. There are several
Analytical Cytometry VII 81