ABSTRACTS – ORAL PRESENTATIONS - AMCA, spol. s r.o.

Furthermore, they may unravel targets to interfere with in order to affect tumor stromal
interaction and/or inhibit tumor growth. Moreover, counteracting inhibitory effects
may enable to grow tumors and provide novel in vivo models as well as combination of
differentiated tumor associated fibroblasts with tumor cells may produce more relevant
tumor models for evaluation of tumor behavior and responses in vivo.
Acknowledgements
This work was supported by the Slovak Research and Development Agency under the
contract No.APVV-0230-11, VEGA grants 2/0088/11 and 2/0171/13. The experiments on
the IncuCyte ZOOM were enabled with the kind help and the financial support from
the Cancer Research Foundation.
1. Kucerova L, Matuskova M, Hlubinova K, Altanerova V, Altaner C: Tumor cell behaviour
modulation by mesenchymal stromal cells. Mol Cancer 2010, 9:129.
2. Kucerova L, Kovacovicova M, Polak S, Bohac M, Fedeles J, Palencar D, Matuskova M:
Interaction of human adipose tissue-derived mesenchymal stromal cells with breast
cancer cells. Neoplasma 2011, 58:361-370.
3. Kucerova L, Skolekova S: Tumor microenvironment and the role of mesenchymal
stromal cells. Neoplasma 2013, 60:1-10.
59. USE OF NANO-SIZED REALGAR PARTICLES AND SOLUBLE ARSENIC IN TREATMENT
OF CANCER: KNOWLEDGE FROM TRADITIONAL CHINESE MEDICINE
Pastorek M. 1 , Balaz P. 2 , Bujnakova Z. 2 , Jakubikova J. 1,3 , Cholujova D. 1 , Gronesova P. 1 ,
Duraj J. 1 , Hunakova L. 1 , Sedlak J. 1
1
Cancer Research Institute, Slovak Academy of Sciences
2
Institute of Geotechnics, Slovak Academy of Sciences
3
Dana Farber Cancer Institute, Department of Medical Oncology, Boston, USA
Despite significant progress that has been made in field of cancer treatment, it still
remains an incurable disease. The use of natural compounds from traditional chinese
medicine and their application along with western knowledge is among a new emerging
efforts in cancer patient treatment. Xionghuang is a traditional remedy containing realgar
(As 4
S 4
) prepared by special procedure. Because of its very low solubility we have used
high-energy milling to prepare realgar in form of nanoparticles. Therefore the effect of
realgar was compared with ATO (arsenic trioxide) using the panel of melanoma cell lines.
Flow cytometry analysis showed time- and concentration-dependent cytotoxicity
of realgar nanoparticles and ATO solution that was proportional to concentration of
arsenic. Both tested compounds influenced cell cycle distribution with significant
induction of G2/M block. Despite postive and negative regulation of glutathione level
observed within different ranges of arsenic concentration, the use of BSO (inhibitor of
GSH synthesis) increased toxicity of realgar and ATO within whole concentration scale of
arsenic. Combined treatment of realgar or ATO with sulforaphane, the chemopreventive
compound known for modulation of GSH level, achieved synergistic toxic effect.
Analytical Cytometry VII 75