ABSTRACTS â ORAL PRESENTATIONS - AMCA, spol. s r.o.

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55. ACTIVITY OF ALDEHYDE-DEHYDROGENASE IN B-CELL AND PLASMA CELL SUBSETS OF MULTIPLE MYELOMA PATIENTS Pavla Vsianska 1,2,3 , Lucie Rihova 1,2 , Fedor Kryukov 1,2 , Miroslav Penka 1 , Roman Hajek 1,2 1 Dept. of Clinical Haematology, University Hospital Brno, Brno, Czech Republic, ZarbochovaP@seznam.cz 2 Babak Myeloma Group by Dept. of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic 3 Dept. of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic Multiple myeloma (MM) is characterized by the presence of clonal plasma cells (PC) arising from malignant transformed B-cells. It is still not clear which stage of B-cell differentiation is responsible for the development of MM and for eventual relapse after treatment, so nowadays there is an effort to identify the source of myeloma-initiating cells. Aldehyde-dehydrogenase (ALDH) is an intracellular enzyme catalysing degradation of aldehydes to protect the cell against a toxic damage (Russo and Hilton, 1988). This enzyme is active in hematopoietic stem and progenitor cells and its increased activity was detected also in some types of cancer stem cells (Shenoy et al., 2012; Rappa et al., 2013). The aim of this study was monitoring of ALDH activity in B-cell and plasma cell subpopulations in MM patients to identify potential source population of myeloma progenitors. Bone marrow (BM) of 10 newly diagnosed MM patients were analysed by 8-color flow cytometry. Identification of immature, transitional, naïve, memory (with/without isotype switch) and switched CD27 - B-cells, as well as plasmablasts and PCs (CD19 + PCs, CD19 - PCs and CD138 -/dim+ PCs) was performed according to expression of surface markers CD38, CD45, CD20, CD138, CD19, CD27 and IgM. Aldefluor assay was used to identify activity of ALDH in individual subsets and negative control with ALDH inhibitor was used in each sample. Rate of ALDH activity was assessed based on percentage of ALDH positive cells (%pos) and ratio of median fluorescence intensity (MFI) of ALDH and MFI of negative control. Statistical significance of differences in continuous variables among groups of patients was analysed using nonparametric Kruskal-Wallis or Mann-Whitney U test. For the robust analysis of continuous parameters relationship the Spearman correlation coefficient was adopted. There was found an decreasing trend of ALDH activity in B-cell development from immature to mature naïve B-cells, then the activity is stable until the stage of PC, where it increases again. Higher activity of ALDH in immature B-cells in comparison with naïve B-cells was found according to MFI ratio [1,42 (1,15-1,79) vs. 1,03 (0,72-1,59), p
42,0) vs. 1,5 (0,0-28), p
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Page 5 and 6: 25. PROFILING OF CHILDHOOD ACUTE LE
Page 7 and 8: more dominantly than Th1 and CD4 ce
Page 9 and 10: 29. INFLUENCE OF THE LEVEL OF CD133
Page 11 and 12: 36. B-CELL IMMUNOPHENOTYPING OF LAR
Page 13 and 14: possible mechanism behind risk alle
Page 15 and 16: isotype controls and FMO for CD14 a
Page 17 and 18: NEP developmental stages were disti
Page 19 and 20: 50. THE PROGRESSION OF HIV INFECTIO
Page 21 and 22: 52. PRŮKAZ REGULAČNÍCH T LYMFOCY
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Page 37 and 38: approaches how to detect and isolat
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Page 53 and 54: models. As manifested by compromise
Page 55 and 56: P11. NEW BIOACTIVE AND FLUORESCENT
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Page 59 and 60: stimulated cells. The physiological
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Page 63 and 64: chemotherapeutic drug LA-12. These
Page 65 and 66: aberrant expression, localization a
Page 67 and 68: P21. ASSESSMENT OF MITOCHONDRIAL FU
Page 69 and 70: P23. PTEROSTILBENE: COMPARISON OF A
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Page 73 and 74: In summary, we described different
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this process. Combined treatment wi
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Homolog 1) gene; previously identif
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egulate expression of different gen
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was harmful neither alone nor in co
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P34. PHARMACOLOGICAL INHIBITION OF
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novel chemotherapeutics with specif
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P38. EVIDENCE OF ABNORMAL PERIPHERA
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acid to 5-lipoxygenase. Similarly t
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P41. WHY CAN WE SEE DIFFERENT RADIO
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P42. ELUCIDATION OF CROSSTALK BETWE
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P44. INSTRUMENT SETTINGS FOR EUROFL
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e used to cultivate endothelial cel
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concentrations 0.15, 0.2, 0.3 and 0
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P49. NEW ANALOGS OF RHODAMINE Jiři
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P51. FLUORESCENCE-LABELED FERROMAGN
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could be achieved via the activatio
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28. Pp. 1565-1570. 2008. Dearden C:
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cells (SFC) using IFN-γ Elispot in
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FACSCantoII flow cytometer (Becton
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P59. EARLY DIAGNOSTICS OF CARTILAGE
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cross-reacting group (CREG) and sha
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the frequency of autoimmune disease
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non-covalent bonds. For a solution
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P65. OVULATION STIMULATION PROTOCOL
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Acknowledgements This work was supp
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P68. SUBPOPULATIONS OF B LYMPHOCYTE
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triggers a process of normal blood
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3 Department of Internal Medicine -
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P73. THE V-ATPASE-INHIBITOR BAFILOM
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P75. 5-FLUOROURACIL-SELECTED TUMOUR
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We conclude that decrease in the po
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Acknowledgements This work was supp
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P79. NEW TYPE OF PHOTOSENSITIZER IS
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P83. DIFFERENTIATION OF NEURAL CRES
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given cell type are missing and the
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Our results show that both p38α +/
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P90. TOLL-LIKE RECEPTOR 2 TRACKS TH
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for chemokines in attracting “inf
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in immune organs (Bursa of Fabricii
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ABSTRACTS â ORAL PRESENTATIONS - AMCA, spol. s r.o.