ABSTRACTS – ORAL PRESENTATIONS - AMCA, spol. s r.o.

výsledků získaných ze separovaných lymfocytů a plné krve).Hladiny regulačních T
lymfocytů byly sledovány u různých imunopatologických stavů (infekce MTB, alergie,
autoimunitní choroby léčené biologickou terapií, atd.).
Reference
Bluestone JA, Zang Q., Curr. Opin. Immunol. 2005, 17:638-642, Liu W., Putnam, AL., Xuyu
Z., et al., JEM, 2006, 203(7), 1701-1711)
Liu W, Putnam AL, Xu-Yu Z, Szot GL, Lee MR, Zhu S, Gottlieb PA, Kapranov P, Gingeras
TR, Fazekas de St Groth B, Clayberger C, Soper DM, Ziegler SF, Bluestone JA. CD127
expression inversely correlates with FoxP3 and suppressive function of human CD4+ T
reg cells. J Exp Med. 2006 Jul 10; 203(7):1701-11
Mazzucchelli M. and Scott K. Durum, Interleukin-7 receptor expression: intelligent
design. Nature reviews Immunology Vol 7, No 6, June 2007)
53. THE RESULTS INTERPRETATION OF BASIC LYMPHOCYTE SUBPOPULATIONS BY THE
VIEW OF FLOW CYTOMETRIST AND CLINICAL IMMUNOLOGIST
Marcela Vlková, Zdenka Pikulová
Department of Clinical Immunology and Allergology, St Anne’s University Hospital,
Faculty of Medicine, Masaryk University, Brno, Czech Republic
marcela.vlkova@fnusa.cz
Results of immunophenotyping of peripheral blood lymphocyte subsets may
give important information for the diagnosis and treatment of haematological or
immunological disorders as well as infectious diseases. For example, the number of
CD4+T-cells are used to define the stage of the human immunodeficiency virus infection,
the number of cytotoxic T-cells and their activation status may be an important tool for
the determination of viral infections such as cytomegalovirus or Epstein–Barr virus. It
is necessary to keep some basic terms of sample manipulation to be able to correctly
interpret the results of immunophenotyping of peripheral blood lymphocyte subsets
from flow cytometer.
The measurement process should meet the requirements of internal quality control.
First condition is properly collected blood: proper storage of sample, transportation
conditions, timely delivery to the laboratory. The second condition is the right
preparation and processing of samples for measurement. Third condition involves
functional and properly configured flow cytometer. Fourth important requirement is a
correctly measured sample.
If all these conditions are met we can proceed to the actual interpretation of cytometric
data. For this we need the determination of a range of reference values of lymphocyte
subpopulations depending on the patient’s age. At the birth the immune system is
functionally immature and undergoes a sequential development which is followed
as changing leukocytes and lymphocytes levels and percentage of lymphocyte
subpopulations. This development is genetically determinated and depends on
subsequent stimulation by antigen. These changes necessitate age-matched reference
Analytical Cytometry VII 67