ABSTRACTS – ORAL PRESENTATIONS - AMCA, spol. s r.o.

Our data demonstrate that simultaneous determination of the proliferative activity of
cells with CD4+ count seems to be more effective marker for assessing the degree of
functional immunodeficiency monitoring HIV patients than CD4+ count itself.
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51. MYSTERIES IN THE LABORATORY OF CELLULAR IMMUNOLOGY OR
WHAT WE CAN ENCOUNTER WHEN ANALYZING DATA FROM FLOW CYTOMETER
Doris Vokurková 1 , Pavlína Králíčková 1 , Eva Malá 1 , Jakub Novosad 1 , Pavel Rozsíval 2 , Eva
Pařízková 2
vokurkovad@lfhk.cuni.cz
1
Institute of Clinical Immunology and Allergology, Medical Faculty and Faculty Hospital,
Hradec Kralove, Charles University in Prague, Czech Republic
2
Department of Pediatrics, University Hospital, Charles University, Hradec Kralove,
Czech Republic
One of the advanced techniques that in recent years achieved significant technological
development is the method of flow cytometry. It plays a vital role in the immunological
and haematological laboratory and the number of tests for examining patients continues
to grow. A number of methods are validated, there are commercial kits for functional
tests, regular calibration is carried out, protocols for the methods are set, but still
sometimes we get results that deviate from the norm for other reasons than because of
pathological value of the result.
For example, how to interpret normal percentage values of metabolic flare in granulocytes
with a substantially lower MFI? How to explain a relatively good clinical condition of
a patient who according to the results of ​burstest and cytoplasmatic myeloperoxidase
should suffer from serious bacterial infections? Why, from time to time, during the
measurement of basic lymphocyte populations there appears „unbalanced“ twoparameter
dot-plot histograms and the compensation matrix of the given sample must
be adjusted? How to evaluate patients with recurrent high negative control in bazotest?
Even after several years of work with a flow cytometer we encounter very baffling cases
that are difficult to interpret and therefore they deserve to be discussed in more detail.
Analytical Cytometry VII 65