ABSTRACTS â ORAL PRESENTATIONS - AMCA, spol. s r.o.

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P93. FLOW CYTOMETRY STUDY OF IMMUNE RESPONSE AFTER ADMINISTRATION OF β-D-GLUCAN AND LOW DOSE OF T-2 TOXIN IN CHICKENS Viera Revajová 1 , Martin Levkut 1 , Viera Spišáková 1 , Zuzana Ševčíková 1 , Katarína Bobíková 1 , Eva Husáková 1 , Mária Levkutová 1 , Dominika Pejová 1 , Ema Paulovičová 2 , Mikuláš Levkut 1 1 University of Veterinary Medicine and Pharmacy, Slovak Republic; revajova@uvm.sk 2 Institute of Chemistry Slovak Academy of Science, Slovak Republic There is an increasing awareness of the hazard posed to both human and animal health by the presence of toxins produced by fungi in food and feed. Mycotoxins, where T-2 toxin is included, have a diversity of chemical structures which accounts for different biological effects. They can be carcinogenic, mutagenic, teratogenic, immunotoxic, etc. One way of trying to inhibit the uptake of mycotoxins from contaminated feed is the use of mycotoxin binders. Facing the relative inefficacy of the clay binders towards mycotoxins others than aflatoxins, natural organic binders, such as yeast and lactic acid bacteria, have been shown to bind the different mycotoxins strongly to cell wall components (Kolossova et al., 2009). Another beneficial effects of lactic acid bacteria and yeasts are nutritional values and improving of immunity. A significant amount of ß-glucan entered the proximal intestine shortly after ingestion. Its transit through the proximal intestine decreased during time with a simultaneous increase in the ileum. Despite low systemic blood levels (less than 0.5%), significant systemic immunomodulating effects in terms of humoral and cellular immune responses were demonstrated (Větvička et al., 2007). Based on in vitro studies, ß-glucans act on several immune receptors including Dectin-1, complement receptor (CR3) and TLR-2/6 and trigger a group of immune cells including macrophages, neutrophils, monocytes, natural killer cells and dendritic cells. As a consequence, both innate and adaptive response can be modulated by ß-glucans and they can also enhance opsonic and nonopsonic phagocytosis (Chan et al., 2009) and various types of interleukins. T-2 toxin was first isolated from the mould Fusarium tricinctum and belongs to nonmacrocyclic type A trichothecenes. After exposure by oral, dermal or inhalation route it can cause severe effects in various animal organs and tissues. In poultry, the toxic effects can be classified as genotoxic, cytotoxic, immunomodulatory.T-2 toxin can damage digestive system and liver, nervous system, skin and impairment poultry performance (Sokolović et al., 2008). Daily tolerated dose for T-2 toxin was established to 0.06 μg.kg -1 / body weight and limited dose in diet is 0.1 mg.kg -1 . T-2 toxin is a mycotoxin with immunomodulatory activity. Its mode of action is time- and dose-dependent. Immunosupression is the result of high doses. Immunostimulation is caused by low doses, and is evidenced by increase serum IgA and IgE antibodies because of rapid and transient activation of the genes responsible for the function of the immune system as well as genes important for inflammation response (Minervini et al., 2005). In the present study ß-D-glucan isolated from Candida albicans was perorally administered to chickens prior to feeding the diet artificially contaminated with low dose of T-2 toxin. The aim of the experiment was to determinate the changes of immunocompetent cells Analytical Cytometry VII 199
in immune organs (Bursa of Fabricii, spleen, tymus), intestine, and blood. To find out whether ß-D-glucan can induce immunologic response through T and B cell activation during T-2 toxin administration. For this purpose 4 group of chickens – control (C), ß-D-glucan (G), toxin (T) and combine ß-D-glucan+toxin (GT) – were investigated. Immunofenotyping of isolated lymphocytes by direct immunofluorescence method, double staining by mouse anti-chicken monoclonal antibody and flow cytometry for measuring of relative percentage were used. Determination of IgM+, IgG+ and IgA+ lymphocytes in Bursa of Fabricii showed increase (P
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ABSTRACTS - ORAL PRESENTATIONS 14.
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and (iii) siRNA-mediated knockdown
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25. PROFILING OF CHILDHOOD ACUTE LE
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more dominantly than Th1 and CD4 ce
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29. INFLUENCE OF THE LEVEL OF CD133
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36. B-CELL IMMUNOPHENOTYPING OF LAR
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possible mechanism behind risk alle
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isotype controls and FMO for CD14 a
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NEP developmental stages were disti
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50. THE PROGRESSION OF HIV INFECTIO
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52. PRŮKAZ REGULAČNÍCH T LYMFOCY
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values when analysing paediatric ly
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42,0) vs. 1,5 (0,0-28), p
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for application of proton therapy,
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effects will be also characterized
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Although multiple signalling pathwa
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an ability to recreate the tumour f
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incubated with non-filtered superna
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approaches how to detect and isolat
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progress in development of automate
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tissues is characteristic for a num
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kappaB. Beside that, we found OANO
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therapy in patients (Calderwood et
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difference in the effect caused eit
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4 CIC bioGUNE Technology Park of Bi
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P8. PROADIFEN (SKF-525A) ATTENUATES
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models. As manifested by compromise
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P11. NEW BIOACTIVE AND FLUORESCENT
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Acetylation of histones, along with
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stimulated cells. The physiological
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h induced very minor manifestations
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chemotherapeutic drug LA-12. These
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aberrant expression, localization a
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P21. ASSESSMENT OF MITOCHONDRIAL FU
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P23. PTEROSTILBENE: COMPARISON OF A
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Fam123b. Amer1 has been shown very
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In summary, we described different
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this process. Combined treatment wi
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Homolog 1) gene; previously identif
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egulate expression of different gen
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was harmful neither alone nor in co
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P34. PHARMACOLOGICAL INHIBITION OF
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novel chemotherapeutics with specif
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P38. EVIDENCE OF ABNORMAL PERIPHERA
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acid to 5-lipoxygenase. Similarly t
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P41. WHY CAN WE SEE DIFFERENT RADIO
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P42. ELUCIDATION OF CROSSTALK BETWE
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P44. INSTRUMENT SETTINGS FOR EUROFL
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e used to cultivate endothelial cel
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concentrations 0.15, 0.2, 0.3 and 0
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P49. NEW ANALOGS OF RHODAMINE Jiři
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Page 105 and 106: could be achieved via the activatio
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Page 109 and 110: cells (SFC) using IFN-γ Elispot in
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Page 115 and 116: cross-reacting group (CREG) and sha
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Page 119 and 120: non-covalent bonds. For a solution
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Page 131 and 132: P73. THE V-ATPASE-INHIBITOR BAFILOM
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Page 135 and 136: We conclude that decrease in the po
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Page 145 and 146: given cell type are missing and the
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ABSTRACTS â ORAL PRESENTATIONS - AMCA, spol. s r.o.