ABSTRACTS â ORAL PRESENTATIONS - AMCA, spol. s r.o.

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References Diwu Z., Lown J.W.: Phototherapeutic potential of alternative photosensitizers to porphyrins. – Pharmacol Ther. 63, 1-35, 1994. Robertson C.A., Hawkins Evans D., Abrahamse H.: Photodynamic therapy (PDT): A short review on cellular mechanisms and cancer research applications for PDT. – J Photochem Photobiol B. 96, 1-8, 2009. P80. HAEMATOPOIESIS IN VITRO UNDER HIF-1 MODULATION Markéta Hanáčková 1,2 , Kateřina Štefková 1 , Lukáš Kubala 1,2,3 , Jiří Pacherník 1,3 1 Institute of Experimental Biology, Faculty of Science, Masaryk University, Kotlarska 2, 611 37 Brno, Czech Republic, 17059@mail.muni.cz 2 Institute of Biophysics, Academy of Sciences of the Czech Republic v. v. i., Kralovopolska 135, 612 65 Brno, Czech Republic 3 International Clinical Research Center - Center of Biomolecular and Cellular Engineering, St. Anne‘s University Hospital Brno, Brno, Czech Republic Hypoxia through modulation of hypoxia inducible factors (HIF) controls induction and progression of haematopoiesis. Here we focus on the role of HIF family member HIF-1α in regulation of haematopoiesis from embryonic stem cells. We have observed that depletion of HIF-1α accelerates haematopoiesis in embryonic stem cells in vitro. In mutated cells both erythroid progenitors (BFU-E and CFU-E) appear earlier and expression of prohaematopoietic genes is increased compared to wild-type cells. Recently it has been observed that deferoxamine (DFO) and other Fe ions chelators not only induce differentiation but also shift myeloid differentiation to monocyte lineage in acute myeloid leukaemia (Callens et al., 2010). DFO through Fe ion chelation also stabilizes HIF-1. Therefore we tested the effect of classical HIF stabilizing drugs (CoCl 2 and DMOG) on adult and fetal haematopoiesis in vitro. In accordance with the effect of HIF-1α depletion, addition of CoCl 2 to the culture reduced erythropoiesis (decreased number of BFU-E and CFU-E) in both adult (bone marrow-derived) and fetal (fetal liver-derived) hematopoietic progenitors. Interestingly, CoCl 2 also decreased CFU-G together with CFU-GM but increased CFU-M number in fetal haematopoiesis in the same manner as DFO in acute myeloid leukemia study (Callens et al., 2010). In contrast, in adult hematopoietic progenitors CoCl 2 increased number of CFU-G and CFU-GM but decreased CFU-M. In summary, our results show HIF-1α dependent regulation of both overall haematopoiesis and myeloid lineage directed differentiation. Moreover, the results of myeloid differentiation in response to CoCl 2 in adult and fetal haematopoiesis may indirectly support recent hypothesis, that tumor cells may have the phenotype of fetal progenitors rather than the phenotype of adult lineage progenitors. Acknowledgments This work was supported by grants from MEYS CR MSM0021622430 and Cost CZ Analytical Cytometry VII 185
LD11015, from GAČR 13-29358S, and from the European Social Fund - projects OganoNET (CZ.1.07/2.4.00/31.0245) and HistoPARK (CZ.1.07/2.3.00/20.0185). LK was supported by the European Regional Development Fund - Project FNUSA-ICRC (No. CZ.1.05/1.1.00/02.0123). References Callens C, Coulon S, Naudin J, Radford-Weiss I, Boissel N, Raffoux E, Wang PH, Agarwal S, Tamouza H, Paubelle E, Asnafi V, Ribeil JA, Dessen P, Canioni D, Chandesris O, Rubio MT, Beaumont C, Benhamou M, Dombret H, Macintyre E, Monteiro RC, Moura IC, Hermine O.: Targeting iron homeostasis induces cellular differentiation and synergizes with differentiating agents in acute myeloid leukemia. - J Exp Med. 207(4):731-50, 2010 P81. SULFORAPHANE UPREGULATES CD44, CD105 AND CD90 EXPRESSION IN MSCS PRESENT IN BONE MARROW Hunakova L. 1 , Altanerova V. 2 , Sulikova G. 1 , Kaiserova K. 2 , Altaner C. 1 1 Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Slovak Republic 2 St. Elisabeth Oncological Institute, Bratislava, Slovak Republic It has been accepted that mesenchymal (stromal) stem cells (MSCs) present in bone marrow mononuclear cells concentrate are therapeutic cells involved in regenerative process. Emerging evidence suggest that most of the beneficial effects of MSCs could be explained by secretion of soluble factors contributing by paracrine fashion to the multiple effects including modulation of inflammatory and immune reactions, modulation of cell death and stimulation of endogenous progenitor cells. Sulforaphane (SFN) is one of naturally occurring cancer chemopreventive isothiocyanates found in cruciferous vegetables, consumption of which has been associated with reduced risk of cancer. We found that low concentrations of SFN that do not affect the viability of bone marrow-derived MSCs as shown by MTT assay, increase the surface expression of CD105, CD44 and CD90 proteins. Recently, CD44 and CD90 expression have been shown to be associated with the responsiveness of CLI patients to bone marrow mononuclear cell therapy. Thus SFN might enhance the quality of MSCs as reflected on the expression of cell surface markers and find utilization in regenerative medicine. Acknowledgement Financial support from the Slovak Grant Agency VEGA (grant 2/0177/11) and Slovak League against Cancer is gratefully acknowledged. 186 Analytical Cytometry VII
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ABSTRACTS - ORAL PRESENTATIONS 14.
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and (iii) siRNA-mediated knockdown
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25. PROFILING OF CHILDHOOD ACUTE LE
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more dominantly than Th1 and CD4 ce
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29. INFLUENCE OF THE LEVEL OF CD133
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36. B-CELL IMMUNOPHENOTYPING OF LAR
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possible mechanism behind risk alle
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isotype controls and FMO for CD14 a
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NEP developmental stages were disti
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50. THE PROGRESSION OF HIV INFECTIO
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52. PRŮKAZ REGULAČNÍCH T LYMFOCY
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values when analysing paediatric ly
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42,0) vs. 1,5 (0,0-28), p
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for application of proton therapy,
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effects will be also characterized
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Although multiple signalling pathwa
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an ability to recreate the tumour f
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incubated with non-filtered superna
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approaches how to detect and isolat
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progress in development of automate
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tissues is characteristic for a num
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kappaB. Beside that, we found OANO
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therapy in patients (Calderwood et
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difference in the effect caused eit
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4 CIC bioGUNE Technology Park of Bi
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P8. PROADIFEN (SKF-525A) ATTENUATES
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models. As manifested by compromise
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P11. NEW BIOACTIVE AND FLUORESCENT
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Acetylation of histones, along with
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stimulated cells. The physiological
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h induced very minor manifestations
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chemotherapeutic drug LA-12. These
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aberrant expression, localization a
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P21. ASSESSMENT OF MITOCHONDRIAL FU
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P23. PTEROSTILBENE: COMPARISON OF A
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Fam123b. Amer1 has been shown very
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In summary, we described different
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this process. Combined treatment wi
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Homolog 1) gene; previously identif
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egulate expression of different gen
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was harmful neither alone nor in co
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P34. PHARMACOLOGICAL INHIBITION OF
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novel chemotherapeutics with specif
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P38. EVIDENCE OF ABNORMAL PERIPHERA
Page 89 and 90: acid to 5-lipoxygenase. Similarly t
Page 91 and 92: P41. WHY CAN WE SEE DIFFERENT RADIO
Page 93 and 94: P42. ELUCIDATION OF CROSSTALK BETWE
Page 95 and 96: P44. INSTRUMENT SETTINGS FOR EUROFL
Page 97 and 98: e used to cultivate endothelial cel
Page 99 and 100: concentrations 0.15, 0.2, 0.3 and 0
Page 101 and 102: P49. NEW ANALOGS OF RHODAMINE Jiři
Page 103 and 104: P51. FLUORESCENCE-LABELED FERROMAGN
Page 105 and 106: could be achieved via the activatio
Page 107 and 108: 28. Pp. 1565-1570. 2008. Dearden C:
Page 109 and 110: cells (SFC) using IFN-γ Elispot in
Page 111 and 112: FACSCantoII flow cytometer (Becton
Page 113 and 114: P59. EARLY DIAGNOSTICS OF CARTILAGE
Page 115 and 116: cross-reacting group (CREG) and sha
Page 117 and 118: the frequency of autoimmune disease
Page 119 and 120: non-covalent bonds. For a solution
Page 121 and 122: P65. OVULATION STIMULATION PROTOCOL
Page 123 and 124: Acknowledgements This work was supp
Page 125 and 126: P68. SUBPOPULATIONS OF B LYMPHOCYTE
Page 127 and 128: triggers a process of normal blood
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Page 131 and 132: P73. THE V-ATPASE-INHIBITOR BAFILOM
Page 133 and 134: P75. 5-FLUOROURACIL-SELECTED TUMOUR
Page 135 and 136: We conclude that decrease in the po
Page 137 and 138: Acknowledgements This work was supp
Page 139: P79. NEW TYPE OF PHOTOSENSITIZER IS
Page 143 and 144: P83. DIFFERENTIATION OF NEURAL CRES
Page 145 and 146: given cell type are missing and the
Page 147 and 148: and HistoPARK (CZ.1.07/2.3.00/20.01
Page 149 and 150: Our results show that both p38α +/
Page 151 and 152: P90. TOLL-LIKE RECEPTOR 2 TRACKS TH
Page 153 and 154: for chemokines in attracting “inf
Page 155: in immune organs (Bursa of Fabricii
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