ABSTRACTS – ORAL PRESENTATIONS - AMCA, spol. s r.o.

P61. PARAMETERS OF CELLULAR IMMUNITY IN PATIENTS WITH RHEUMATIC DISEASES
DURING PREGNANCY
Gabriela Marková 1 , Jana Špajdelová 1 , Stanislava Blažíčková 1,2
1
Faculty of Health Care and Social Work, University of Trnava, Trnava, Slovak Republic;
gabimarkova@gmail.com
2
Laboratoria s.r.o., Piešťany, Slovak Republic
Rheumatoid arthritis and systemic lupus erythematosus have in part overlapping clinical
features, but behave strikingly differently during pregnancy. Pregnancy patients with SLE
have a double incidence of flare, whereas diminution of disease activity in observed
in patients with RA. Apparently, an immunomodulating mechanism is activated during
pregnancy that is responsible for opposite effect in activity of these autoimmune
diseases. Both antibody and cellular immunities are maintained. Since the immune
mechanisms plays a role in the pathogenesis of rheumatic diseases, we monitored
the changes in selected parameters of cellular immunity in 5 pregnant patients with
rheumatic diseases (2 SLE patients, 2 PsA patients and 1 JCA patient) in the 6 th and 9 th
months of pregnancy, a day after delivery and 2 months after delivery. The evaluated
parameters were: subpopulations of leucocytes in peripheral blood and their activity.
In the course of pregnancy, slight increase of T-lymphocytes, especially of helper CD4
lymphocytes was observed, as well as decrease of cytotoxic CD8 lymphocytes. After
childbirth the values of all these parameters went back to the standard. There were no
alterations observed in the numbers of NK cells either in the course of pregnancy or after
delivery. The expression of the CD69 and CD25 receptors on the lymphocytes stimulated
with phytohemaglutinin and CD2/CD2R monoclonal antibody decreased in pregnancy.
A possibility that, observed alterations in the cellular and non-specific immunities can
contribute in the immune homeostasis disorder in rheumatic patients after delivery,
cannot be excluded.
P62. DETERMINATION OF REGULATORY T CELLS IN PATIENTS WITH SELECTIVE IGA
DEFICIENCY
Jana Nechvatalova, Jiri Litzman, Marcela Vlkova
Department of Clinical Immunology and Allergology, St Anne’s University Hospital,
Faculty of Medicine, Masaryk University, Brno, Czech Republic; jana.nechvatalova@
fnusa.cz
Previous studies showed that several T- or B-lymphocyte abnormalities seen in the most
frequent symptomatic immunoglobulin deficiency, common variable immunodeficiency
(CVID), were also observed in a genetically related state - selective IgA deficiency (IgAD)
(Litzman et al., 2007, Nechvatalova et al., 2012). Patients with IgAD are asymptomatic
in most cases, or they may suffer from recurrent infections of the respiratory tract; also
Analytical Cytometry VII 161