ABSTRACTS – ORAL PRESENTATIONS - AMCA, spol. s r.o.

comparison to allergic children. The differences in intracellular TGF-beta were not
significant but the tendency to higher expression of TGF-beta in Tregs of healthy children
was obvious. Colonized children, even these suffering from allergy, showed better
functional markers of Tregs when compared with cells of non-colonized allergic children.
It is possible to conclude the insufficient function of Tregs in allergic children leads to
compensatory increase of their number. The immunomodulatory effect of probiotic E.
coli can be, at least partially, explained by its positive influence on Treg function.
Acknowledgements
This work was supported by Charles University research project PRVOUK P25/LF1/2,
GAUK259911, MSM0021620806.
References
Lodinová-Žádníková, R., Cukrowská, B., Tlaskalová-Hogenová, H.: Oral administration
of probiotic Escherichia coli after birth reduces frequency of allergies and repeated
infections later in life (after 10 and 20 years). Int Arch Allergy Immunol 2003; 131: 209–
211.
Lodinová-Zádníková, R., Prokesová, L., Kocourková, I., Hrdý, J., Zizka, J.: Prevention of
allergy in infants of allergic mothers by probiotic Escherichia coli. J. Int Arch Allergy
Immunol. 2010;153(2):201-6
P57. SIGNIFICANT ANTIGENS FOR DETECTION OF MINIMAL RESIDUAL DISEASE IN
PATIENTS WITH MANTLE CELL LYMPHOMA USING FLOW CYTOMETRY APPROACH
Jana Chovancová 1,2 , Tomáš Bernard 3 , David Šálek 3 , Andrea Janíková 3 , Jiří Mayer 1,2,3 ,
Michael Doubek 1,2,3
1
Faculty of Medicine Masaryk University, Kamenice 5, 625 000 Brno, Czech Republic;
jchovan@mail.muni.cz
2
Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno,
Czech Republic;
3
Dept. of Internal Medicine – Hematology and Oncology, University Hospital, Jihlavska
20, 625 00 Brno, Czech Republic
Background: Mantle cell lymphoma (MCL) is a B-cell neoplasm with an aggressive
clinical course typically characterized by CD5 + 19 + pathologic lymphocytes. The increase
of efficient treatment options has brought needs for early detection of complete
remission and minimal residual disease (MRD) (Medd et al., 2011). In this study, we
propose suitable combinations of CD markers that could lead to effective detection of
MRD in order to increase its sensitivity which means to improve the prediction of a
therapy response or continued remission.
Methods: Cohort of 28 consecutive patients with a new diagnosis of MCL together with
16 healthy donors were included into the analysis. Samples of peripheral blood were
tested using eight-color flow cytometry protocol. Cell surface antigens were stained with
fluorescence labeled monoclonal antibodies (anti-CD5, 10, 19, 20, 21, 22, 23, 24, 35,
38, 43, 45, 62L, 79b, 196 and 200). Flow cytometric acquisition was performed on a
Analytical Cytometry VII 155