ABSTRACTS – ORAL PRESENTATIONS - AMCA, spol. s r.o.

cells (SFC) using IFN-γ Elispot in comparison to controls. Moreover, a trend to higher
numbers of SFC was observed in these patients when compared to persons with a low
frequency of recurrences (n=7). Additionally, lack of difference was found in CD38 and
HLA-DR expression on circulating CD8+ T cells between the study groups.
Overall, these results indicate that systemic HSV-specific T cells are not suppressed
during recurrent genital herpes and the increased frequencies of these cells in the blood
are associated with the disease severity rather than with a protective role of these cells.
Acknowledgements
This work was supported by the grant of Charles University in Prague, PRVOUK/P24/LF1/3.
References
Wald A, Zeh J, Selke S, et al. Reactivation of genital herpes simplex virus type 2 infection
in asymptomatic seropositive persons. N Engl J Med 2000;342:844-50.
Zhu J, Koelle DM, Cao J, et al. Virus-specific CD8+ T cells accumulate near sensory nerve
endings in genital skin during subclinical HSV-2 reactivation. J Exp Med 2007;204:595-603.
P56. REDUCED ALLERGY INCIDENCE AND INCREASED ACTIVITY OF TREGS IN SIX YEAR
OLD CHILDREN OF ALLERGIC MOTHERS COLONIZED AT BIRTH BY PROBIOTIC E. COLI
Jiří Hrdý 1 , Ingrid Kocourková 2 , Rája Lodinová-Žádníková 2 , Ludmila Prokešová 1
1
Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University
in Prague, Prague, Czech Republic; jiri.hrdy@lf1.cuni.cz
2
Institute for the Care of Mother and Child, Prague, Czech Republic
Allergy belongs to one of the most common diseases with still increasing incidence.
In our previous work, we reported decreased allergy incidence in probiotic (Colinfant
New Born, Escherichia coli O83:K24:H31) colonized children [Lodinová-Žádníková, 2003,
Lodinová-Žádníková, 2010].
In this work, significantly decreased allergy incidence in colonized children of allergic
mothers (children with high risk for allergy development) in comparison to noncolonized
children of allergic mothers was observed. Allergy incidence in colonized
children of allergic mothers was comparable with allergy incidence in non-colonized
children of healthy mothers (children with relatively low risk for allergy development).
The beneficial effect of probiotics is well acknowledged but the mechanisms of their
actions are still unclear.
Because regulatory T cells (Tregs) are known to set the tolerance to relatively innocuous
environmental antigens (allergens), the proportion and functional properties of Tregs
from peripheral blood of colonized and non-colonized children in relation to their allergy
status were followed by flow cytometry. Surface markers of Tregs, intracellular FoxP3
and regulatory cytokines IL-10 and TGF-beta were tested.
The Treg percentage was lower in the blood of healthy children but the MFI (median of
fluorescence intensity) of FoxP3 reflecting better the functional potency was higher in
Tregs of healthy children in comparison with allergic ones. Furthermore, intracellular
presence of IL-10 was detected in higher percentage of Tregs of healthy children in
154 Analytical Cytometry VII