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P54. PHENOTYPE ANALYSIS OF DIFFERENTIATION: FROM HEMATOPOIETIC STEM
CELLS TOWARD B CELLS IN MONOCLONAL GAMMOPATHIES
Renata Bezděková 1 , Lucie Říhová 1,2 , Pavla Všianská 1,2,3 , Tamara Varmužová 1,2 , Zdenka
Pavková 1,2 , Renata Suská 2 , Miroslav Penka 2 , Roman Hájek 1,2,4
1
Babak Myeloma Group by Department of Pathological Physiology, Faculty of Medicine,
Masaryk University, Brno, Czech Republic; Renatta.Bezdekova@seznam.cz
2
Department of Clinical Haematology, University Hospital Brno, Brno, Czech Republic
3
Department of Experimental Biology, Faculty of Science, Masaryk University, Brno,
Czech Republic
4
Department of Clinical Hematology, University Hospital and Faculty of Medicine,
Ostrava University, Ostrava, Czech Republic
Background: Monoclonal gammopathies (MG) are characterized by presence of clonal
plasma cells (PC) producing monoclonal immunoglobulin (MIG). Non-malignant
monoclonal gammopathy of undetermined significance (MGUS) could progress
into malignant multiple myeloma (MM) (Pérez-Persona et al., 2007). The cause of
pathological transformation in MGs is still not known. Probably, there is a defect at the
level of memory B cells from which the PCs are subsequently developed (Matsui et al.,
2008). However, the early and immature form of B lymphocytes could be also involved.
The existence of myeloma-initiating cells is taken into consideration. These cells have
characteristic properties of stem cells and could be responsible for eventual relapse of
MM after treatment (Hosen, 2013).
Aim: Flow cytometry analysis and enumeration of different stem cell and B cell
subpopulations in monoclonal gammopathy subjects.
Subjects and methods: There were analysed 22 MM and 15 MGUS untreated subjects.
Whole bone marrow was incubated with followed antibodies (CD38, CD45, Lin, CD133,
CD34, CD117, CD243, CD138, HLA-DR, CD43, CD24, CD19, CD10, and CD20) and after
lysis analysed by flow cytometer. Different subpopulations were detected according to
their phenotype profile.
Results: There was found no difference in total number of CD34 + stem cells (including
early progenitors) when compared MGUS and MM. Number of CD117 - CD133 - lymphoid
progenitors from CD34 + stem cells was statistically significantly increased in MGUS than
in MM (14.5 vs. 8.5 %; p