Digoxigenin을 부착시킨 RNA in situ Hybridization의 ê°œì„ ëœ 연구 방법

Digoxigenin labeled in situ hybridization 105
SF
Tissue array: stomach carcinoma
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H I J
Fig. 2. RNA in situ hyrbidization, A-D; expression of elafin mRNA, A; tissue array slide composited with the cases of stomach carcinoma,
*; absent of specimen due to technical failure (× 3), most of cases are intensely positive, B; low magnification of a positive case of tissue
array. Tumor cells (arrows) and stromal fibrous (SF) tissue are distinguishable (× 25), C; human adult submandibular gland fixed in 4%
paraformaldehyde and prepared in RNase protection procedure, showing intense positive reaction in the acinar cells (arrows) and ductal
cells (D) (× 400), D; high magnification of panel b, elafin mRNA is intensely positive in the infiltrating tumor cells (arrows) (×400), E-F;
expression of snailfish clone (C90-171) (× 400), E; too intense positive reaction in the stromal fibrous tissue (arrows) when RNase A
treatment was omitted, F; nonspecific positive reaction was removed by RNase A treatment, G; expression of VEGF (vascular endothelial
growth factor) mRNA became very weak after RNase A treatment (× 400) H; double immunohistochemical staining of laminin
(arrows) and GFAP (glial fibrillary acid protein, arrowhead) I; in situ hybridization of laminin -chain followed by immunohistochemical
staining of GFAP (× 400), J; negative control of RNA in situ hybridization using universal sense RNA probe of elafin sense probe.