Soil priming effects following biochar incorporation - International ...
Soil priming effects following biochar incorporation - International ...
Soil priming effects following biochar incorporation - International ...
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The standard equation for determining δ 13 C (‰) is derived from:<br />
δ 13 C (‰) = [(R sample /R VPDB ) – 1] × 1000, Eqn. 1<br />
where R sample is the mass ratio of 13 C to 12 C and R VPDB is the international PDB<br />
limestone standard.<br />
The labelled 13 C (%) was then estimated from:<br />
CO 2 - 13 C (%) = (δ treatment - δC3) / (δC4 - δC3), Eqn. 2<br />
where CO 2 - 13 C (%) is the proportion of evolved CO 2 from C4 (Miscanthus <strong>biochar</strong>)<br />
materials, δ treatment is the δ 13 C (‰) in treatment, δC3 is the δ 13 C (‰) in control soil<br />
and δC4 is the δ 13 C (‰) from C4 (Miscanthus <strong>biochar</strong>) materials.<br />
Thus, the CO 2 -C produced by <strong>biochar</strong> during the incubation was calculated from:<br />
CO 2 - 13 C (μg g -1 soil) = CO 2 - 13 C (%) × total evolved CO 2 -C (μg g -1 soil) Eqn. 3
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