DNA Microarray Image Analysis - University of Illinois at Urbana ...
DNA Microarray Image Analysis - University of Illinois at Urbana ...
DNA Microarray Image Analysis - University of Illinois at Urbana ...
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Normaliz<strong>at</strong>ion<br />
• Normaliz<strong>at</strong>ion using control spots<br />
— insert spots <strong>of</strong> known intensities or genes <strong>of</strong> known expression level<br />
into a microarray slide<br />
• Normaliz<strong>at</strong>ion using regression analyses<br />
— within-slide normaliz<strong>at</strong>ion (loc<strong>at</strong>ion or scale),<br />
– (a) loc<strong>at</strong>ion global normaliz<strong>at</strong>ion (log(red/green) – normaliz<strong>at</strong>ion<br />
factor)<br />
– (b) loc<strong>at</strong>ion intensity dependent normaliz<strong>at</strong>ion (log(red/green) –<br />
normaliz<strong>at</strong>ion factor as a function <strong>of</strong> spot intensity),<br />
– (c) loc<strong>at</strong>ion within-print-tip-group normaliz<strong>at</strong>ion (log(red/green) –<br />
grid dependent normaliz<strong>at</strong>ion factor as a function <strong>of</strong> spot<br />
intensity) and<br />
– (d) scale normaliz<strong>at</strong>ion (modeling spread <strong>of</strong> various print-tip<br />
groups)<br />
— paired-slide normaliz<strong>at</strong>ion (dye-swap),<br />
— multiple slide normaliz<strong>at</strong>ion<br />
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