DNA Microarray Image Analysis - University of Illinois at Urbana ...
DNA Microarray Image Analysis - University of Illinois at Urbana ...
DNA Microarray Image Analysis - University of Illinois at Urbana ...
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D<strong>at</strong>a Quantific<strong>at</strong>ion<br />
• D<strong>at</strong>a quantific<strong>at</strong>ion (or spot fe<strong>at</strong>ure extraction) refers to<br />
extracting descriptive values <strong>of</strong> foreground and background<br />
pixels for each spot.<br />
• Ideally, extracted descriptors (also called fe<strong>at</strong>ures or<br />
<strong>at</strong>tributes) should be directly proportional to the mRNA<br />
quantity in the solution th<strong>at</strong> was deposited in a spot, and<br />
should represent the deposited gene expression level.<br />
• In reality, fluorescent intensity measurements in each<br />
channel might be scaled or distorted differently according<br />
to some linear or non-linear functions during d<strong>at</strong>a<br />
prepar<strong>at</strong>ion steps.<br />
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