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DNA Microarray Image Analysis - University of Illinois at Urbana ...

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I2K Selecting Valid Pixels – Topology Method<br />

• Method:<br />

— 1. Perform connectivity analysis <strong>of</strong> signal pixels<br />

— 2. Estim<strong>at</strong>e spot radius from the connected set <strong>of</strong> pixels.<br />

— 3. If the estim<strong>at</strong>ed radius devi<strong>at</strong>es from the expected<br />

radius by more than a user specified percentage <strong>of</strong> the<br />

expected spot radius then the spot is elimin<strong>at</strong>ed.<br />

• Topology screening criterion is aimed <strong>at</strong> elimin<strong>at</strong>ing spots<br />

with scr<strong>at</strong>ches.<br />

Original <strong>Image</strong><br />

Mask <strong>Image</strong> – Topology Screening<br />

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