DNA Microarray Image Analysis - University of Illinois at Urbana ...
DNA Microarray Image Analysis - University of Illinois at Urbana ...
DNA Microarray Image Analysis - University of Illinois at Urbana ...
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I2K Selecting Valid Pixels – Loc<strong>at</strong>ion & Size Method<br />
• Method:<br />
— 1. Estim<strong>at</strong>e background (sample mean and standard devi<strong>at</strong>ion ) and<br />
compute threshold<br />
— 2. Sp<strong>at</strong>ial centroid <strong>of</strong> pixels th<strong>at</strong> are above threshold define a new spot<br />
center.<br />
— 3. Background estim<strong>at</strong>es are iter<strong>at</strong>ively improved<br />
— 4. We identify spots th<strong>at</strong> have a spot centroid outside <strong>of</strong> the range [grid<br />
cell center ± 0.25*grid cell size]<br />
• Loc & Size method elimin<strong>at</strong>es tapering spots (comets) and partially missing<br />
spots.<br />
Original <strong>Image</strong> Mask <strong>Image</strong> – Loc<strong>at</strong>ion & Size Screening<br />
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