DNA Microarray Image Analysis - University of Illinois at Urbana ...
DNA Microarray Image Analysis - University of Illinois at Urbana ...
DNA Microarray Image Analysis - University of Illinois at Urbana ...
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Criteria for Assessing Background and Foreground Intensities<br />
• Background intensity vari<strong>at</strong>ions<br />
• Foreground and background intensity uniformity<br />
q<br />
STAT<br />
FRG<br />
• Foreground intensity s<strong>at</strong>ur<strong>at</strong>ion<br />
• Signal-to-noise r<strong>at</strong>io<br />
q<br />
µ<br />
GLOBAL<br />
GLOBAL<br />
LOC& GLOB 1 BKG<br />
LOC& GLOB 2<br />
BKG<br />
BKG<br />
= ; q<br />
LOCAL GLOBAL BKG<br />
=<br />
LOCAL GLOBAL<br />
µ<br />
BKG<br />
+ µ<br />
BKG<br />
mBKG + mBKG<br />
σ<br />
FRG<br />
ABS<br />
= 1− ,max ,min ,max ,min<br />
1 I FRG<br />
− I FRG ABS<br />
BKG BKG<br />
FRG<br />
;<br />
BKG<br />
1<br />
I −<br />
q = − q = −<br />
I<br />
µ<br />
Range<br />
q<br />
FRG<br />
CONT<br />
SATURATION<br />
count<br />
= 1−<br />
s<strong>at</strong>ur<strong>at</strong>ed<br />
count<br />
m<br />
( ) ( )<br />
Range<br />
all<br />
q<br />
CATEG<br />
SATURATION<br />
=<br />
1; if count < T %<br />
s<strong>at</strong>ur<strong>at</strong>ed<br />
0; if count ≥T<br />
%<br />
s<strong>at</strong>ur<strong>at</strong>ed<br />
MEAN<br />
MEDIAN<br />
q = µ /( µ + µ ); q = m /( m + m )<br />
SNR FRG FRG BKG SNR FRG FRG BKG<br />
68
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