DNA Microarray Image Analysis - University of Illinois at Urbana ...
DNA Microarray Image Analysis - University of Illinois at Urbana ...
DNA Microarray Image Analysis - University of Illinois at Urbana ...
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<strong>Microarray</strong> Grid Alignment: Previous Work<br />
• Enhancements <strong>of</strong> templ<strong>at</strong>e-based m<strong>at</strong>ching:<br />
— autom<strong>at</strong>ic refinement search for a grid loc<strong>at</strong>ion given size and<br />
spacing <strong>of</strong> spots<br />
• Enhancements <strong>of</strong> d<strong>at</strong>a-driven m<strong>at</strong>ching:<br />
— Adaptive threshold segment<strong>at</strong>ion<br />
• The problem <strong>of</strong> irregular grid:<br />
— templ<strong>at</strong>e-based approaches fail without manual adjustment<br />
— d<strong>at</strong>a-driven approaches are capable <strong>of</strong> finding irregular grids<br />
but are prone to misalignment due to spurious or missing spots<br />
and are also dependent on many parameters.<br />
• Some s<strong>of</strong>tware packages: GenePix, QuantArray, Array Vision,<br />
ScanAnalyze, Spot <strong>Image</strong> <strong>Analysis</strong> and Dapple.<br />
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