DNA Microarray Image Analysis - University of Illinois at Urbana ...
DNA Microarray Image Analysis - University of Illinois at Urbana ...
DNA Microarray Image Analysis - University of Illinois at Urbana ...
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31<br />
<strong>Microarray</strong> Grid Alignment: Previous Work<br />
Approaches:<br />
• The Affymetrix chips approach –<br />
— Pros: the alignment problem was simplified and hence the grid<br />
alignment became more accur<strong>at</strong>e.<br />
— Cons: the Affymetrix technology has been much more expensive<br />
than the technology with co<strong>at</strong>ed glass slides.<br />
• Templ<strong>at</strong>e-based approach: the most prevalent in existing s<strong>of</strong>tware<br />
packages, e.g., GenePix Pro by Axon Instruments, ScanAlyze or<br />
GridOnArray by Scanalytics.<br />
— Pros: Incorpor<strong>at</strong>es knowledge about ideal grid<br />
— Cons: manual alignment<br />
• D<strong>at</strong>a-driven approach:<br />
— image segment<strong>at</strong>ion<br />
— st<strong>at</strong>istical analysis <strong>of</strong> 1D image projections.<br />
— Pros: autom<strong>at</strong>ic alignment<br />
— Cons: accuracy and robustness, e.g., missing spots, noise
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