DNA Microarray Image Analysis - University of Illinois at Urbana ...
DNA Microarray Image Analysis - University of Illinois at Urbana ... DNA Microarray Image Analysis - University of Illinois at Urbana ...
Variations of Background • Slide washing • Examples of background noise that could be modeled with PDF models of noise. (Normal PDF – left and Student’s t PDF –right). 20
Variation of Spot Morphology • Spot morphologies other than circular • Spatial and morphological variations of spots (from left to right, top row first): (a) a regular spot, (b) an inverse spot or a ghost shape, (c) a spatially deviating spot inside of a grid cell, (d) a spot radius deviation, (e) a tapering spot or a comet shape, (f) a spot with a hole or a doughnut shape, (g) a partially missing spot and (h) a scratched spot. 21
- Page 1 and 2: February 4, 2005 DNA Microarray Ima
- Page 3 and 4: Publications • Journals: — Bajc
- Page 5 and 6: Microarray Problem: Major Objective
- Page 7 and 8: Input and Output of Microarray Data
- Page 9 and 10: Types of Expected Microarray Data M
- Page 11 and 12: 11 Microarray Data Processing Workf
- Page 13 and 14: DNA Microarray Image Analysis • T
- Page 15 and 16: Ideal Microarray Image? 1. Ideal cD
- Page 17 and 18: Microarray Image Technologies • A
- Page 19: Variations of Grid Geometry • Rot
- Page 23 and 24: Examples: Spatially Varying Backgro
- Page 25 and 26: 25 Examples: Spatial Resolution, Li
- Page 27 and 28: IMAGE ANALYSIS: MICROARRAY GRID ALI
- Page 29 and 30: Grid Alignment: Application Domains
- Page 31 and 32: 31 Microarray Grid Alignment: Previ
- Page 33 and 34: Microarray Grid Alignment: Previous
- Page 35 and 36: 35 Grid Alignment Algorithm Overvie
- Page 37 and 38: Image Down-Sampling • Design of r
- Page 39 and 40: Vertical and Horizontal Line Score
- Page 41 and 42: Optional Regularity Enforcement •
- Page 43 and 44: Processing Multiple Grids Line Disc
- Page 45 and 46: Spot Size & Spot Density 45 •Radi
- Page 47 and 48: Missing Spots The fewer the spots i
- Page 49 and 50: Down-sampling •Experimental resul
- Page 51 and 52: Grid Alignment Properties Color Inv
- Page 53 and 54: Multiple Grids: Semi-Automated vs.
- Page 55 and 56: 55 MICROARRAY FOREGROUND SEPARATION
- Page 57 and 58: Foreground Separation Using Spatial
- Page 59 and 60: Foreground Separation Using Intensi
- Page 61 and 62: Foreground Separation From Multi-Ch
- Page 63 and 64: Step 1: Separating Spots from Backg
- Page 65 and 66: Goals and Objectives of Quality Ass
- Page 67 and 68: Quality Assessment: Spot Examples
- Page 69 and 70: Criteria for Assessing Morphologica
Vari<strong>at</strong>ions <strong>of</strong> Background<br />
• Slide washing<br />
• Examples <strong>of</strong><br />
background noise<br />
th<strong>at</strong> could be<br />
modeled with PDF<br />
models <strong>of</strong> noise.<br />
(Normal PDF – left<br />
and Student’s t PDF<br />
–right).<br />
20
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