DNA Microarray Image Analysis - University of Illinois at Urbana ...
DNA Microarray Image Analysis - University of Illinois at Urbana ...
DNA Microarray Image Analysis - University of Illinois at Urbana ...
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<strong>Microarray</strong> <strong>Image</strong> Technologies<br />
• Affymetrix chips<br />
• Uses photolithography and solid-phase chemistry<br />
to produce arrays containing hundreds <strong>of</strong><br />
thousands <strong>of</strong> oligonucleotide probes packed <strong>at</strong><br />
extremely high densities.<br />
• Single-, double- or multi-fluorescent c<strong>DNA</strong><br />
microarray images<br />
• Variable substr<strong>at</strong>es: co<strong>at</strong>ed glass slides or nylon<br />
membrane or 2D gel m<strong>at</strong>erials.<br />
• Example: 532nm (red) and 632nm (green)<br />
wavelengths forming two channels.<br />
• <strong>Image</strong>s obtained by other labeling schemes, for<br />
example, with or without radio-isotopic labels lead<br />
to images with bright background and dark spots.<br />
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