DNA Microarray Image Analysis - University of Illinois at Urbana ...
DNA Microarray Image Analysis - University of Illinois at Urbana ...
DNA Microarray Image Analysis - University of Illinois at Urbana ...
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Sources <strong>of</strong> <strong>Microarray</strong> <strong>Image</strong> Vari<strong>at</strong>ions<br />
• The c<strong>DNA</strong> technology is a complex electrical-optical-chemical<br />
process th<strong>at</strong> spans c<strong>DNA</strong> slide fabric<strong>at</strong>ion, mRNA prepar<strong>at</strong>ion,<br />
fluorescence dye labeling, gene hybridiz<strong>at</strong>ion, robotic<br />
spotting, green and red fluorophores excit<strong>at</strong>ion by lasers,<br />
imaging using optics, slide scanning, analog to digital<br />
conversion using either charge-coupled devices (CCD) or<br />
photomultiplier tubes (PMT), and finally image storage and<br />
archiving.<br />
• Vari<strong>at</strong>ions: technologies, microarray image channels, file<br />
form<strong>at</strong>s, d<strong>at</strong>a accuracy, grid geometry, background, spot<br />
morphology, foreground and background intensity<br />
• Comput<strong>at</strong>ional Requirements: repe<strong>at</strong>ability (parameter “free”<br />
algorithms), sufficient storage and comput<strong>at</strong>ional resources<br />
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