Cholesterol (Liquid) Reagent Set - BrandSD

Cholesterol (Liquid)
Reagent Set
Example: Abs. (Patient) = 0.40, Abs. (Standard) = 0.32, Concentration of
Standard = 200 mg/dl
0.40 x 200 = 250 mg/dl
0.32
Quality Control
Serum controls with known normal and elevated values should be run
routinely to monitor the validity of the reaction. These controls should be run
at least with every working shift in which Cholesterol assays are performed.
It is recommended that each laboratory establish their own frequency of
control determination.
Expected Values 11
Recommended Range:
Desirable Cholesterol:
Borderline-High Cholesterol:
High Cholesterol:
240mg/dl
Manufactured by Pointe Scientific, Inc.
5449 Research Drive, Canton, MI 48188
European Authorized Representative:
Obelis s.a.
Boulevard Général Wahis 53
1030 Brussels, BELGIUM
Tel: (32)2.732.59.54 Fax: (32)2.732.60.03 email: mail@obelis.net
Performance
1. Linearity: 700 mg/dl
2. Comparison: A comparison between this procedure and a similar dry
powder reagent produced a regression equation of y = 1.02x + 0.8 with
a correlation coefficient of 0.999.
3. Precision:
Within Run
Run to Run
Mean S.D. C.V.% Mean S.D. C.V.%
157 1.1 0.7 155 1.3 0.8
224 1.7 0.8 227 2.4 1.1
4. Specificity: Cholesterol oxidase is not totally specific for cholesterol.
Other analogs of cholesterol. Other analogs of cholesterol
(dihydrocholesterol, 7-dehydrocholesterol, 20-hydroxycholesterol, etc.)
are also oxidized. These analogs do not normally occur in any
appreciable amounts in serum.
References
1. Lieberman, C., Ber. 18:1803 (1885).
2. Burchard, H., Chem. Fentr. 61:25 (1890).
3. Flegg, H.M., Ann. Clin. Biochem. 10:79 (1973).
4. Richmond, W., Scand. J. Clin. Lab. Invest. 29:Suppl. 26, abstr. 3:25
(1972).
5. Allain, C.C., et al, Clin. Chem. 20:470 (1974).
6. Roeschlau, P., et al, Z. Klin. Chem. Klin. Biochem 12:226 (1974).
7. Trinder, P., Ann. Clin. Biochem. 6:24 (1969).
8. Perlstein, M.T., et al, J. Microchem. 22:403 (1977).
9. Witte, D.L., et al, Clin. Chem. 20:1282 (1974).
10. Young, D.S. et al, Clin. Chem. 21:1D (1975).
11. National Institute of Health Publication No. 88-2926 “Detection,
Evaluation, and Treatment of High Cholesterol in Adults”, November
(1987).
Rev. 4/09 P803-C7510-01