Biosafety Manual PDF - Lawrence Berkeley National Laboratory
Biosafety Manual PDF - Lawrence Berkeley National Laboratory
Biosafety Manual PDF - Lawrence Berkeley National Laboratory
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<strong>Biosafety</strong> <strong>Manual</strong><br />
IBC-approved version (May 18, 2010)<br />
• Area-specific PPE requirements must be established for all Technical Areas (e.g.,<br />
laboratories) and must be posted on the LBNL entrance placard. Minimum PPE for<br />
laboratories where biological materials are stored or handled includes safety protective<br />
eyewear, long pants, and closed-toe shoes. Area PPE requirements apply to the entire<br />
Technical Area unless an exception is granted in accordance with the procedure<br />
described in PUB-3000, Chapter 19, Appendix A.<br />
• Activity- or operation-specific PPE requirements are assessed and defined in the<br />
<strong>Biosafety</strong> Work Authorization, which covers what PPE must be used (e.g., gloves,<br />
laboratory coats, and safety glasses) and any maintenance (e.g., laundering) or disposal<br />
requirements.<br />
General requirements and conditions for use of PPE related to biosafety include:<br />
• The supervisor or work lead is responsible for:<br />
o Determining what PPE is required to prevent occupational exposure<br />
o Providing at no cost to an employee the PPE required by this section or specified in<br />
the <strong>Biosafety</strong> Work Authorization. This PPE must be readily available in appropriate<br />
sizes.<br />
o Ensuring that employees and visitors properly use and store required PPE<br />
• The EH&S Division is available to assist supervisors or work leads in evaluating work<br />
activities and selecting appropriate PPE.<br />
• Employees and visitors are responsible for using PPE when required and whenever the<br />
work poses a reasonable probability of eye injury or exposure.<br />
• In general, removed PPE must be:<br />
o Decontaminated when needed, or<br />
o Disposed of in accordance with LBNL medical/biohazardous, hazardous, and<br />
radiological waste management requirements.<br />
• PPE that protects against exposure to BBP materials is considered appropriate if it does<br />
not permit BBP material (e.g., human blood or cell culture solution) to pass through the<br />
employee's work clothes, street clothes or undergarments, skin, eyes, or other mucous<br />
membranes under normal conditions of use and for the duration of time in which the<br />
PPE will be worn.<br />
5.4.1 Body Protection<br />
Protective laboratory clothing is a garment such as a lab coat, gown, smock,<br />
or uniform designed to keep personal clothing, forearms, or other exposed<br />
bodily surfaces protected from contamination by biological materials or<br />
exposure to other hazards. The term "protective laboratory clothing” typically<br />
applies to garments worn in the laboratory, but may also apply to garments<br />
worn in nonlaboratory work (e.g., health care).<br />
The following biosafety criteria are applicable to wearing protective laboratory clothing:<br />
• Protective laboratory clothing should be worn to prevent contamination of personal<br />
clothing when working at BL1.<br />
• Protective laboratory clothing must be worn when working at BL2 or when working with<br />
RG2 or other hazardous materials. This clothing must be removed and left in the<br />
laboratory before leaving for nonlaboratory areas (e.g., cafeteria, library, administrative<br />
offices).<br />
Printed copies are not official versions of this manual. Before using the printed copy, verify that it is the most current version.<br />
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