Biosafety Manual PDF - Lawrence Berkeley National Laboratory
Biosafety Manual PDF - Lawrence Berkeley National Laboratory
Biosafety Manual PDF - Lawrence Berkeley National Laboratory
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<strong>Biosafety</strong> <strong>Manual</strong><br />
IBC-approved version (May 18, 2010)<br />
Present<br />
at<br />
LBNL<br />
<strong>Biosafety</strong><br />
Level (BL)<br />
Table 8<br />
<strong>Laboratory</strong> <strong>Biosafety</strong> Containment Levels<br />
<strong>Biosafety</strong> Level Definition<br />
yes 1 BL1 is suitable for work involving agents of unknown or minimal<br />
potential hazard to laboratory personnel and the environment, 1 or<br />
work with defined and characterized strains of viable microorganisms<br />
not known to consistently cause disease in healthy adult humans. 2<br />
2 BL2 is suitable for laboratory work involving agents of moderate<br />
potential hazard to personnel and the environment. 1 Primary hazards<br />
to personnel working with these agents relate to accidental<br />
percutaneous or mucous membrane exposures, or ingestion of<br />
infectious materials. 2<br />
no 3 BL3 is applicable to facilities in which work is conducted with<br />
indigenous or exotic agents that may cause serious or potentially<br />
lethal disease as a result of exposure by the inhalation route. 3<br />
4 BL4 is required for work with dangerous and exotic agents that pose<br />
a high individual risk of life-threatening disease, aerosol transmission,<br />
or related agents having an unknown transmission risk. 3<br />
Footnotes:<br />
1 NIH Guidelines<br />
2 BMBL, fifth edition, Section III<br />
3 BMBL, fifth edition, Section IV<br />
When developing the <strong>Biosafety</strong> Work Authorization, the appropriate laboratory BL must be<br />
selected after conducting the risk assessment. Typical BLs used for various materials and<br />
agents are listed in Sections 4.4.1.1 and 4.4.1.2 of this manual. The final BL(s) are determined<br />
by the IBC.<br />
4.4.1.1 <strong>Laboratory</strong> <strong>Biosafety</strong> Level 1<br />
BL1 laboratories are not necessarily separated from the general traffic patterns in the building.<br />
Work is typically conducted on open benchtops using standard microbiological practices.<br />
Special containment equipment or facility design is not required, but may be used as<br />
determined by a risk assessment. <strong>Laboratory</strong> personnel must have specific training in the<br />
procedures conducted in the laboratory and must be supervised by a scientist trained in<br />
microbiology or a related science.<br />
Printed copies are not official versions of this manual. Before using the printed copy, verify that it is the most current version.<br />
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