Biosafety Manual PDF - Lawrence Berkeley National Laboratory

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Biosafety Manual IBC-approved version (May 18, 2010) necessary to prevent contamination of the laboratory with the agents being handled and contamination of the work with agents from the environment. See Appendix D for common GMP. Supervisors and work leads are responsible for selecting and instructing workers on the specific GMP needed to conduct the work, or additional safety practices needed for specific agents or procedures. 4.2 Safety and Personal Protective Equipment Worker exposure to infectious agents may be prevented by the use of standard and activityspecific safety and personal protective equipment (PPE) as primary barriers or controls. The need for additional activity-specific safety equipment or PPE must be determined during risk assessment, and any equipment needed for safety should be included in the Biosafety Work Authorization. Standard safety equipment and PPE are equipment controls listed as BL containment criteria in BMBL and the NIH Guidelines. They provide primary barriers that prevent worker exposure to infectious agents. See Section 4.4 of this manual for additional information on BL containment categories and criteria. These standard equipment requirements, along with requirements from other biosafety standards, are used and customized for the work to be conducted. They are also summarized in the LBNL Biosafety Work Authorization. Standard safety equipment and PPE are applicable to most work with biological materials at LBNL. The following types of standard equipment and PPE are further discussed in Appendix C and Section 5.0 of this manual: • biosafety cabinets • PPE • other physical containment devices such as centrifuge safety cups 4.3 Facility Design and Construction Facility design and equipment provide secondary barriers that protect laboratory workers, persons outside the laboratory, the public, and the environment from potentially hazardous materials or agents that may be accidentally released from the laboratory. Standard facilites provide secondary barriers. Source: HHS CDC Office of Health and Safety. LBNL designs and operates its facilities where work with biological materials is conducted in accordance with applicable standard facilities criteria. Standard facilities are design features, materials, and equipment incorporated into the laboratory or facility in accordance with BL containment criteria stated in BMBL and the NIH Guidelines (see Section 4.4 for more information). Printed copies are not official versions of this manual. Before using the printed copy, verify that it is the most current version. 32
Biosafety Manual IBC-approved version (May 18, 2010) Standard BL1 and BL2 laboratory facility barriers are sufficient to control most work at LBNL. This is because risks related to most work are associated with direct contact with materials or agents in standard laboratories. Examples of standard facility barriers and equipment are listed below. See Appendix C for standard laboratory facilities criteria that summarize how these barriers must be employed. See Section 5.0 for additional information on each of these topics. • doors • sinks • cleanable surfaces and furnishings • window screens • ventilation and biosafety cabinets • vacuum line filters and traps • eyewashes • autoclaves If the risk assessment indicates that there is a risk of exposure to an infectious aerosol, then higher levels of safety equipment and PPE (primary barriers) or multiple secondary facilities barriers are necessary. Multiple secondary facilities barriers are not typically needed at LBNL. Some standard facility barriers are summarized in the Biosafety Work Authorization. Any additional special facility barriers that are required should also be included in the authorization. 4.4 Biosafety Containment Levels and Criteria LBNL requires researchers who work with biological materials to implement containment controls in accordance with an established biosafety level (BL). BL is a standard combination of practices and techniques, safety equipment, and facilities to safely contain biohazardous materials or agents to be used in the work, as specified by BMBL or the NIH Guidelines. Work at LBNL requires routine application of BLs developed for biological laboratories, and occasional application of BLs developed for other types of work such as large-scale recombinant operations. BLs for laboratories are presented in the next section. BLs for largescale, plant, and animal uses are presented in Section 4.4.2 this manual. The appropriate BL must be selected once the risk assessment has been completed. The final BL determination should consider all aspects of the work, hazards, and controls. The principal investigator (PI) or supervisor should propose the appropriate BL(s) when submitting the authorization for review. The final BL(s) are determined by the Institutional Biosafety Committee (IBC). 4.4.1 Laboratory Containment Levels Containment controls for laboratory biosafety are categorized into four BLs. Definitions of each laboratory biosafety level (BL) are provided in Table 8. Work at LBNL is commonly conducted at BL1 or BL2, while work at BL3 or BL4 is not currently conducted. Laboratory work at LBNL must be conducted in accordance with the standard and special work practices, safety equipment, and facility requirements noted in the laboratory BL1 and BL2 criteria listed in Appendix C. Printed copies are not official versions of this manual. Before using the printed copy, verify that it is the most current version. 33
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