Biosafety Manual PDF - Lawrence Berkeley National Laboratory

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Biosafety Manual IBC-approved version (May 18, 2010) Biosafety Manual Contents 1.0 Introduction 1.1 Policy 1.2 Purpose 1.3 Scope 1.4 Manual Administration and Presentation 1.5 Terms, Acronyms, and Abbreviations 1.6 Roles, Responsibilities, and Whom to Call 2.0 Starting and Conducting Work Safely 3.0 Work and Risk Assessment 3.1 LBNL Assessment and Authorization Processes 3.2 Biosafety Risk Assessment Process 3.3 Material or Agent Hazards and Requirements 3.1.1 Risk Group Classification 3.3.2 Pathogenic Agents and Toxins 3.3.2.1 Pathogen and Toxin Information and Guidance 3.3.2.2 Human Pathogens 3.3.2.3 Plant and Animal Pathogens 3.3.2.4 Biological Toxins 3.3.2.5 Select Agents and Toxins 3.3.2.6 Prions 3.3.3 USDA-Regulated Materials, Organisms, and Agents 3.3.4 Bloodborne Pathogens and Human Materials 3.3.5 Recombinant Materials, Organisms, and Agents 3.3.6 Animals 3.4 Laboratory Procedure Hazards 3.5 Worker Competence and Health 4.0 Biosafety Principles and Levels 4.1 Laboratory Practices 4.2 Safety and Personal Protective Equipment 4.3 Facility Design and Construction 4.4 Biosafety Containment Levels and Criteria 4.4.1 Laboratory Containment Levels 4.4.1.1 Laboratory Biosafety Level 1 4.4.1.2 Laboratory Biosafety Level 2 4.4.2 Additional Containment Categories 4.4.2.1 Recombinant Large-Scale Containment Levels 4.4.2.2 Recombinant Plant Containment Levels 4.4.2.3 Vertebrate Animal Containment Levels 4.4.2.4 Arthropod Containment Levels Printed copies are not official versions of this manual. Before using the printed copy, verify that it is the most current version. 2
Biosafety Manual IBC-approved version (May 18, 2010) 5.0 Specific Biosafety Controls 5.1 Work Authorizations 5.2 Training, Instruction, and Qualification 5.2.1 Job Hazards Analysis 5.2.2 Training Courses and Tracking 5.2.3 Job-Specific Instruction, Information, and Practices 5.3 Occupational Health and Immunization 5.4 Personal Protective Clothing and Equipment 5.4.1 Body Protection 5.4.2 Eye and Face Protection 5.4.3 Hand Protection 5.4.4 Foot Protection 5.4.5 Respiratory Protection, Respirators, and Face Masks 5.5 Labels and Signs 5.6 Facilities, Laboratory Equipment, and Related Practices 5.6.1 Cleanable Surfaces and Furnishings 5.6.2 Doors and Windows 5.6.3 Plumbing Systems and Equipment 5.6.3.1 Sinks and Handwashing 5.6.3.2 Drains and Disposal 5.6.3.3 Water Systems and Backflow Prevention 5.6.3.4 Emergency Eyewashes and Showers 5.6.4 Ventilation and Hoods 5.6.4.1 Room Ventilation 5.6.4.2 Hoods and Biosafety Cabinets 5.6.5 Food Facilities and Eating 5.6.6 Laboratory Tools and Equipment 5.6.6.1 Sharps 5.6.6.2 Centrifuges 5.6.6.3 Waste Containers 5.6.6.4 Equipment Connected to Building Vacuum Systems 5.7 Decontamination, Waste, and Decommissioning 5.7.1 Decontamination Processes and Antimicrobials 5.7.2 Surface and Equipment Decontamination 5.7.3 Waste Decontamination and Disposal 5.7.4 Laboratory and Equipment Decommissioning and Moves 5.8 Access and Security 5.9 Pest Management 5.10 Incident, Accident, and Emergency Response 5.10.1 General Incident Response and Reporting 5.10.2 Worker Exposure, Injury, or Illness 5.10.3 Biological Spills and Cleanup 5.10.4 Additional Biosafety Incident Reporting 5.11 Procurement, Transportation, and Transfer 5.11.1 Procurement 5.11.2 Transportation and Shipping 5.11.3 Import, Export, and Transfer Restrictions 6.0 Assessment and Improvement Printed copies are not official versions of this manual. Before using the printed copy, verify that it is the most current version. 3
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