Croatian Veterinary Institute (pdf) - SVA

CROATIAN VETERINARY
INSTITUTE
VPH dept.
Laboratory for Food Microbiology
Andrea Humski, Ph.D, DVM
Marina Mikulić, DVM
CROATIAN VETERINARY
INSTITUTE

about CVI...
Established in 1933.
Leading national diagnostic, research and scientific
institution in the field of veterinary medicine
-Ministry of Agriculture, Fishery and Rural Development
(MPRRR)
-Ministry of Science, Education and Sports (MZOS)
CROATIAN VETERINARY
INSTITUTE

AREAS OF OPERATION
1. DIAGNOSTIC AND ANALYTICAL
ACTIVITIES
Preparedness, field and laboratory diagnostics, profilaxis,
surveillance and monitoring, data analysis, planing and
proposal of preventive actions, reference and scientific
advisory functions in control of:
• Infectious diseases
• Zoonoses
• Food and feed safety
• Veterinary drugs and residues
CROATIAN VETERINARY
INSTITUTE

2. SCIENTIFIC AND RESEARCH ACTIVITIES
Performed within the framework of The National scientific
programme
Executed through the several scientific research projects
financially supported by Ministry of science, education and
sport
CROATIAN VETERINARY
INSTITUTE

CVI laboratory network
...employees
total no. – 238
• PhD – 34
• Msc – 13
• Sci. advisor – 7
• Senior sci. associate - 10
• Sci. associate – 11
CROATIAN VETERINARY
INSTITUTE

CP
Križevci
Vinkovci
CROATIAN VETERINARY
INSTITUTE

CVI Zagreb
I. VPH Dept.
I.1. Laboratory for food microbiology
I.2. Laboratory for residue control
I.3. Laboratory for feed microbiology
I.4. Laboratory for analytical chemistry
I.5. Laboratory for veterinary medicinal products
CROATIAN VETERINARY
INSTITUTE

LABORATORY FOR FOOD MICROBIOLOGY, Zagreb
• microbiological control of food: official control (import/export),
on request (domestic production/market)
• monitoring programmes
– microbiological contamination of meat in slaughterhouses
– microbiology food safety of products- veterinary
inspection on border posts
• research and scientific work
CROATIAN VETERINARY
INSTITUTE

LABORATORY FOR FOOD MICROBIOLOGY
ACCREDITATION CERTIFICATE
Microbiological parameter/method
1 Salmonella spp.
HRN EN ISO 6579:2003 / cor 2004
2 Salmonella spp. TECRA VIA – Screening method
AOAC Official Method 989.14;998.09
3 Listeria monocytogenes – Part 1.: Detection method
HRN EN ISO 11290 - 1:1999
4 Listeria monocytogenes – Part 2.: Enumeration method
HRN EN ISO 11290 - 2: 1999
5 Listeria spp. TECRA VIA – Screening method
AOAC Official Method 995.22;2002.09
6 Enumeration of microorganisms - CCT at 30°C
HRN ISO 4833:2003
7 Escherichia coli – Part 2.:Colony count technique at 44 °C
HRN ISO 16649-2:2001
8 Escherichia coli – Part 3.: MPN method
ISO/TS 16649-3:2005
9 Escherichia coli O157
HRN EN ISO 16654:2003
10 E. coli O157. TECRA VIA – screening method
AOAC –RI, L.NO.001101
11 Enterobacteriaceae –Part 1.: MPN method
ISO 21528-1:2004
12 Enterobacteriaceae – Part 2.: Colony count
technique
ISO 21528-2:2004
13 Staphylococcus aureus and other species – Part 1.:
Technique using Baird-Parker medium
HRN EN ISO 6888-1:2004
14 Sulfite-reducing bacteria
HRN ISO 15213:2004
15 Clostridium perfringens - Colony count technique
HRN EN ISO 7937: 2005
16 Campylobacter spp. – Part 1. Detection method
ISO 10272-1:2006
17 Campylobacter spp. – Part 2. Enumeration method
ISO 10272-2:2006
18 Campylobacter spp. TECRA VIA –
Screening method
CROATIAN VETERINARY
INSTITUTE

External Quality Assesment/Proficiency Testing
Health Protection Agency, London, UK (since 2005.)
Parameter
No. of tested samples
Salmonella spp. 15
Listeria monocytogenes / E 8
Listeria monocytogenes / D 4
E. coli, MPN 9
E. coli / E 2
E. coli O157 3
ACC 11
Campylobacter spp. 6
Coag. + Staphylococcus / E 5
Staph. aureus enterotoxin 2
Cl. perfringens / E 3
Enterobacteriaceae / E 3
CROATIAN VETERINARY
INSTITUTE

Present and future activities….
CROATIAN VETERINARY
INSTITUTE

Zoonoses in humans 2005 - 2008
Disease 2005
Cases Deaths Rate per 100,000 population Rank
Salmonellosis 5619 0 124.9804 1
Leptospirosis 126 0 2.8026 2
Q fever 40 0 0.8897 3
Toxoplasmosis 39 0 0.8675 4
Trichinellosis 27 0 0.6005 5
Disease 2006 Cases Deaths Rate per 100,000 population Rank
Salmonellosis 4734 1 105.2958 1
Trichinellosis 45 0 1.0009 2
Leptospirosis 39 0 0.8675 3
Toxoplasmosis 31 0 0.6895 4
Q fever 28 0 0.6228 5
Disease 2007 Cases Deaths Rate per 100,000 population Rank
Salmonellosis 3331 0 74.0897 1
Campylobacteriosis 141 0 3.1362 2
Leptospirosis 63 1 1.4013 3
Q fever 43 0 0.9564 4
Toxoplasmosis 31 0 0.6895 5
Disease 2008 Cases Deaths Rate per 100,000 population Rank
Salmonellosis 3664 0 81.4964 1
Campylobacteriosis 1294 0 28.7818 2
Q fever 41 0 0.9119 3
Brucellosis 25 0 0.5561 4
CROATIAN VETERINARY
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Toxoplasmosis 24 0 0.5338 5

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Salmonellosis
Campylobacteriosis
2005 2006 2007 2008
CROATIAN VETERINARY
INSTITUTE

1. Monitoring programme - Ordinance on conduction of veterinary inspection
and control of animal products in traffic across the border of the Republic of
Croatia (O.G. 132/08) / Ordinance on the document for registration of
veterinary health inspection of shipments of animal origin entering the
Republic of Croatia (O.G.129/06 and 75/07) Annex II
2. Baseline study acc. to Commission Decision 2007/516/EC: Campylobacter
and Salmonella prevalence in broiler flocks (carcasses + caecal content)
CROATIAN VETERINARY
INSTITUTE

THANK YOU FOR ATTENTION
CROATIAN VETERINARY
INSTITUTE