2011 Key Products Guide - Fortune Scientific Co., Ltd.

2011 Key Products Guide 

HPLC/UHPLC 

GC 

Sample Preparation 

Preparative HPLC 

Chiral Chromatography 

GPC/SEC 

GFC/SEC 

Bioseparations

Thank You 

To Our Customers Heroes, 

You are such a huge part of our journey, so it’s only appropriate that 

we begin by taking a moment to say, “THANK YOU”. 

Why? Because you inspire us. 

We applaud you for making the world better each day. Thank you for helping 

people, discovering cures, inventing new technologies, dedicating your lives to 

research, advancing science through innovation, thinking beyond limitations, and 

for protecting our food, our health, our planet, and so much more. 

We admire you because you spend every day doing your best to make a better 

life for all of us, our families, and our world. For this, we want to support you in 

every way we can. 

Here is to a year of imagining (and achieving) the impossible, together. 

The Phenomenex Family 

www.phenomenex.com 

Phenomenex products are available worldwide. 

For the distributor in your country, contact Phenomenex USA, 

International Department at international@phenomenex.com 

BR83481110_I_2

Come visit the brand NEW www.phenomenex.com 

Thousands of resources available for you: 

• Chromatography Tools 

• Applications 

• Technical Support 

• Technical Notes 

• Sample Request 

• Detailed Product Information 

Request Your Copy of the 

2011 Phenomenex Chromatography Catalog TODAY! 

www.phenomenex.com/catalog 

• Includes information on over 200 brands for HPLC, GC and sample preparation 

• Comprehensive tips for method development and troubleshooting 

• Contains hundreds of HPLC, GC, and sample preparation applications 

Terms and Conditions 

Offers and discounts must be specified at time of order and cannot be combined with any other discount or promotional items. The information, offers, and 

descriptions in this brochure are subject to change without notice. Subject to Phenomenex Standard Terms and Conditions, which may be viewed at 

www.phenomenex.com/termsandconditions 

Trademarks 

Gemini, Jupiter, Luna, Lux, and Strata are registered trademarks of Phenomenex, Inc. Arylene Matrix Technology, Axia, BioSep, Glass Infusion, Impact, Inferno, 

Kinetex, KrudKatcher, MercuryMS, MidBore, Phenex, Phenogel, pHLC, Rezex, SecurityGuard, Strata-X, Synergi, TWIN-NX, WAXPLUS and Zebron are trademarks 

of Phenomenex, Inc. Aminex, Bio-Rad, and Bio-Sil are registered trademarks of Bio-Rad Laboratories. CHIRALCEL, CHIRALPAK, AD, OD-H, and OJ-H are 

registered trademarks of DAICEL Chemical Industries, Ltd. Cyrolite is a registered trademark of CY/RO Industries. HYPERSIL is a registered trademark of Thermo 

Hypersil-Keystone, Inc. Interaction is a registered trademark of Transgenomic/Cetac. J&W is a registered trademark of J&W. Nucleosil is a registered trademark of 

Macherey-Nagel. PLgel is a trademark of Polymer Laboratories, Ltd. ACQUITY, UPLC, Styragel, XTerra, and Waters are registered trademarks of Waters Corporation. 

BioSuite, OBD, Sugar-Pak, µStyragel, and Ultrastyragel are trademarks of Waters Corporation. PEEK is a registered trademark of ICI Americas, Inc. Teflon is a 

registered trademark of E.I. du Pont de Nemours and Co. SRT is a registered trademark of Sepax. SUPELCOGEL is a trademark of Supelco. TSKgel and TSK-GEL 

are registered trademarks of Tosoh Corporation. VYDAC is a registered trademark of Alltech Associates, Inc. Agilent Technologies and ZORBAX are registered 

trademarks of Agilent Technologies. Rtx, MXT, Rxi, Stx, XTI and StabilWax are registered trademarks of Restek. All other trademarks belong to their respective 

owners. Phenomenex, Inc. is in no way affiliated with the above companies. 

Disclaimer 

Strata-X and AXIA are patented by Phenomenex, Inc. 

© 2011 Phenomenex, Inc. All rights reserved. 

www.phenomenex.com 

international@phenomenex.com

Phenomenex ® 

Contact Phenomenex for all of your chromatography consumable needs. In addition to the products 

featured in this guide, Phenomenex manufactures or supplies many other HPLC, GC & SPE columns and 

accessories. 

Accessories 

• General Laboratory Consumables 

– Autosampler Vials – Filtration System 

– Centrifugal Filters – Syringe Filters 

– Filter Membranes – Syringes 

• GC 

– Cool-Lock Nut – Liners 

– Ferrules – Septa 

– Inlet Base Seals – Traps / Purifiers 

• HPLC 

– Column Heaters – Pipettes 

– Column Selectors – Rheodyne ® Sample Injectors 

– Fluid Processors – Solvent Degasser 

– Mobile Phase Accessories – Solvent Recycler 

Bulk Media 

GC Capillary Columns 

• Quadrex 

• SGE 

• Zebron 

HPLC Columns 

Aqua 

Gemini ® 

Asahipak ® 

Axia Packed Columns 

Beckman Coulter Ultrasphere ® 

BioSep 

Bondclone 

Capcell Pak ® 

Ceramospher ® and Chiral CD-Ph 

Chiral Method Development Kits 

Chirex ® 

Chromolith ® 

Clarity ® 

Columbus 

Cosmosil 

Curosil / Taxol ® 

Develosil ® 

EnviroSep 

EZ:faast 

Sample Preparation / SPE 

• Accessories 

• Strata ® Silica SPE Sorbents 

• Strata -X Polymeric SPE Sorbents 

• 96-Well Plates 

• Tubes 

• Vacuum Manifolds 

Gemini ® -NX 

Hamilton ® 

Hypercarb 

HyperClone 

HYPERSIL ® 

HYPERSIL ® BDS 

IB-Sil 

Inertsil ® 

Jupiter ® 

Kinetex 

Kingsorb 

Kromasil ® Chiral 

LiChrosorb ® 

LiChrospher ® 

Luna ® 

Lux 

Maxsil 

Merck KGaA 

Shodex ® 

Nucleosil ® 

Shodex ® Chiral 

Nucleosil ® Chiral 

SphereClone 

Onyx 

Spherex 

Partisil ® 

Spherisorb ® 

PartiSphere ® 

STAR-ION A300 

Phenogel 

Sumichiral OA Chiral 

PhenoSphere 

Synergi 

PhenoSphere -NEXT Ultracarb 

Polymer Labs 

Ultrasphere ® 

PolymerX 

Ultremex 

PolySep -GFC-P 

Ultron ® ES Chiral 

PREP Columns 

VYDAC ® 

PrimeSphere 

Whatman ® 

Prodigy 

ZORBAX ® 

Rezex 

SecurityGuard 

Selectosil 

If you are not completely satisfied with 

any of the products featured in this 

brochure, return within 45 days for a 

FULL Refund! 

All trademarks are properties of their respective owners. 

international@phenomenex.com 

www.phenomenex.com

Sample Filtration 

SPE (Solid Phase Extraction) 

P.P. 2-3 

P.P. 4-8 

Phenex 

Syringe Filters 

Strata -X 

GC Columns 

P.P. 9-21 

Zebron GC Columns 


Kinetex 


P.P. 26-35 

HPLC 

Chiral Analysis 

Lux ® 

P.P. 42-44 

Rezex 

Carbohydrate 

Organic 

Acid Analyis 

P.P. 52-54 

P.P. 55-56 

GPC 

Phenogel 

Protein/Peptide Analysis & Purification 

Jupiter ® 300 RP-HPLC 

Jupiter Proteo RP-HPLC 

BioSep GFC/SEC 

P.P. 45-51 

international@phenomenex.com 

www.phenomenex.com


Phenex Syringe Filters 

For sample and solvent filtration prior to 

chromatography 

• Rapid filtration of HPLC and GC samples prior to analysis 

• Particulate, PVC, and extractable-free filters 

• Less system downtime 

• More consistent, reproducible results 

• Increased column lifetime 

Syringe Filter Selection Guide 

Phenex Offers: 

• Maximum chemical compatibility • 100 % integrity tested 

• Minimum extractables 

• Low hold-up volume 

• Excellent flow rate 

• Low protein adsorption 

• High total throughput 

• Bi-directional use 

• Certified quality 

1. Choose filter diameter based on sample volume 

4 mm Diameter 15 - 17 mm Diameter 25 - 28 mm Diameter 

≤ 2 mL Sample Volume 2 - 10 mL Sample Volume 10 - 100 mL Sample Volume 

2. Choose a pore size based on the nature of your sample and chromatographic method 

Sample Description 

Recommended Filter Pore Size 

General aqueous or mixed organic samples prior to HPLC analysis with column packed with > 3 µm particles. 

General clarification of GC, SFC, CE, and GPC samples. 0.45 µm 

Viscous samples or samples containing high levels of particulate matter. 

General aqueous or mixed organic samples prior to HPLC analysis with columns packed with < 3 µm particles. 

Removal of fine particulate matter prior to GC, SFC, CE, and GPC samples. 0.20 µm 

Gas samples prior to GC. Liquid samples prior to UHPLC or LC/MS. Other particulate-sensitive methods. 

Viscous samples such as serum, plasma or other biological matrices. Solutions with high particulate load such 

as some environmental or food and beverage applications. 

Glass Fiber Filter 

with 0.45 µm filter 

3. Choose a filter membrane according to the characteristics of your sample and filtering objective 

Membrane Type 

RC 

(Regenerated Cellulose) 

PTFE, Teflon ® 

(Polytetrafluoroethylene) 

PES 

(Polyethersulfone) 

NY 

(Nylon) 

CA 

(Cellulose Acetate) 

GF 

(Glass Fiber) 

Recommended Uses 

Hydrophilic Regenerated Cellulose filter membranes are compatible with a very broad range of aqueous and mixed-organic solutions, 

making them one of the most universal filter materials used prior to chromatography. Phenex-RC filters also exhibit fast-flow and 

ultra-low protein and non-specific binding characteristics. Due to the beneficial material characteristics, Phenex-RC membranes are 

broadly recommended as an excellent general purpose/high-performance sample filter for most applications. 

PTFE is an inherently hydrophobic membrane excellent for filtration of organic-based, highly acidic or basic samples and solvents. 

Widely used in chromatography, it is especially well suited for the clarification of non-aqueous samples. Although this membrane is 

hydrophobic, it can be made hydrophilic by wetting the membrane with alcohol and then flushing with deionized water. 

Polyethersulfone membranes exhibit very fast-flow and ultra-low protein binding characteristics and are ideally suited for use in many 

life science clarification applications. Phenex-PES membranes typically offer better chemical resistance than cellulose acetate and 

are broadly recommended for filtering critical biological samples, tissue culture media, additives and buffers. 

Nylon has inherent hydrophilic characteristics and works well for filtration of many aqueous and mixed-organic samples. For applications 

that require low protein or non-specific binding characteristics, Phenomenex recommends Phenex-RC (Regenerated Cellulose) 

filters. 

Cellulose Acetate membranes exhibit ultra-low protein binding and are broadly used in the filtration of biological samples. In 

combination with a glass pre-filter (Phenex-GF/CA), this membrane is excellent for filtration of tissue culture media, general biological 

sample filtration and clarification. 

Phenex-GF (Glass Fiber) filters are made of inert borosilicate glass and have a nominal 1.2 µm pore size. They are commonly used 

with highly viscous samples or samples that contain high concentrations of particulate matter (e.g., food analysis, biological samples, 

soil samples, fermentation broth samples, removal of yeasts, molds, etc.). Glass Fiber filters can be used alone or in series with other 

Phenex filter membranes such as the 0.45 µm pore Phenex-RC filter to reduce clogging of the membrane and optimize flow. 

Phenex is a trademark of Phenomenex, Inc. 

2 www.phenomenex.com international@phenomenex.com 

Phenomenex


Syringe Filters (cont’d) 

Syringe Filter Applications and Recommended Membranes 

Application / Sample 

Recommended 

Filter 

First 

Alternative 

Second 

Alternative 

HPLC and GC Sample Prep RC PTFE PES 

Aggressive or Pure Organic Solvents PTFE RC NY 

Protein Analysis / Biological Samples PES RC GF/CA 

High Particulate Loads GF/NY GF + RC PTFE 

Environmental Methods GF/NY RC PTFE 

Food and Beverage GF/NY RC PTFE 

Clinical / Toxicology RC PES NY 

Dissolution Testing GF/NY RC PTFE 

Ion Chromatography RC PES PTFE 

Trace Metals (ICP-MS, AAS) RC PES NY 

Capillary Electrophoresis (CE) RC PES NY 

Tissue Cultures, Media, Buffers GF/CA PES RC 

Ordering Information 

Phenex Syringe Filters 

4 mm Diameter 

for ≤ 2 mL sample volumes 

15 - 17 mm Diameter 

for 2 – 10 mL sample volumes 

Tip: Try a Sample Pack! 

Request yours today by phone or visit 

www.phenomenex.com/sample 

For high load and particulate-laden samples 

you may consider placing a Glass Fiber (GF) 

pre-filter in line with the membrane syringe 

filter of your choice or choose a filter with an 

integrated glass fiber membrane. 

25 - 28 mm Diameter 

for 10 – 100 mL sample volumes 

Membrane Type/Size Part No. Unit Part No. Unit Part No. Unit 

0.20 µm 

Phenex-RC 

AF0-3203-12 100/pk AF0-2203-12 100/pk AF0-8203-12 5 100/pk 


AF0-3203-52 500/pk AF0-2203-52 500/Pk AF0-8203-52 5 500/pk 

Phenex-PES 3 

— — — — AF0-8208-12 7 100/pk 


— — — — AF0-8208-52 7 500/pk 

Phenex-PTFE 6 

AF0-3202-12 100/pk AF0-2202-12 100/pk AF0-1202-12 100/pk 


AF0-3202-52 500/pk AF0-2202-52 500/pk AF0-1202-52 500/pk 

Phenex-NY 

AF3-3207-12 100/pk AF0-2207-12 100/pk AF0-1207-12 100/pk 

(Nylon) 

AF3-3207-52 500/pk AF0-2207-52 500/pk AF0-1207-52 500/pk 

Phenex-GF/NY 

An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a Nylon AF0-1A47-12 

100/pk 

(Glass Fiber/Nylon) 

(NY) membrane. Excellent for filtration of particle-laden samples, such as foods and beverages, 

environmental, biofuels, and dissolution samples. Use less hand pressure to filter even the most 

AF0-1A47-52 

500/pk 

difficult samples. Outlet connection is luer-lock. 

Phenex-GF/CA 2,3,4 

(Glass Fiber/Cellulose Acetate) 

0.45 µm 

Phenex-RC 


Phenex-PES 3 


Phenex-PTFE 6 


Phenex-NY 

(Nylon) 

Phenex-GF/NY 

(Glass Fiber/Nylon) 

Phenex-GF/CA 2,3,4 

(Glass Fiber/Cellulose Acetate) 

1.20 µm 

Phenex-GF 2,3 

(Glass Fiber) 

An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA 

membrane. Excellent for filtration of tissue culture media, general biological sample filtration 

and clarification. Outlet connection is luer lock. 

AF0-8A09-12 7 

AF0-8A09-52 7 

100/pk 

500/pk 

AF0-3103-12 100/pk AF0-2103-12 100/pk AF0-8103-12 5 100/pk 

AF0-3103-52 500/pk AF0-2103-52 500/Pk AF0-8103-52 5 500/pk 

— — AF2-5108-12 1 100/pk AF0-8108-12 7 100/pk 

— — — — AF0-8108-52 7 500/pk 

AF0-3102-12 100/pk AF0-2102-12 100/pk AF0-1102-12 100/pk 

AF0-3102-52 500/pk AF0-2102-52 500/pk AF0-1102-52 500/pk 

AF3-3107-12 100/pk AF0-2107-12 100/pk AF0-1107-12 100/pk 

AF3-3107-52 500/pk AF0-2107-52 500/pk AF0-1107-52 500/pk 

An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a Nylon AF0-1B47-12 

100/pk 

(NY) membrane. Excellent for filtration of particle-laden samples, such as foods and beverages, 

AF0-1B47-52 

environmental, biofuels, and dissolution samples. Use less hand pressure to filter even the most 

500/pk 

difficult samples. Outlet connection is luer-lock. 

An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA AF0-8B09-12 7 

100/pk 

membrane. Excellent for filtration of tissue culture media, general biological sample filtration 

and clarification. Outlet connection is luer lock. 

AF0-8B09-52 7 

500/pk 

Prefiltration of heavily contaminated or highly viscous samples. When used in-series preceding 

a membrane filter, clogging of the membrane filter is prevented and sample clean up is 

optimized. Outlet connection is luer lock. 

AF0-8515-12 7 

AF0-8515-52 7 

100/pk 

500/pk 

1. 17 mm diameter. 

2. Glass fiber filters are 28 mm diameter and made of borosilicate. 

They will remove 90 % of all particles >1.2 µm. 

3. Housing material is methacrylate butadiene styrene (MBS) polymerisate. 

Also known as Cyrolite ® . 

4. Cellulose acetate is surfactant-free. 


6. Hydrophobic membrane. Can be made hydrophilic by pre-wetting 

with IPA. 


8. Additional dimensions and membrane types are available. Please 

contact your local Phenomenex technical consultant or distributor 

for availability or assistance. 

9. Larger quantity purchases at significant savings are available. 

Above syringe filters are non-sterile. Housing is made of 

medical-grade polypropylene (PP), and offer luer lock inlet/slip 

outlet connections, unless otherwise indicated. 

Phenomenex international@phenomenex.com www.phenomenex.com 

3


Strata -X 

Functionalized Polymeric SPE 


Tools and Solutions 

p. 5 

Strata-X 

Functionalized Polymeric SPE 

p. 6-7 

Strata ® 

Traditional Silica SPE 

p. 8 

Strata Impact 

Rapid Protein Precipitation 

p. 8 


Phenomenex


Sample Preparation Techniques 

Strata -X and Strata ® SPE Strata ® Impact Protein Precipitation Plates 

No Method Development Required 

X 

Increase Column Lifetime X X 

Superior Protein Removal X X 

Superior Lipid Removal 

X 

Removes Other Matrix Contaminants X 

Specific Selectivity for your Target Analytes X 

Concentrate Sample 

X 

De-Salt Sample 

X 

Perform Solvent Switch 

X 

Available Formats 

96-Well Plates Syringe Tubes 

Giga Tubes 

(12 mL - 150 mL) 

On-line Extraction 

Cartridge 

Strata-X Functionalized Polymeric SPE X X X X X 

Strata Traditional SPE X X X X X 

Strata Impact Protein Precipitation Plates X 

Bulk Sorbent 

Strata is a registered trademark of Phenomenex, Inc. 

Strata-X, Giga, Sepra, and Impact are trademarks of Phenomenex, Inc. 

Strata-X is patented by Phenomenex, Inc. 

Sample Preparation Solutions 

More than just a product, Strata -X and Strata ® sample preparation products 

are supported by customer and technical resources that are dedicated to your 

satisfaction and success. 

Sample Preparation Specialist 

There is a Sample Preparation Specialist dedicated to your company. 

Contact your Sample Preparation Specialist for: 

• Industry specific applications and technical resources 

• Product support 

• Method development assistance 

• Any question at all 

SPE Method Development Tool 

Solid Phase Extraction (SPE) method development tool allows you to quickly 

take advantage of the sample cleanup benefits SPE offers by: 

• Developing a complete SPE method around your 

target compound 

• Walking you through method troubleshooting 

• Offering method optimization steps for the highest 

yields 

Access your 

Sample Preparation Specialist: 

www.phenomenex.com/info/spehelp 

SPE Method Development Tool: 

www.phenomenex.com/info/mdtool 


5

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Strata -X Functionalized Polymeric SPE 


Compound of Interest 

Neutral 

Weak Acid 

Strong Acid 

Weak Base 

Try this Strata-X 

SPE Sorbent: 

X 

X-A 

X-AW 

X-C 

Modified pyrrolidone ligand offers multiple 

mechanisms of retention Ṅ . 

)n 

)n 

)n 

NH 

Ṅ . O 

)n 

S 

Di-methyl butyl quaternary O amine ligand 

offers a strong )n anionic mechanism 

O H + of 

retention n 

S O - 

) 

)n 

)n 

CH3 

OCH3 CI - 

Di-amino ligand offers a weak anionic 

mechanism of retention 

)n 

)n 

O 

OH 

O 

NH O 

2 

)n 

Sulfonic Acid ligand offers Ṅ . O 

a strong 

cationic mechanism of retention Ṅ . 

)n 

)n 

N + 

NH 

O 

O 

OH 

O 

O - H + 

NH 2 

H + 

S O O - H + 

O S O - 

Material Characteristics: 

Particle Size (µm) 33 

Pore Size (Å) 85 

Surface Area (m 2 /g) 800 

pH Stability 1-14 





Ionic Capacity 0.30 meq/g 

pK a 

~ 14 






pK a 

~ 9 





Ionic Capacity 1 meq/g 

pK a 

~ 0 

Strong Base 

All Strata-X phases (X, X-A, X-AW, X-C and X-CW) are also available NH in NH 

)n 

a 2 large particle / large pore size formats 

Sample Type 

• Large Target Analyte (> 10kDa) 

• Large Volume Samples 

• Viscous Samples 

X-CW 

Carboxylic Acid ligand offers O a weak 

cationic mechanism of retention 

)n 

)n 

)n 

Use Strata-XL 

NH 

Phase: 

XL, XL-A, XL-AW, 

XL-C or XL-CW 

O 

O 

OH 

OH 

NH 2 






pK a 

~ 4.5 

Material Characteristics 





Determine the Correct Sorbent Mass 

Strata -X, X-C, X-CW, 

X-A, X-AW, XL, XL-C, 

XL-CW, XL-A, XL-AW 

Sorbent Mass 

(in tube) Plasma/Serum Urine 

Filtered Tissue 

Homogenates 

Water 

(particulate-free) 

Water 

(particulate-laden) 

10 mg 100 µL 250 µL 10 mg 2 mL 1 mL 

30 mg 250 µL 1 mL 50 mg 10 mL 5 mL 

60 mg 500 µL 2 mL 100 mg 25 mL 10 mL 

100 mg 1 mL 4 mL 150 mg 50 mL 25 mL 

200 mg 2 mL 8 mL 300 mg 100 mL 50 mL 

500 mg 5 mL 20 mL 500 mg 500 mL 100 mL 

Determine the Correct Sorbent Wash and Elution Volumes 

10 mg 30 mg 60 mg 100 mg 150 mg 200 mg 500 mg 1 g 2 g 5 g 10 g 

Sorbent Mass 

Practical Minimum Wash and 

Elution Volume 4 bed volumes 100 µL 300 µL 600 µL 1 mL 1.5 mL 2 mL 5 mL 10 mL 20 mL 50 mL 100 mL 

Recommended Wash and 

Elution Volume 8 bed volumes 

200 µL 600 µL 1.2 mL 2 mL 3 mL 4 mL 10 mL 20 mL 40 mL 100 mL 200 mL 


Phenomenex


Strata -X Functionalized Polymeric SPE 

Strata -X Compound-Directed Phase Selection 

Compound-Directed Phase Selection 

Strata -X 

Strata -XL 

Analyte pK a 

0 1 2 3 4 5 6 Neutral 8 9 10 11 12 13 14 

Neutral Analyte or Analyte with log P > -0.5 

Modified N-Vinylpyrrolidone functional group results in multiple mechanisms of retention for the widest 

range of selectivity. Strong retention of neutral compounds allows for aggressive washes for increased 

sensitivity and method reproducibility. 

Strata -X-A 

Strata -XL-A 

Strata -X-AW 

Strata -XL-AW 

Strata -X-C 

Strata -XL-C 

Strata -X-CW 

Strata -XL-CW 

Acidic Analyte pK a ≥ 2 

Dimethyl butyl quaternary amine functional group 

provides strong retention of weakly acidic analytes 

and allows for 100 % organic washes, increasing 


Acidic Analyte pK a ≤ 5 

Di-amino functional group is highly selective for charged 

acidic compounds and allows for 100 % organic washes, 

increasing sensitivity and method reproducibility. 

Basic Analyte pK a ≤ 10.5 

Sulfonic acid functional group is highly selective for weakly basic 

compounds and allows for 100 % organic washes, increasing 


Basic Analyte pK a ≥ 8 

Carboxylic acid functional group allows for reversible binding 

of strong bases and quaternary amines. High selectivity for all 

basic compounds allows for 100 % organic washes, increasing 


Traditional Polypropylene Tubes 

1 mL (100/box) 6 mL (30/box) 

Phase 10 mg 30 mg 60 mg 100 mg 200 mg 500 mg 1 g 

Strata-X-AW – 8B-S038-TAK – 8B-S038-ECH 8B-S038-FCH 8B-S038-HCH – 

Strata-X-A – 8B-S123-TAK – 8B-S123-ECH 8B-S123-FCH 8B-S123-HCH – 

Strata-X 8B-S100-AAK 8B-S100-TAK ‡ 8B-S100-UAK 8B-S100-ECH 8B-S100-FCH 8B-S100-HCH – 

Strata-X-C 8B-S029-AAK 8B-S029-TAK ‡ – 8B-S029-ECH 8B-S029-FCH 8B-S029-HCH – 

Strata-X-CW – 8B-S035-TAK – 8B-S035-ECH 8B-S035-FCH 8B-S035-HCH 8B-S035-JCH 

Strata-XL-AW – 8B-S051-TAK – 8B-S051-ECH 8B-S051-FCH 8B-S051-HCH – 

Strata-XL-A – 8B-S053-TAK – 8B-S053-ECH 8B-S053-FCH 8B-S053-HCH – 

Strata-XL – 8B-S043-TAK – 8B-S043-ECH 8B-S043-FCH 8B-S043-HCH – 

Strata-XL-C – 8B-S044-TAK – 8B-S044-ECH 8B-S044-FCH 8B-S044-HCH – 

Strata-XL-CW – 8B-S052-TAK – 8B-S052-ECH 8B-S052-FCH 8B-S052-HCH – 

3 mL (50/box) 

Phase 30 mg 60 mg 100 mg 200 mg 500 mg 

Strata-X-AW 8B-S038-TBJ 8B-S038-UBJ 8B-S038-EBJ 8B-S038-FBJ 8B-S038-HBJ 

Strata-X-A 8B-S123-TBJ 8B-S123-UBJ 8B-S123-EBJ 8B-S123-FBJ 8B-S123-HBJ 

Strata-X 8B-S100-TBJ 8B-S100-UBJ ‡ 8B-S100-EBJ 8B-S100-FBJ † 8B-S100-HBJ 

Strata-X-C 8B-S029-TBJ 8B-S029-UBJ ‡ 8B-S029-EBJ 8B-S029-FBJ 8B-S029-HBJ 

Strata-X-CW 8B-S035-TBJ 8B-S035-UBJ – 8B-S035-FBJ 8B-S035-HBJ 

Strata-XL-AW – 8B-S051-UBJ 8B-S051-EBJ 8B-S051-FBJ 8B-S051-HBJ 

Strata-XL-A – 8B-S053-UBJ 8B-S053-EBJ 8B-S053-FBJ 8B-S053-HBJ 

Strata-XL – 8B-S043-UBJ 8B-S043-EBJ 8B-S043-FBJ 8B-S043-HBJ 

Strata-XL-C – 8B-S044-UBJ 8B-S044-EBJ 8B-S044-FBJ 8B-S044-HBJ 

Strata-XL-CW – 8B-S052-UBJ 8B-S052-EBJ 8B-S052-FBJ 8B-S052-HBJ 

On-Line Cartridges (1 ea)* 

Phase 

20 x 2.0 mm 

Strata-X-AW – 

Strata-X-A – 

Strata-X 

00M-S033-B0-CB 

Strata-X-C 00M-S048-B0-CB 

Strata-X-CW 00M-S036-B0-CB 

Strata-XL-AW – 

Strata-XL-A – 

Strata-XL – 

Strata-XL-C – 

Strata-XL-CW – 

*Requires 20 mm Cartridge Holder (CH0-5845) 

96-Well Plates, 2 mL (2/box) 

Phase 10 mg 30 mg 60 mg 

Strata-X-AW 8E-S038-AGB 8E-S038-TGB 8E-S038-UGB 

Strata-X-A 8E-S123-AGB 8E-S123-TGB 8E-S123-UGB 

Strata-X 8E-S100-AGB 8E-S100-TGB 8E-S100-UGB 

Strata-X-C 8E-S029-AGB 8E-S029-TGB 8E-S029-UGB 

Strata-X-CW 8E-S035-AGB 8E-S035-TGB 8E-S035-UGB 

Strata-XL-AW – 8E-S051-TGB – 

Strata-XL-A – 8E-S053-TGB – 

Strata-XL – 8E-S043-TGB – 

Strata-XL-C – 8E-S044-TGB – 

Strata-XL-CW – 8E-S052-TGB – 

12 mL (20/box) 20 mL (20/box) 60 mL (16/box) 150 mL (8/box) 

Phase 500 mg 1 g 2 g 1 g 2 g 5 g 5 g 10 g 20 g 50 g 

Strata-X-AW 8B-S038-HDG 8B-S038-JDG – 8B-S038-JEG 8B-S038-KEG – 8B-S038-LFF – – – 

Strata-X-A 8B-S123-HDG 8B-S123-JDG – 8B-S123-JEG 8B-S123-KEG – 8B-S123-LFF – – – 

Strata-X 8B-S100-HDG 8B-S100-JDG – 8B-S100-JEG 8B-S100-KEG – 8B-S100-LFF 8B-S100-MFF 8B-S100-VFF 8B-S100-YSN 

Strata-X-C 8B-S029-HDG 8B-S029-JDG – 8B-S029-JEG 8B-S029-KEG – 8B-S029-LFF 8B-S029-MFF 8B-S029-VFF – 

Strata-X-CW 8B-S035-HDG 8B-S035-JDG – 8B-S035-JEG 8B-S035-KEG – 8B-S035-LFF – – – 

Strata-XL-AW – – 8B-S051-KDG – 8B-S051-KEG 8B-S051-LEG 8B-S051-LFF 8B-S051-MFF 8B-S051-VFF – 

Strata-XL-A – – 8B-S053-KDG – 8B-S053-KEG 8B-S053-LEG 8B-S053-LFF 8B-S053-MFF – – 

Strata-XL – – 8B-S043-KDG – 8B-S043-KEG 8B-S043-LEG 8B-S043-LFF 8B-S043-MFF 8B-S043-VFF 8B-S043-YSN 

Strata-XL-C – – 8B-S044-KDG – 8B-S044-KEG 8B-S044-LEG 8B-S044-LFF 8B-S044-MFF 8B-S044-VFF – 

Strata-XL-CW – – 8B-S052-KDG – 8B-S052-KEG 8B-S052-LEG 8B-S052-LFF 8B-S052-MFF – – 

‡ Available in tabless 


7

Sample Preparation Strata ® 

Strata Traditional Solid Phase Extraction 

(SPE) Sorbents 

If you do not currently use polymeric SPE sorbents, contact us today for any of 

the traditional silica SPE sorbents seen in the table below. 

Determine the Correct Sorbent Mass 

Phases 

Reversed Phases Normal Phases Ion-Exchange Mixed-Mode Specialty 

C18-E CN SCX Screen-C GF FL-PR (Acids) 

C18-U NH 1 

WCK Screen-C EPH ( Extratable Petroleum (Hydrocarbon) 

C18-T Si-1 (Silica) SAK Screen-A AL-N Aluminum Neutral 

C8 ABW SCB-L 

Phenyl 

Eco-Screen 

Melamine, 

PAH 

Biological Sample Cleanup 

Strata ® Impact 

Rapid Protein Precipitation Without Centrifugation 

• Quickly cleanup sample by passing biological samples 

through the centrifugation-free protein precipitation plate 

• Increase sensitivity of your analysis by eliminating 

proteins which contribute to baseline noise 

• Increase reproducibility with the leak-free membrane, 

preventing premature sample breakthrough and 

incomplete protein precipitation 

Solvent 

Shielding 

Technology 

prevents 

leaks 

Leak 

occurs 

Standard 

Precipitation Plate 

Leak-free 

Strata Impact Plate 

Can retain acetonitrile with no leaks for 

greater than 20 minutes 


Strata Impact Precipitation Plates 

Part No. Description Unit/Box 

Strata Impact Precipitation Plates 

CE0-7565 Strata Impact Protein Precipitation, Square Well, 2 

Filter Plate, 2 mL 

Strata Impact Starter Kit for Protein Precipitation 

CE0-8201 Strata Impact Protein Precipitation Plate (2 ea) 

Collection Plate 2 mL (2 ea) 

Sealing Mat, Santoprene (AH0-8199) (2 ea) 

ea 

‡ 

When used with a liquid-handling instrument or automation, the pipette 

tip can aspirate sample followed by organic for in-tip precipitation initiation 

prior to application of the sample to the organic in the well. Vortexing is not 

necessary when in-tip precipitation is performed. 


Phenomenex


Zebron GC Columns 

GC Column Selection by Manufacturer p. 10 

GC Column Selection by Analyte/Compound p. 11 

Zebron ZB-1ms p. 12 

Zebron ZB-1HT Inferno p. 13 

Zebron ZB-5ms p. 14 

Zebron ZB-5HT Inferno p. 15 

Zebron ZB-624 p. 16 

Zebron ZB-WAXPLUS p. 17 

Zebron ZB-Multiresidue 1 and 2 p. 18-19 

Zebron ZB-Drug-1 p. 20 

Zebron ZB-1XT SimDist p. 21 


9

GC Column Selection 

By Manufacturer 

Composition 

100 % dimethylpolysiloxane 




5 %-phenyl- 

95 %-dimethylpolysiloxane 

5 %-phenyl- 


5 %-phenyl- 


5 %-phenyl-Arylene- 


35 %-phenyl- 


Phenomenex 

Zebron 

ZB-1 

Restek 

Rtx ® -1, 

Rtx-1PONA, 

Rtx-1 F&F 

Agilent 

Technologies 

(J&W) 

DB-1, 

DB-2887, 

DB-1 EVDX 

ZB-1ms Rtx-1MS DB-1ms 

Supelco 

SPB-1, SPB-1 TG, 

SE-30, MET-1, SPB- 

1 Sulfur, SPB-HAP 

MDN-1, 

Equity-1 

Agilent 

Technologies 

(HP) Alltech SGE 

HP-1, 

HP-101, 

HP-PONA, Ultra 1 

AT-1, 

AT-Sulfur, 

EC-1 

BP1, BP1-PONA, 

BPX1-SimD 

HP-1ms AT-1ms SolGel-1ms 

Varian 

(Chrompack) 

CP-Sil 5 CB 

CP-Sil 5 CB MS, 

VF-1ms 

ZB-1HT Inferno MXT ® -1 SimDist DB-1ht Petrocol 2887 CP-SimDist 

ZB-1XT SimDist MXT ® -1HT SimDist DB-HT Sim Dis 

ZB-5 Rtx-5 DB-5 

ZB-5MSi 

ZB-5HT Inferno 

ZB-5ms 

ZB-35 

MDN-5, SPB-5, 

PTE-5, SE-54, PTA- 

5, Equity-5, Sac-5 

HP-5, 

Ultra 2, 

HP-PAS-5 

Rtx-5MS, 

Rtx-5Amine, Rxi ® -5ms DB-5 MDN-5S HP-5ms, 

HP-5msi 

Stx ® -5HT, 

XTI ® -5HT 

Rtx-5Sil MS 

Rtx-35, 

Rtx-35MS 

AT-5, 

EC-5 

BP5, 

BPX5 

CP-SimDist 

UltiMetal 

CP-Sil 8 CB 

DB-5ht HT-5 VF-5ht 

DB-5ms, 

DB-5.625, 

DB-5ms EVDX 

DB-35, 

DB-35ms 

MDN-35, 

SPB-35, 

SPB-608 

HP-35, 

HP-35ms 

AT-35 

BPX35, 

BPX608 

VF-5ms, 

CP-Sil 8 CB MS 

OV 

OV-1 

OV-5 

OV-11 

Proprietary 

ZB-35HT Inferno 

50 %-phenyl- 


ZB-50 

6 %-cyanopropylphenyl-94 

%-dimethylpolysiloxane 

ZB-624 





ZB-1701P 

Rtx-50 

Rtx-1301, 

Rtx-624 

DB-17, DB- SP-2250, 

17HT, DB-17ms, SPB-17, 

DB-17 EVDX SPB-50 

DB-1301, 

DB-624, 

DB-VRX 

ZB-1701 Rtx-1701 DB-1701 

DB-1701P 

Polyethylene glycol ZB-WAX 

Rtx-WAX, Famewax, 

Stabilwax-DB 

DB-WAXetr 

DB-WAX, 

Polyethylene glycol ZB-WAXplus Stabilwax ® CAM 

Nitroterephthalic acid 

modified polyethylene ZB-FFAP Stabilwax-DA DB-FFAP 

glycol 

Proprietary 

MultiResidue-1 Rtx-CLPesticides, 

(MR-1) 

Stx-CLPesticides 

Proprietary 

MultiResidue-2 

(MR-2) 

Rtx-CLPesticides2, 

Stx-CLPesticides2 

SPB-1301, 

SPB-624 

SPB-1701, 

Equity-1701 

Met-Wax, 

Omegawax 

Supelcowax 10 

Nukol, 

SPB-1000 

HP-50+ AT-50 BPX50 CP-Sil 24 CB 

HP-VOC 

AT-624, 

AT-1301 

BP624 

CP-1301, 

CP-Select 624 CB 

OV- 624 

AT-1701 BP10 CP-Sil 19 CB OV -1701 

HP-INNOWax EC-Wax SolGel-WAX CP-Wax 57 CB 

HP-20M, 

Carbowax 20M 

HP-FFAP 

AT-Wax, 

AT-AquaWax 

AT-1000, 

EC-1000 

BP20 

BP21 

CP-Wax 52 CB 

CP-Wax 58 

(FFAP) CB, 

CP-FFAP CB 

Proprietary ZB-XLB Rtx-XLB DB-XLB MDN-12 VF-XMS 

Carbowax 

20M 

OV-351 

Proprietary 

Proprietary 

ZB-XLB-HT Inferno 

ZB-Drug-1 

Proprietary ZB-BAC1 Rtx-BAC1 DB-ALC1 

Proprietary ZB-BAC2 Rtx-BAC2 DB-ALC2 

Proprietary 

ZB-Bioethanol 

This section is, neither in terms of manufacturers nor in terms of their products, a complete list, and the accuracy of the data is not 

guaranteed. Small differences in dimensions or performance might be possible and slight adjustments to your application may be 

necessary. 

All trademarks are property of their respective owners. Phenomenex is in no way affiliated with 

companies listed above. 


Phenomenex

GC Column Selection 

Choose the best Zebron GC column based on your 

analyte/compound of interest. 

Your Analytes 

Non-Polar 

Mixed-Polarity 

Polar 

Semi-Volatile 

Volatile 

General Analytes 

Pesticides 

ZB-5ms 

ZB-MR-1 

ZB-XLB-HT Inferno 

ZB-35HT Inferno 

ZB-MR-1 

ZB-MR-2 

ZB-5ms 



ZB-624 

ZB-WAXPLUS 

ZB-WAX 

ZB-1* 

*Thick Film (≥1.00 µm) 

Semi-Volatile 

Volatile 

Base 

Neutral 

Acidic 

ZB-624 

ZB-WAXPLUS 

ZB-WAX 

ZB-WAX 


ZB-WAX 

ZB-50 



ZB-MR-2 

ZB-FFAP 


ZB-MR-2 

Volatile 

Semi-Volatile 

ZB-1* 

ZB-5* 

PLOT 

*Thick Film (≥1.00 µm) 

ZB-1ms 

ZB-5ms 

ZB-5MSi 




ZB-35 

ZB-1XT SimDist 


11

Zebron 


ZB-1ms 

• Very low bleed (MS Certified) especially suited to high 

sensitivity GC/MS 

• Extremely inert for active compounds such as drugs, 

pesticides, or acids and bases 

• Improved signal-to-noise ratio for better sensitivity and 

mass spectral integrity 

• Identical selectivity to the ZB-1 

Column Profile 

Low 

5 

Polarity 

Bleed 

Temperature Limits 

Stability 

High 

• Equivalent to USP Phase G2 

• Temperature Limits: -60 to 360/370 °C (Isothermal/TPGC) 

Applications 

• Amines 

• Acids 

• Diesel Fuel 

• Polychlorinated 

Biphenyls (EPA Method 

1668) 

• Drugs of abuse 

• Flavors & Fragrances 

• Pesticides 

Alternative to Any MS-Certified 100 % Dimethylpolysiloxane Phase: 

• DB-1ms 

• HP-1ms 

• AT-1ms 

• MDN-1 

• Rtx-1MS 

• VF-1ms 

• SOLGEL-1ms 

• Equity-1 

• CP-Sil 5 CB ms 

Lower Overall Column Activity 

Activity is a key measure of column quality. This is why Zebron 

ZB-1ms columns are aggressively tested to ensure full deactivation. 

Below is an example of the aggressive QC test mix we use, 

notice the low tailing for even the most active compounds like 

2-Ethylhexanoic Acid! 

Test Conditions for Zebron ZB-1ms 

1 

1 

2 

3 

4 

5 

6 7 

Column: Zebron ZB-1ms 

Dimensions: 30 meter x 0.25 mm x 0.25 µm 

Part No.: 7HG-G011-11 

Injection: Split Flow 100 mL/min @ 250 °C, 1.0 µL 

Carrier Gas: Hydrogen @ 1.18 mL/min (constant flow) 

Oven Program: 140 °C (Isothermal) 

Detector: FID @ 325 °C 

Sample: 1. Decane 

2. 2-Ethylhexanoic Acid 

3. 4-Chlorophenol 

4. Naphthalene 

5. Tridecane 

6. 1-Undecanol 

7. Dicyclohexylamine 

8. Pentadecane 

5 10 min 

8 

App ID 15545 


Zebron ZB-1ms GC Columns 

ID(mm) df(µm) Temp. Limits °C Part No. 

10-Meter 

0.10 0.10 -60 to 360/370 7CB-G011-02 

0.18 0.18 -60 to 360/370 7CD-G011-08 

12-Meter 

0.20 0.33 -60 to 360/370 7DE-G011-14 

15-Meter 

0.25 0.25 -60 to 360/370 7EG-G011-11 

0.32 0.25 -60 to 360/370 7EM-G011-11 

20-Meter 

0.18 0.18 -60 to 360/370 7FD-G011-08 

25-Meter 

0.20 0.33 -60 to 360/370 7GE-G011-14 

30-Meter 

0.25 0.10 -60 to 360/370 7HG-G011-02 

0.25 0.25 -60 to 360/370 7HG-G011-11 

0.25 0.50 -60 to 360/370 7HG-G011-17 

0.25 1.00 -60 to 360/370 7HG-G011-22 

0.32 0.25 -60 to 360/370 7HM-G011-11 

0.32 1.00 -60 to 360/370 7HM-G011-22 

0.53 1.00 -60 to 360/370 7HK-G011-22 

60-Meter 

0.25 0.25 -60 to 360/370 7KG-G011-11 

0.25 1.00 -60 to 360/370 7KG-G011-22 

0.32 1.00 -60 to 360/370 7KM-G011-22 

Note: If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG-G011-11-B. Some 

exceptions may apply. Agilent 6850 and some SRI and process GC systems use only 5 in. cages. 

Contact Phenomenex or your local Phenomenex distributor 

for additional GC products and applications. 


Phenomenex

Zebron 



• First non-metal columns stable to 430 °C 

• Provides true boiling point separation for hydrocarbon 

distillation methods 

• Longer lifetime with rugged high temperature, polyimide 

coated, fused silica tubing 

• Low activity, provides good peak shape for acidic and 

basic samples 

• Provides robust column performance for high 

temperature bake outs 

• Individually tested for low bleed, MS certified 

• Temperature Limits: -60 to 400/430 °C 

(Isothermal/TPGC)* 

Alternative to Any 100 % Dimethylpolysiloxane High Temperature 

Phase: 

• DB-1ht 

• MXT-1 SimDist • Petrocol 2887 • CP-SimDist 


Low 

Polarity 

Bleed 


Stability 

Applications 

5 

• High Boiling Petroleum Products 

• Simulated Distillation Methods 

• Long-chained Hydrocarbons 

• High Molecular Weight Waxes 

• Polymers/Plastics 

• Motor Oils 


2007 R&D 100 

Award Recipient 

High 

Extended 

16148 

SimDist: ASTM Method D 6352 

0 

1 

2 

3 

Sample: 

1. C10 

2. C11 

3. C12 

4. C13 

5. C14 

6. C15 

7. C16 

8. C17 

9. Pristane 

10. C18 

4 

5 6 7 9 

31 33 

8 10 12 

11 13 14 15 

27 29 

1617 18 19 21 

23 

25 

20 22 

24 

26 

28 

11. Phytane 

12. C19 

13. C20 

14. C21 

15. C22 

16. C23 

17. C24 

18. C25 

19. C26 

20. C27 

21. C28 

22. C29 

23. C30 

24. C31 

25. C32 

26. C33 

27. C34 

28. C35 

29. C36 

30. C37 

30 

32 

34 

35 36 3738 

39 40 

41 

42434445 

46 

47 

48 

49 56 58 

5051 52 54 57 

53 55 

App ID 16148 

10 20 min 

Column: Zebron ZB-1HT Inferno 

Dimensions: 5 meter x 0.53 mm x 0.10 μm 

Part No.: 7AK-G014-02 

Injection: On-Column @ 43 °C, 0.1 μL 

Carrier Gas: Helium @ 4.4 mL/min (constant flow) 

Oven Program: 40 °C for 0.5 min to 430 °C @ 20 °C/min for 10 min 


31. C38 

32. C39 

33. C40 

34. C42 

35. C44 

36. C46 

37. C48 

38. C50 

39. C52 

40. C54 

41. C56 

42. C58 

43. C60 

44. C62 

45. C64 

46. C66 

47. C68 

48. C70 

49. C72 

50. C74 

Note: Sample was a combination of PolyWax 655 and retention time markers C8-C40 in 

CS2/Chloroform 

51. C76 

52. C78 

53. C80 

54. C82 

55. C84 

56. C86 

57. C88 

58. C90 


Zebron ZB-1HT Inferno GC Columns 


5-Meter 

0.53 0.10 -60 to 400/430 7AK-G014-02 

15-Meter 

0.25 0.10 -60 to 400/430 7EG-G014-02 

0.32 0.10 -60 to 400/430 7EM-G014-02 

0.32 0.25 -60 to 400/430 7EM-G014-11 

0.53 0.15 -60 to 400 7EK-G014-05 

20-Meter 

0.18 0.18 -60 to 400/430 7FD-G014-08 

30-Meter 

0.25 0.10 -60 to 400/430 7HG-G014-02 

0.25 0.25 -60 to 400/430 7HG-G014-11 

0.32 0.10 -60 to 400/430 7HM-G014-02 

0.32 0.25 -60 to 400/430 7HM-G014-11 

0.53 0.15 -60 to 400 7HK-G014-05 



*0.53 mm ID columns are rated to 400 ˚C max operational 

temperature. 

Contact Phenomenex or your local Phenomenex distributor for 

additional GC products and applications. 


13

Zebron 


ZB-5ms 

• A popular and rugged GC column for general use 

 

• Arylene Matrix Technology (AMT) provides a highly 

stable arylene phase 

• Fully conditioned within 35 minutes 

• High response for acids and bases = Very low activity 

• Enhanced resolution of Polyaromatic Hydrocarbons 

(PAHs) and other multi-ring aromatic compounds 


Low 

Polarity 

Bleed 


Stability 

8 

High 

• Our column of choice for EPA Methods 525, 610, 625, 

8100, and 8270 


• Temperature Limits: -60 to 325/350 ˚C (Isothermal/TPGC) 

Applications 

• Acids 

• Alkaloids 

• Amines 

• Dioxins 

• Drugs 

• Essential Oils/Flavors 

• FAMEs 

• Halo-hydrocarbons 

• PCBs/Aroclors 

• Phenols 

• Residual Solvents 

• Semi-volatiles 

• Solvent Impurities 

• Pesticides/Herbicides 

• EPA Methods 525, 610, 

625, 8100, 8270 

Alternative to Any MS-Certified 5 %-Phenyl-Arylene- 

95 %-Dimethylpolysiloxane Phase: 

• DB-5ms 

• DB-5ms EVDX 

Long Lifetime 

Consistent response after more than 700 samples at pH 2! 

Peak Skew 

1.5 

0.5 

1 

2.0 

2,4-Dichlorophenol 

Hexachlorobutadiene 

0 140 280 420 560 700 

Sample No. 

Reproducible Results 

2 

3 

4 5 

6 

• VF-5ms 

• CP-Sil 8 CB MS 

7 8 

3.0 4.0 5.0 6.0 7.0 8.0 min 




Injection: Split 1:100 @ 250 °C, 1.4 µL 

Carrier Gas: Hydrogen @ 140 °C, 40 cm/sec 

Oven Program: 140 °C (Isothermal) 


Sample: 1. Decane 

2. 2-Ethylhexanoic Acid 

3. 1,6-Hexanediol 

4. 4-Chlorophenol 

5. Tridecane 

6. 1-Methylnaphthalene 

7. 1-Undecanol 

8. Tetradecane 

9. Dicyclohexylamine 

10. Pentadecane 

9 

• DB-5.625 

• Rtx-5Sil MS 

10 

Lot 4 

Lot 3 

Lot 2 

Lot 1 


Zebron ZB-5ms GC Columns 


10-Meter 

0.10 0.10 -60 to 325/350 7CB-G010-02 

0.18 0.18 -60 to 325/350 7CD-G010-08 

12-Meter 

0.20 0.33 -60 to 325/350 7DE-G010-14 

15-Meter 

0.25 0.25 -60 to 325/350 7EG-G010-11 

20-Meter 

0.18 0.18 -60 to 325/350 7FD-G010-08 

0.18 0.32 -60 to 325/350 7FD-G010-51 

0.18 0.36 -60 to 325/350 7FD-G010-53 

25-Meter 

0.20 0.33 -60 to 325/350 7GE-G010-14 

30-Meter 

0.25 0.25 -60 to 325/350 7HG-G010-11 

0.25 0.50 -60 to 325/350 7HG-G010-17 

0.25 1.00 -60 to 325/350 7HG-G010-22 

0.32 0.25 -60 to 325/350 7HM-G010-11 

0.32 0.50 -60 to 325/350 7HM-G010-17 

0.32 1.00 -60 to 325/350 7HM-G010-22 

60-Meter 

0.25 0.25 -60 to 325/350 7KG-G010-11 

0.32 0.25 -60 to 325/350 7KM-G010-11 






Phenomenex

Zebron 



• First non-metal columns stable to 430 °C 

• Robust column for high temperature analysis, such as 

biodiesel, long-chain hydrocarbons, polymers, and high 

molecular weight compounds 

• Longer lifetime with rugged high temperature, polyimide 

coated, fused silica tubing 

• Low activity, provides good peak shape for acidic and 

basic samples 

• Individually tested for low bleed, MS certified 

• Temperature Limits: -60 to 400/430 °C (Isothermal/TPGC)* 


Low 

Polarity 

Bleed 


Stability 

Applications 

8 

• High Boiling Petroleum Products 

• Triglycerides 

• Simulated Distillation Methods 


• Long-chained Hydrocarbons 

• Motor Oils 

• Polymers/Plastics 

• Surfactants 

• High Molecular Weight Waxes 

High 

Alternative to Any 5 %-Phenyl-95 % Dimethylpolysiloxane High 

Temperature Phase: 

• DB-5ht • VF-5ht • HT-5 • Stx-5HT • XTI-5HT 

8.5 

Zebron Inferno Columns Win In The Lifetime Test 

pA 

Lower Bleed!* 

25 

23 

21 

19 

17 

15 

13 

11 

9 

Zebron ZB-5HT Inferno 

† 

Agilent ® J&W ® DB-5ht 

Retention of Pentadecane at 120 °C (min) 

8 

7.5 

7 

6.5 

Comparison of retention of Pentadecane between the Zebron ZB-5HT 

Inferno column versus a traditional 5 %-Phenyl - 95 %-dimethylpolysiloxane 

column. Note that the 5 %-Phenyl column died around 40 hours 

at 400 °C whereas the Zebron ZB-5HT Inferno column maintained great 

retention of Pentadecane over 100 hours. 

Zebron ZB-5HT Inferno 

† 

Agilent ® J&W ® DB-5ht 

† 

Varian ® VF-5ht 

7 

5 

0 5 10 15 20 25 30 35 min 

Conditions for all columns**: 


Injection: Null Injection @ 250 °C 

Carrier Gas: Hydrogen @ 11 psi (Constant Pressure) 

Oven Program: 120 °C for 3 min to 320 °C @ 30 °C/min (hold 5 min) to 

340 °C @ 30 °C/min (hold 5 min) to 360 °C @ 30 °C/min 

(hold 5 min) to 380 °C @ 30 °C/min (hold 5 min) to 400 °C 

@ 30 °C/min (hold 5 min) 


How does the lifetime test work? 

Hydrocarbons are a good way to measure the stability and 

lifetime of a non-polar column. Because its interaction with the 

phase is mostly based on London Dispersion forces, any change 

in retention time is correlated with phase loss. This will result in 

increased bleed and poor reproducibility. 

In the lifetime study above, the Zebron ZB-5HT Inferno column 

has twice the lifetime as the two comparative columns. For the 

test, all columns were held at 400 °C for 2 hours and then the 

oven was lowered to 120 °C for Pentadecane analysis. The Varian 

® HT column broke just after 40 hours at 400 °C. The Agilent ® 

J&W ® DB-5ht column had the same retention for Pentadecane at 

40 hours as the ZB-5HT at 100 hours. 

6 

0 20 40 60 80 100 120 

Hours at 400 ˚C 

Conditions for all columns**: 


Injection: 1.0 μL of test mix AG0-7578 


Oven Program: 120 °C Isothermal 

Detector: Flame Ionization Detector (FID) @ 400 °C 

Sample: Pentadecane 


Zebron ZB-5HT Inferno GC Columns 


15-Meter 

0.25 0.10 -60 to 400/430 7EG-G015-02 

0.25 0.25 -60 to 400/430 7EG-G015-11 

0.32 0.10 -60 to 400/430 7EM-G015-02 

0.32 0.25 -60 to 400/430 7EM-G015-11 

0.53 0.15 -60 to 400 7EK-G015-05 

15-Meter with 2-Meter Spliced Guard (0.53 mm ID) 

0.32 0.10 -60 to 400/430 7EM-G015-02-GST 

20-Meter 

0.18 0.18 -60 to 400/430 7FD-G015-08 

30-Meter 

0.25 0.10 -60 to 400/430 7HG-G015-02 

0.25 0.25 -60 to 400/430 7HG-G015-11 

0.32 0.10 -60 to 400/430 7HM-G015-02 

0.32 0.25 -60 to 400/430 7HM-G015-11 

0.53 0.15 -60 to 400 7HK-G015-05 



*The shaded area depicts the bleed criteria for MS certified columns on a MS detector. MS 

bleed certification values are typically read at 320 ºC. This demonstrates the low bleed 

capabilities of the ZB-5HT. It meets MS certification limits even at 360 ºC! 

**All columns used for above tests were new/never used, prior to this testing and purchased either 

directly from the original manufacturer or through an authorized distributor. All testing was carefully 

controlled to ensure conditions were similar for all columns involved. The comparative data may not 

be representative of every application. 

*0.53 mm ID columns are rated to 400 ˚C max operational 

temperature. 

† 

Agilent and J&W are registered trademarks of Agilent Technologies, Inc. Varian is a registered 

trademark under license to Varian, Inc. 


15

Zebron 


ZB-624 

• Widely used phase to separate volatile organic flavor 

and fragrance additives and residual solvents in industrial 

or pharmaceutical products 

• Popular choice for residual solvent testing 

• Excellent for US EPA Methods 501.3, 502.2, 503.1, 524.2, 

601, 602, 624, 8010, 8015, 8020, 8021, 8240, 8260 

• Specifically designed for the separation of volatile organic 

compounds (VOCs) 

• Increased temperature limit speeds run times and 

re-equilibration 


• Temperature Limits: -20 to 260 °C 


Low 

Polarity 

Bleed 


Stability 

Applications 

13 

• Volatile Organic Compounds (VOCs) 

• Residual Solvents 

• EPA Methods 501.3, 502.2, 503.1, 524.2, 601, 602, 624, 8010, 8015, 8020, 8021, 

8240, 8260 

• Pharmaceuticals 

High 

Alternative to Any 6 %-Cyanopropylphenyl- 

94 %-Dimethylpolysiloxane Phase: 

• DB-624 

• AT-624 

• DB-VRX 

• Rtx-1301 

• CP-1301 

• Rtx-624 

• BP624 

• 007-502 

• HP-VOC 

• 007-624 

• DB-1301 

• SPB-1301 

• CP-Select 624 CB 

• SPB-624 

• Rtx-VMS 

USP : Residual Solvents 

Procedure A - Class 1 

1 

4 6 8 10 12 min 

Column: Zebron ZB-624 


Part No.: 7HM-G005-31 

Injection: Split 5:1 @ 140 °C, 1 mL 

Carrier Gas: Helium @ 35 cm/sec (constant flow) 

Oven Program: 40 ˚C for 20 min to 240 ˚C @ 10 ˚C/min for 20 min 


Sample: 1. 1,1-Dichloroethene 

2. 1,1,1-Trichloroethane 

3. Carbon tetrachloride 

4. Benzene 

5. 1,2-Dichloroethane 

2 

3 

4 

5 

App ID 16348 


Zebron ZB-624 GC Columns 


20-Meter 

0.18 1.00 -20 to 260 7FD-G005-22 

30-Meter 

0.25 1.40 -20 to 260 7HG-G005-27 

0.32 1.80 -20 to 260 7HM-G005-31 

0.53 3.00 -20 to 260 7HK-G005-36 

60-Meter 

0.25 1.40 -20 to 260 7KG-G005-27 

0.32 1.80 -20 to 260 7KM-G005-31 

0.53 3.00 -20 to 260 7KK-G005-36 

75-Meter 

0.53 3.00 -20 to 260 7LK-G005-36 

105-Meter 

0.53 3.00 -20 to 260 7NK-G005-36 






Phenomenex

Zebron 


ZB-WAXp l u s 

• 100 % aqueous stable, excellent for aqueous samples 

• Extremely inert for acidic compounds 


Low 

High 

• Enhanced selectivity for low boiling solvents 

Polarity 

• High retention of alcohols and chlorinated solvents 

Bleed 

• Increased efficiency at 20 °C 

• Bonded and solvent rinsible 


Stability 


• Temperature Limits: 20 to 250/260 °C (Isothermal/TPGC)* 

Applications 

Alternative to Any Polyethylene Glycol Phase: 

• DB-WAX 

• Supelcowax-10 

• PEG 20M 

• AT-WAX 

• HP-20M 

• CP-WAX 52 CB 

• Stabilwax 

• AT-AquaWax 

• Carbowax 20M 

• Permabond CW 20M 

• CAM 

• BP20 

• Alcoholic Beverages 

• Alcohols 

• OVIs 

• Aldehydes 

• Aromatics 

• Acids (free) 

• Essential oils 

• Flavors / Fragrances 

• Styrene 

• Glycols 

• Pharmaceuticals 

• Solvents 

• Xylene Isomers 

Enhanced Aqueous Stability 

Wax columns provide optimal selectivity for many aqueous 

soluble compounds such as those found in alcoholic beverages 

or glycol samples. Historically, Polyethylene Glycol (PEG) phases 

have been unstable with aqueous samples resulting in poor reproducibility 

and decreased lifetime. The ZB-WAXp l u s bonding 

procedure results in exceptional stability to repeated injections 

of aqueous matrices. 

Water Reproducibility of ZB-WAXPLUS 

pA 

90 

80 

70 

60 

50 

40 

30 

20 

10 

0 

0 2 

1 

2 3 

4 

5 

4 6 8 10 12 14 

Column: Zebron ZB-WAXPLUS 





Oven Program: 35 ˚C for 5 min to 85 ˚C @ 10 ˚C/min to 200 ˚C @ 

25 ˚C/min for 1 min 


Sample: 1. Acetaldehyde 

2. Ethyl Acetate 

3. Methanol 

4. Ethanol 

5. Propanol 

6. Isobutanol 

7. 2-Methylbutanol 

8. 3-Methylbutanol 

6 

7,8 

Scottish Single 

Malt Whiskey 

60% 

Aqueous! 

min 

App ID 15817 


Zebron ZB-WAXPLUS GC Columns 


10-Meter 

0.10 0.10 20 to 250/260 7CB-G013-02 

15-Meter 

0.25 0.25 20 to 250/260 7EG-G013-11 

20-Meter 

0.18 0.18 20 to 250/260 7FD-G013-08 

30-Meter 

0.25 0.25 20 to 250/260 7HG-G013-11 

0.32 0.25 20 to 250/260 7HM-G013-11 

0.32 0.50 20 to 250/260 7HM-G013-17 

0.53 1.00 20 to 230/240 7HK-G013-22 

60-Meter 

0.25 0.25 20 to 250/260 7KG-G013-11 

0.32 0.50 20 to 250/260 7KM-G013-17 

0.53 1.00 20 to 230/240 7KK-G013-22 



*Thicker films (≥1.0 µm df) are rated to 230/240 ˚C 

(Isothermal/TPGC). 




17

Zebron 


~exclusive~ 

ZB-MultiResidue 

1 and 2 


• Proprietary phase specially designed for the separation of 

all types of pesticides, herbicides, and insecticides 

• Low activity, decreased breakdown of sensitive pesticides 

such as DDT 

• Provides robust column performance for high temperature 

bake outs 

• MS Certified phases provide low bleed performance for 

pesticide confirmation by MS 

• Temperature Limits: -60 to 320/340 °C 

(Isothermal/TPGC) 

Low 

Polarity MR-1 

Polarity MR-2 

Bleed 


Stability 

Applications 

• Organochlorine Pesticides 

• Insecticides 

• Organophosphorous Pesticides 

• Aroclors/PCBs 

11 

15 

• Nitrogen Containing Pesticides 

• Haloacetic Acids 

• Herbicides 

• Multi-pesticide Residue Methods 

High 

Similar* to: 

• Rtx-CLPesticides 

• Stx-CLPesticides 

• Rtx-CLPesticides2 

• Stx-CLPesticides2 

*not exact equivalent, selectivity might be different 

EPA Methods 508 and 8081A 

A 

1 

2 

3 

4 5 6 

8,7 

9 

10 

11 

17, 16 

13 

15 

14 

18 

12 

21,22 

20 

Baseline Resolution 

of EPA Method 8081 

19,23 

24 

25 

30,32 35 

39 

28 29 31 

36 

34 37 

33 38 

26 

27 

MR-2 

40 41 

App ID 16280 

B 

1 

2 

3 

4 5 6 

7 

8 

9 

14 

13 15 

17 

16 

10 

11 

12 

19,20 

18 

21,22 

23 24 25 

35 39 

2829 3031 36 

34 

33 3738 

32 

26 27 

MR-1 

4041 

App ID 16279 

2 4 6 8 10 12 14 16 min 

Column: A) Zebron MultiResidue-2 

B) Zebron MultiResidue-1 

Dimensions: A) 30 meter x 0.32 mm x 0.25 µm 

B) 30 meter x 0.32 mm x 0.50 µm 

Part No.: A) 7HM-G017-11 

B) 7HM-G016-17 

Injection: On-Column @ 123 °C, 1 µL 


Oven Program: 120 ˚C for 0.5 min to 210 ˚C @ 30 ˚C/min to 

300 ˚C @ 6 ˚C/min for 2 min 

Detector: ECD @ 340 °C 

Sample: All analytes are 50 ppb in isooctane 

1. DBCP 

2. Hexachlorocyclopentadiene 

3. Etridiazole 

4. Chloroneb 

5. Propachlor 

6. Diallate 

7. a-BHC 

8. Hexachlorobenzene 

9. g-BHC 

10. b-BHC 

11. Dichlone 

12. Alachlor 

13. d-BHC 

14. Chlorothalonil 

15. Heptachlor 

16. DCPA (Dacthal) 

17. Aldrin 

18. Heptachlor epoxide 

19. Captan 

20. trans-Chlordane (gamma) 

21. cis-Chlordane (alpha) 

22. trans-Nonachlor 

23. Endosulfan I 

24. DDE 

25. Dieldrin 

26. Chloropropylate 

27. Chlorobenzilate 

28. Endrin 

29. Nitrofen 

30. DDD 

31. Endosulfan II 

32. Kepone 

33. Trithion 

34. Endrin aldehyde 

35. DDT 

36. Endosulfan sulfate 

37. Captafol 

38. Methoxychlor 

39. Endrin ketone 

40. Permethrin 

41. Permethrin isomer 

2 4 6 8 10 12 14 16 18 min 

Ideal for Dual Column Analysis 

Injector 

5 m 

Z-Guard 

Zebron 

MR-1 

Zebron 

MR-2 

Y-Splitter 

ECD Detector 

ECD Detector 




Phenomenex

Zebron 


ZB-MultiResidue 

1 and 2 (cont’d) 


Zebron ZB-MultiResidue GC Columns (MR)-1 


30-Meter 

0.25 0.25 -60 to 320/340 7HG-G016-11 

0.32 0.50 -60 to 320/340 7HM-G016-17 

0.53 0.50 -60 to 320/340 7HK-G016-17 


Zebron ZB-MultiResidue GC Columns (MR)-2 


30-Meter 

0.25 0.20 -60 to 320/340 7HG-G017-10 

0.32 0.25 -60 to 320/340 7HM-G017-11 

0.53 0.50 -60 to 320/340 7HK-G017-17 

Note: If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG-G016-11-B or 

7HG-G017-11-B. Some exceptions may apply. Agilent 6850 and some SRI and process GC systems 

use only 5 in. cages. 

ZB-MultiResidue Column Kits 


0.25 mm ID 

(kit consists of products below) Part No.: KG0-8237 

Description Dimension Part No. 

MultiResidue MR-1 Column 30 meter x 0.25 mm x 0.25 µm df 7HG-G016-11 

MultiResidue MR-2 Column 30 meter x 0.25 mm x 0.20 µm df 7HG-G017-10 

Z-Guard 5 meter x 0.25 mm 7AG-G000-00-GZ0 

Universal Capillary Column Y-connector, Borosilicate 

AG0-4717 

0.32 mm ID 



MultiResidue MR-1 Column 30 meter x 0.32 mm x 0.50 µm df 7HM-G016-17 

MultiResidue MR-2 Column 30 meter x 0.32 mm x 0.25 µm df 7HM-G017-11 

Z-Guard 5 meter x 0.32 mm 7AM-G000-00-GZ0 


AG0-4717 

0.53 mm ID 



MultiResidue MR-1 Column 30 meter x 0.53 mm x 0.50 µm df 7HK-G016-17 

MultiResidue MR-2 Column 30 meter x 0.53 mm x 0.50 µm df 7HK-G017-17 

Z-Guard 5 meter x 0.53 mm 7AK-G000-00-GZ0 


AG0-4717 




19

Zebron 


~exclusive~ 

Zebron ZB-Drug-1 

• Optimized phase for the separation of drugs of abuse 

• Provides fast analysis with great peak shape 

• Improves resolution of target analytes from matrix 

interferences 

• Specially deactivated to improve quantitation for drug 

compounds 

• Temperature Limits: 40 to 320/340 °C 

Opiates on a ZB-5ms 

16733 

Applications 

• Drugs of Abuse 

Opiates on a ZB-Drug-1 

2009 R&D 100 


3,4 

17806 

1,2 

Unique 

Selectivity for Drugs 

3,4 

2 

1 

App ID 16733 

App ID 17806 

4.7 

4.8 4.9 5 5.1 5.2 5.3 5.4 5.5 5.6 5.7 5.8 5.9 6 min 

4.0 

4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 min 



Part No.: 7CD-G018-08 

Injection: Split 10:1 @ 240 °C, 1 µL 


Oven Program: 140 °C to 320 °C @ 20 °C for 1 min 

Detector: MSD @ 230 °C 

Sample: Analytes @ 10 pppm BSTFA/Pyridine 

1. Codeine-d6 

2. Codeine 

3. Morphine-d3 

4. Morphine 

Column: Zebron ZB-Drug-1 



Injection: Split 10:1 @ 280 °C, 1 µL 


Oven Program: 180 °C to 340 °C @ 20 °C/min 


Sample: Analytes @ 10 pppm BSTFA/Pyridine 

1. Codeine-d6 

2. Codeine 

3. Morphine-d3 

4. Morphine 

17809 

Amphetamines 

2 

1 

3 

4 

5 

6 

8 

7 

9 

10 

App ID 17809 


Zebron ZB-Drug-1 GC Columns 


10-Meter 

0.18 0.18 40 to 320/340 7CD-G023-08 

15-Meter 

0.25 0.25 40 to 320/340 7EG-G023-11 

30-Meter 

0.25 0.25 40 to 320/340 7HG-G023-11 



3 

4 5 6 min 

Column: Zebron ZB-Drug-1 





Oven Program: 70 °C to 130 °C @ 20 °C/min to 270 ºC @ 30 ºC 


Sample: Analytes are 40 ppm derivatized with HFBA 

1. Amphetamine-d11 

2. Amphetamine 

3. Methamphetamine-d14 

4. Methamphetamine 

5. MDA-d5 

6. MDA 

7. MDMA-d5 

8. MDMA 

9. MDEA-d5 

10. MDEA 




Phenomenex

Zebron 


n e 

w 

ZB-1XT SimDist Metal 

 

• New Glass Infusion technology provides higher 

efficiency and greater column-to-column reproducibility 

• Guaranteed high quality - every column is individually 

tested 

• Up to 70% more efficiency than other columns 

• Improved resolution of C50/C52 

• Increased accuracy for high temperature simulated 

distillation analysis 

• Temperature Limits: -60 to 450 °C 

(Isothermal/TPGC)* 

Alternative to: 

 

• Varian CP-SimDist UltiMetal 

® 

• Restek MXT ® -1HT SimDist 

19264 

® 

• Agilent J&W DB-HT SimDis 


Low 

Polarity 

Bleed 


Stability 

Applications 

5 

• Crude Oil 

• Vacuum Distillates 

• Petroleum Distillates 

• Petroleum Fractions 

High 

• Gasoline Fractions 

• Simulated Distillations 

• ASTM Methods D2887, D2887X, D3710, 

D6352, D7169 

Hydrocarbons C7–C100+: ASTM Method D7169 

C8 

C7 

High Efficiency! 

C9 

C44 

C50 

Resolution: 4.81 

C52 

0 

10 20 30 40 min 

Column: Zebron ZB-1XT SimDist 


Part No.: 7AK-G026-05 

Injection: On-Column @ 33 °C, 1 µL 

Carrier Gas: Helium @ 7 mL/min (constant flow) 

Oven Program: 30 °C to 450 °C @ 10 °C/min for 10 min 


Sample: C7 to C44 hydrocarbons and POLYWAX ® 655 

Note: Sample in CS2. Chromatogram is baseline subtracted. 

C60 

C70 

C80 

C90 

C100 

App ID 19264 


Zebron ZB-1XT SimDist Metal 


5-Meter 

0.53 0.09 -60 to 450 7AK-G026-55 

0.53 0.15 -60 to 450 7AK-G026-05 

10-Meter 

0.53 0.15 -60 to 450 7CK-G026-05 

0.53 0.88 -60 to 400 7CK-G026-49 

0.53 2.65 -60 to 400 7CK-G026-35 

If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7CK-G026-05-B. Some 


*Thicker film (2.65 µm df) is rated to 400 ˚C 

(Isothermal/TPGC). 




21



HPLC Column 

Protection Kit 

Chiral HPLC Analysis 

Lux ® 

Kinetex 

Ultra High Performance 

on ANY LC system 

GPC 

Phenogel 

Protein/Peptide Analysis & 

Purification 

Jupiter ® 300 RP-HPLC 

Jupiter Proteo RP-HPLC 

BioSep GFC/SEC 

Carbohydrate/Organic 

Acid Analysis 

Rezex 

HPLC 

Column Selection 

p. 23 

Lux 

HPLC Chiral Analysis 

p. 42-44 

SecurityGuard 

HPLC Column Protection 

p. 24-25 

BioSep 

Size Exclusion (GFC) for 

Proteins/Peptides 

p. 45-47 

Kinetex 

Ultra High Performance 

Gemini ® 

pH Stable 1-12 

Synergi 

Full Range Selectivity 

p. 26-35 

p. 36-37 

p. 38-39 

Jupiter 

RP-HPLC for 

Protein/Peptide Analysis 

Rezex 

Carbohydrate and 

Organic Acid Analysis 

p. 48-51 

p. 52-54 

Luna ® 

One of the World’s 

Leading HPLC Columns 

p. 40-41 

Phenogel 

Size Exclusion (GPC) 

AXIA 


p. 55-56 

p. 57-60 


Phenomenex

HPLC Column Selection 

Sample 

MW 

Sample 

Solubility 

Separation 

Mode 

Our Recommended 

Column 

Page 

Hexane- 

Soluble 

Normal Phase 

Adsorption 

Normal Phase 

Bonded 

Kinetex HILIC 26 

Luna Silica(2) 40 

Onyx Silica * 

Luna CN, NH 2 

, HILIC 40 

Organic- 

Soluble 

Methanol 

Methanol/H 2 

0 

Soluble 

Reversed Phase 

Bonded 

Chiral 

Kinetex C18, XB-C18, C8 26 

Synergi Max-RP, Fusion-RP 38 

Luna C8(2), C18(2) 40 

Gemini C18, Gemini-NX C18 36 

Lux 42 

THF-Soluble 

Gel Permeation 

GPC 

Phenogel 50 Å, 100 Å 55 

Shodex GPC * 

MW5000 

Gel Filtration 

Aqueous 

GFC/SEC 

pH 2-7.5 

pH > 7.5 

BioSep-SEC-S Series 45 

Shodex OHpak * 

PolySep-GFC-P * 

Aqueous- 

Soluble 

Ion-Exchange 

Cation- 

Exchange 

Anion- 

Exchange 

pH 2-10 

Shodex IEC CM-825 * 

Shodex IEC DEAE * 

Clarity Oligo-WAX * 

Jupiter 300 C4, C5, C18 48 

Reversed Phase 

pH > 10 

PolymerX RP-1 * 

Hamilton PRP-3 * 

Hydrophobic 

Interaction (HIC) 

Shodex HIC * 

Affinity/Bioaffinity 

Shodex AFpak * 

*Visit www.phenomenex.com for products not featured in this guide. 


23



• Protect HPLC columns and extend lifetime 

• Virtually no change in chromatography 

• Available in analytical, semi-prep, and preparative sizes 

• Simple to use 

Did you know a common cause of high backpressure, split 

peaks, broad peaks, baseline noise, baseline drift and loss of 

resolution is contaminants? The fact is all mobile phases contain 

some chemical contaminants or microparticulates, from the 

sample, solvent, or wear on the polymeric seals of the pump or 

injector. These contaminants can clog frits, irreversibly bind to an 

HPLC column, degrade performance, and even damage the flow 

cell. An easy solution, SecurityGuard is a universal HPLC guard 

cartridge system designed to effectively (and inexpensively), 

protect your valuable columns, from the damaging effects of 

chemical contaminants, without altering your chromatographic 

results. 

A Universal Guard Cartridge System 

How It Works* 

The SecurityGuard analytical cartridge holder (patented) 

directly finger-tightens into virtually any manufacturer’s column. 

Contaminants are retained by an inexpensive, 4 mm, disposable 

cartridge instead of fouling your expensive analytical column. 

Cutaway view showing 

Cartridge - can be easily 

inspected for contaminants 

Fingertight connection to 

virtually any female inverted 

endfitting worldwide 

Universal Fingertight connection to HPLC 

column - no wrenches required 

* 

contaminants 

compounds of interest 

From injector 

To detector 

Compare, Virtually No Change 

mAU 

10 

HPLC column 

0 

-10 

-20 

-30 

-40 

mAU 

10 

0 

-10 

-20 

-30 

-40 

0 5 10 15 20 

Without SecurityGuard 

min 

vs. 

App ID 10731 

With SecurityGuard 

App ID 10731 

Columns: As specified within App ID 

Dimensions: 150 x 4.6 mm 

Guard Cartridge: SecurityGuard C18 (ODS) 4 x 3.0 mm ID 

Mobile Phase: 0.1 M Ammonium acetate/Acetonitrile/Methanol (35:25:40) 

Flow Rate: 0.6 mL/min 

Detection: UV @ 245 nm 

Sample: 1. Clonazepam 

2. Oxazepam 

3. Diazepam 

4. Bromazepam 

5. Temazepam 

6. Medazepam 

* Feature applies to analytical-sized guard system only, and does not 

apply to SemiPrep or PREP guard cartridges. 

0 5 10 15 20 

min 

SecurityGuard is a trademark of Phenomenex. 


Phenomenex


SecurityGuard 

Cartridges and Holders 

Step 1: Choose column ID 

Step 2: Match column phase 

Choose your HPLC Column Internal Diameter, ID (mm): 

2.0 - 3.0 3.2 - 8.0 9.0 - 16.0 18.0 - 29.0 30.0 - 49.0 

Analytical SemiPrep PREP 

Use Cartridges (mm): 

4.0 x 2.0 4.0 x 3.0 10 x 10.0 15 x 21.2 15 x 30.0 


Material Description pH Stability 

Cartridges for General Purpose/Pharmaceutical /10pk /10pk /3pk ea ea 

C18 (ODS, Octadecyl) 1.5 - 10 AJ0-4286 AJ0-4287 AJ0-7221 AJ0-7839 AJ0-8301 

C12 (Dodecyl) 1.5 - 10 AJ0-6073 AJ0-6074 AJ0-7275 AJ0-7842 AJ0-8304 

C8 (MOS, Octyl) 1.5 - 10 AJ0-4289 AJ0-4290 AJ0-7222 AJ0-7840 AJ0-8302 

C5 (Pentyl) 1.5 - 10 AJ0-4292 AJ0-4293 AJ0-7372 — — 

C1 (TMS) 2 - 9 AJ0-4298 AJ0-4299 AJ0-7373 — — 

Silica — — AJ0-4347 AJ0-4348 AJ0-7223 AJ0-7229 AJ0-8312 

HILIC HILIC 1.5 - 8 AJ0-8328 AJ0-8329 — — — 

NH 2 

(Amino, Aminopropyl) 1.5 - 11 AJ0-4301 AJ0-4302 AJ0-7364 AJ0-8162 AJ0-8309 

CN (Cyano, Cyanopropyl) 2 - 7.5 AJ0-4304 AJ0-4305 AJ0-7313 AJ0-8220 AJ0-8311 

Phenyl (Phenylhexyl) 1.5 - 10 AJ0-4350 AJ0-4351 AJ0-7314 AJ0-7841 AJ0-8303 

PFP(2) Pentafluorophenyl 1.5 - 8 AJ0-8326 AJ0-8327 AJ0-8376 AJ0-8377 AJ0-8378 

SCX (SA, Strong Cation Exchanger) 2.5 - 7.5 AJ0-4307 AJ0-4308 AJ0-7369 AJ0-8595 AJ0-8596 

SAX (SB, Strong Anion Exchanger) 2.5 - 7.5 AJ0-4310 AJ0-4311 AJ0-7370 — — 

RP-1 (Reversed Phase - Polymer) 0 - 14 AJ0-5808 AJ0-5809 AJ0-7368 AJ0-8358 — 

Polar-RP (Ether-linked Phenyl) 1.5 - 7 AJ0-6075 AJ0-6076 AJ0-7276 AJ0-7845 AJ0-8307 

Fusion-RP (C18 Polar Embedded) 1.5 - 10 AJ0-7556 AJ0-7557 AJ0-7558 AJ0-7844 AJ0-8306 

AQ C18 (Polar Endcapped C18) 1.5 - 7.5 AJ0-7510 AJ0-7511 AJ0-7512 AJ0-7843 AJ0-8305 

Gemini-NX (C18 Twin-NX Technology) 1 - 12 AJ0-8367 AJ0-8368 AJ0-8369 AJ0-8370 AJ0-8371 

Gemini C18 (C18 Twin Technology) 1 - 12 AJ0-7596 AJ0-7597 AJ0-7598 AJ0-7846 AJ0-8308 

Gemini C6-Phenyl (C6-Phenyl Twin Technology) 1 - 12 AJ0-7914 AJ0-7915 — — — 

Oligo-RP (C18 Twin Technology) 1 - 12 AJ0-8134 AJ0-8135 AJ0-8136 AJ0-8210 AJ0-8310 

Oligo-WAX (WA, Weak Anion Exchanger) 1.5 - 11 — AJ0-8324 AJ0-8325 AJ0-8639 AJ0-8420 

Cartridges for Protein and Polypeptide Reversed Phase /10pk /10pk /3pk ea ea 

For use with silica columns for separation of proteins & peptides, such as Jupiter ® (Phenomenex); Vydac ® 218TP, 214TP (Alltech Associates, Inc.); Nucleosil ® 300 Å C18, C4; 

Hypersil ® 300 Å, and other widepore or 300 Å brands. 

Widepore C18 (ODS, Octadecyl) 1.5 - 10 AJ0-4320 AJ0-4321 AJ0-7224 AJ0-7230 AJ0-8313 

Widepore C5 (Pentyl) 1.5 - 10 AJ0-4326 AJ0-4327 AJ0-7371 — — 

Widepore C4 (Butyl) 1.5 - 10 AJ0-4329 AJ0-4330 AJ0-7225 AJ0-7231 AJ0-8314 

Cartridges for Silica GFC — /10pk /3pk ea — 

(Aqueous SEC) For use with silica GFC columns, such as BioSep (Phenomenex); ZORBAX ® GF-Series; Bio-Sil ® (Bio-Rad). 

GFC-2000 — 2 - 7.5 — AJ0-4487 AJ0-7365 AJ0-8588 — 

GFC-3000 — 2 - 7.5 — AJ0-4488 AJ0-7366 AJ0-8589 — 

GFC-4000 — 2 - 7.5 — AJ0-4489 AJ0-7367 AJ0-8590 — 

Cartridges for Chiral /10pk /10pk /3pk ea ea 

For use with chiral columns, such as Lux ® Cellulose-1, -2, -3, -4, & Amylose-2 (Phenomenex); CHIRALCEL ® OD-H ® , CHIRALCEL ® OJ-H ® & Chiralpak ® Ad ® -H (DAICEL Chemical 

Industries, Ltd.) 

Lux Cellulose-1 Cellulose tris (3, 5-dimethylphenylcarbamate) 

2 - 9 AJ0-8402 AJ0-8403 AJ0-8404 AJ0-8405 AJ0-8406 

Lux Cellulose-2 Cellulose tris (3-chloro-4- 2 - 9 AJ0-8398 AJ0-8366 AJ0-8399 AJ0-8400 AJ0-8401 

methylphenylcarbamate) 

Lux Cellulose-3 Cellulose tris (4-methylbenzoate) 2 - 9 AJ0-8621 AJ0-8622 AJ0-8623 AJ0-8624 AJ0-8625 

Lux Cellulose-4 Cellulose tris (4-chloro-3-methylphenylcarbamate) 

2 - 9 AJ0-8626 AJ0-8627 AJ0-8628 AJ0-8629 AJ0-8630 

Lux Amylose-2 Amylose tris (5-chloro-2-methylphenylcarbamate) 

2 - 9 AJ0-8471 AJ0-8470 AJ0-8472 AJ0-8473 AJ0-8474 

Cartridges for Carbohydrate/Organic Acid — /10pk — — — 

For organic acid and carbohydrate analysis, such as Rezex (Phenomenex); Aminex ® (Bio-Rad); Interaction; Sugar-Pak (Waters). 

Carbo-H + — 1 - 8 — AJ0-4490 — — — 

Carbo-Ag +* — Neutral — AJ0-4491 — — — 

Carbo-Pb +2 — Neutral — AJ0-4492 — — — 

Carbo-Ca +2 — Neutral — AJ0-4493 — — — 

Guard Cartridge Holders (one-time purchase only) /kit /holder /kit /kit 

KJ0-4282 AJ0-7220 AJ0-8223 AJ0-8277 

*For use with saccharide and oligosaccharide columns in Ag + form. 


25

Kinetex 

HPLC/UHPLC 

Ultra-High Performance 

n e 

w 

new 

new 

new 

new 

Ultra-High Performance on Any LC System 

• Achieve sub-2 µm performance within HPLC backpressure limitations 

• Substitute 3 µm and 5 µm columns for 2-3x higher efficiency 

• Obtain higher throughput without sacrificing resolution 

• Easy method transfer across LC system platforms 

• Reduce solvent consumption with faster analysis 

• Reach lower levels of detection and quantitation 

Material Characteristics* 

Total 

Particle 

Size 

(µm) 

Porous 

Shell 

(µm) 

Solid 

Core 

(µm) 

Packing 

pH 

Material 

Stability 

Kinetex XB-C18 2.6 0.35 1.9 100 200 10 1.5 - 10** 

Pore 

Size 

(Å) 

Effective 

Surface 

Area 

(m 2 /g) 

Effective 

Carbon 

Load 

% 

Kinetex C18 2.6 0.35 1.9 100 200 12 1.5 - 10** 

Kinetex C8 2.6 0.35 1.9 100 200 8 1.5 - 8.0 

Kinetex PFP 2.6 0.35 1.9 100 200 9 1.5 - 8.0 

Kinetex HILIC 2.6 0.35 1.9 100 200 0 2.0 - 7.5 

Kinetex XB-C18 1.7 0.23 1.25 100 200 10 1.5 - 10** 

Kinetex C18 1.7 0.23 1.25 100 200 12 1.5 - 10** 

Kinetex C8 1.7 0.23 1.25 100 200 8 1.5 - 8.0 

Kinetex PFP 1.7 0.23 1.25 100 200 9 1.5 - 8.0 

Kinetex HILIC 1.7 0.23 1.25 100 200 0 2.0 - 7.5 

*For the evaluation of effective surface area and carbon load, please request technical note, TN-1064 

**Columns are pH stable from 1.5-10 under isocratic conditions. Columns are pH stable 2-8 under gradient conditions. 

2.1 mm ID Kinetex columns are pressure stable up to 1000 bar. 

Pressure 

Stability 

1000/600 

bar 

1000 bar 

2010 R&D 100 


Innovation in Particle Technology 

The Kinetex core-shell particle is not fully porous. Using sol-gel processing techniques that incorporate 

nano structuring technology, a durable, homogenous porous shell is grown on a solid silica core. 

This highly optimized process combined with uniform particle size distribution produces a column 

that generates extremely high plate counts. When using Kinetex 2.6 µm, less column backpressure is 

generated, allowing it to be used on any LC system. † 

Kinetex 1.7 µm 

Core-Shell Particle 

• Reduced diffusion path 

maximizes efficiency 

• Increased efficiencies 

compared to traditional 

fully porous sub-2 µm 

columns. Typical operating 

backpressures > 400 bar 


Core-Shell Particle 

• Reduced diffusion path 

maximizes efficiency 

• Ultra-high performance 

on any system with 

Kinetex 2.6 µm columns 

1.9 µm Solid Core 

0.35 µm Porous Shell 

1.25 µm Solid Core 

0.23 µm Porous Shell 

1.7 µm 

2.6 µm 

 

 

SEM of Kinetex Core-Shell Particles 

Cross Section of Kinetex Core-Shell Particle 

† 

When using Kinetex 1.7 µm, increased performance can be achieved, however higher pressure-capable instrumentation is required. 


Phenomenex

Kinetex 

HPLC/UHPLC 


Complementary and Orthogonal Selectivities 

To provide alternative and orthogonal selectivity phases, Kinetex columns are available in 5 selectivities: XB-C18, 

C18, C8, PFP (Pentafluorophenyl), and HILIC (Hydrophilic Interaction Liquid Chromatography), for resolution of a 

wide range of compounds from polar to hydrophobic, aromatic, and isomers. 

NEW! Kinetex XB-C18 

Protective butyl side-chains allow 

superior peak shape and enhanced 

separation of basic compounds 

Kinetex C18 

Offers the hydrophobic retention 

and methylene selectivity 

chromatographers expect from a 

C18 column. 

Low 

High 

TMS 

TMS 

Low 

High 

TMS 

Si 

TMS 

Hydrophobicity: 


Polarity: 

Polarity: 

XB- 

C18 

 

H-Bonding: 

Aromatic Selectivity: 

Silanol Activity: 

pH Range: 1.5 – 10* 

USP Classification: L1 

Particle Sizes: 2.6 µm & 1.7 µm 

Effective Carbon Load: 10 % 

C18 

 

H-Bonding: 



pH Range: 1.5 – 10* 




NEW! Kinetex C8 

TMS 

C8 

TMS 

 

Brings the benefits of core-shell 

technology to USP L7 and other C8 

column methods. 


Polarity: 

H-Bonding: 



Low 

pH Range: 1.5 – 10* 




High 

Kinetex PFP 

F 

F 

F 

F 

F 

TMS TMS 

PFP 

 

Pentafluorophenyl phase 

incorporates fluorine atoms on 

the periphery of the phenyl ring to 

provide unique aromatic and polar 

selectivity. 


Polarity: 

H-Bonding: 



Low 

pH Range: 1.5 – 8 




High 

Kinetex HILIC 

H 20 

ACN ACN ACN ACN 



H 20 


Unbonded silica phase used under 

hydrophilic interaction chromatography 

conditions to provide 

selectivity for the retention and 

separation of polar compounds. 

H 20 

HO 

HO 

O 

H 20 

OH 

H 20 

H 20 

O 

H 20 

HO 

H 20 Si 

H 20 

OH 

Si 

HILIC 

Si 

 

OH 


Polarity: 

H-Bonding: 



Low 

pH Range: 2.0 – 7.5 



Carbon Load: – 

High 

*Columns are pH stable from 1.5-10 under isocratic conditions. Columns are pH stable 2-8 under gradient conditions. 


27

Kinetex 

HPLC/UHPLC 


Optimized for Ultra-High 

Performance 

Faster Mass Transfer 

Since the Kinetex particle is not fully porous, analytes 

spend less time diffusing into and out of the pores as 

they travel through the column. This shorter diffusion path 

allows for faster mass transfer. The result is less band 

broadening for higher peak efficiency comparable to or better 

than sub-2 µm fully porous particles. 

High Efficiency over Extended Range of Flow Rates 

30 

25 

2.6 µm Kinetex Core-Shell 

 

Sub-2 µm Fully Porous 

Band Broadening 


HETP (µm) 

20 

15 

10 

Double the efficiency 

of 3 µm at high speed 

Sub-2 µm exceeds 

conventional pressure here 

3 µm 

3 µm Fully Porous 

5 

0 

0 

1 2 3 4 5 

Linear Velocity (mm/s) 

6 7 8 9 10 

Kinetex 2.6 µm Core-Shell 

Sub-2 µm Fully Porous 

3 µm Fully Porous 


18755 

18756 

Kinetex particles are nearly monodispersed. This extremely narrow particle size distribution results in ultra-high 

column efficiency and excellent reproducibility. 

Uniform Particle Size Distribution 

Particle Count 

3000 

2000 

1000 

0 

1 2 3 

Particle Diameter (µm) 

Kinetex 2.6 µm Core-Shell 

Traditional Fully Porous 1.7 µm 

Traditional Fully Porous 3 µm 

4 5 6 

Batch-to-Batch Reproducibility Overlay 

0 

1 

2 

3 

1 

Conditions same for all batches: 

Column: Kinetex 2.6 µm C18 


Part No.: 00B-4462-E0 

Mobile Phase: Water / Acetonitrile (65:35) 



Sample: 1. Uracil 

2. Hydroxycortisone 

3. Cortisone 

4. Cortisone acetate 

5. 17-Hydroxyprogesterone 

4 

Batch 68 R s 

(2,3) 2.00 

Batch 73 R s 

(2,3) 2.13 

Batch 75 R s 

(2,3) 2.06 

2 3 4 5 min 

5 

Batch 68 

Batch 73 

Batch 75 

App ID 18755, 18756, 18757 


Phenomenex

Kinetex 

HPLC/UHPLC 


Achieve Sub-2 µm Performance within HPLC Backpressure Limitations 

With the efficiency of a sub-2 µm column and typical operating backpressure less than 

400 bar † , you can achieve the promise of ultra-high performance on any LC system. 

18165 

mV 

400 

1 

Traditional 1.7 µm 

N = 272,080 p/m 

> 400 bar! 

Method cannot transfer to 

a non-UHPLC instrument 

18166 

mV 

400 

1 


N = 267,720 p/m 

< 300 bar! 

Easier method transfer 

to ANY system 

200 

2 

3 

4 

200 

2 

3 

4 

0 

0 

0 1 2 min 

0 

1 2 min 

Unparalleled Levels of Ultra-High Performance 

For users of higher pressure capable instruments who want the highest level of efficiency, 

we introduce the Kinetex 1.7 µm column - the first sub-2 µm core-shell particle available 

on the market. 

18165 

mV 

400 

1 

Traditional 1.7 µm 

N = 272,080 p/m 

18167 

mV 

400 


N = 318,680 p/m 

1 

200 

2 

3 

4 

200 

2 

3 

4 

App ID 18165 

App ID 18167 

App ID 18165 

App ID 18166 

Conditions for both columns: 


Traditional 1.7 µm C18 


Mobile Phase: Acetonitrile / Water (50:50) 


Temperature: 25 °C 


Instrument: *Waters ® ACQUITY ® UPLC ® 

Sample: 0.5 µL test mixture 

1. Acetophenone 

2. Benzene 

3. Toluene 


0 

0 

0 1 2 min 

0 1 2 min 



Traditional 1.7 µm C18 






Instrument: *Waters ® ACQUITY ® UPLC ® 

Sample: 


2. Benzene 

3. Toluene 


† 

Kinetex 2.6 µm columns, 2.1 mm ID, are pressure rated to 1000 bar use on both HPLC and UHPLC instrumentation. 

* Waters, ACQUITY, and UPLC are registered trademarks of Waters Corporation. Phenomenex is not affiliated with Waters Corporation. 

Comparative separations may not be representative of all applications. 


29

Kinetex 

HPLC/UHPLC 


Substitute 3 µm and 5 µm Columns for 2-3x Higher Efficiency 

18628 

Replace traditional 3 µm or 5 µm analytical columns with Kinetex 2.6 µm core-shell columns for immediate performance improvements 

in efficiency, speed, resolution, and sensitivity. Optimize methods for ultra-high performance and transfer them to any system. 

mV 

240 

210 

180 

150 

120 

90 

60 

30 

0 

18280 

0 2 

1 

1 

2 

2 

3 

4 

3 

5 

4 

6 

Traditional 3 µm 

N = 166,502 p/m 

7 



Traditional 3 µm C18 





Backpressure: 380 bar (Kinetex) 

250 bar (Traditional 3 µm) 


Instrument: Agilent 1200SL 

8 

Sample: 

9 

4 

10 

11 

App ID 18628 

min 

1. Uracil 


12 13 

18539 

mV 

240 

210 

180 

150 

120 

90 

60 

30 

0 

1 

2 

0 2 

3. Toluene 


Obtain Higher Throughput without Sacrificing Resolution 

3 

4 


N = 295,343 p/m 

4 min 

For the ultimate sample throughput demands, Kinetex columns provide 18276 the efficiency needed to significantly reduce run times. In this 

separation of 13 ketones, a 20-fold increase in productivity is accomplished while still maintaining resolution. 

Traditional 5 µm 

> 60 min 

Rs 10,11 

= 3.11 

App ID 18280 

1 

2 

3 

4 

5 

6 

7 

8 

< 3 min 

Resolution maintained 

Rs 10,11 

= 3.12 

9 

10 

11 

12 

13 

App ID 18539 


App ID 18276 

0 

Column: Traditional 5 µm C18 


Mobile Phase: A: Water 

B: Acetonitrile 

Gradient: Time (min) % B 

0 5 

4.78 5 

51.52 95 




Sample: 

20 40 60 min 

1. Acetone 

2. 2-Butanone 

3. 2-Pentanone 


5. 2-Heptanone 

6. Butyrophenone 

7. 2-Nonanone 

Time (min) % B 

66 95 

66.01 5 

86.38 5 

8. Hexanophenone 

9. Octanophenone 

10. 2-Tridecanone 

11. Decanophenone 

12. 2-Pentadecanone 

13. 2-Hexadecanone 


0 1 

2 min 



Part No.: 00B-4462-E0 




0 5 

0.23 5 

2.19 95 




Backpressure: 350 bar 

Sample: 

1. Acetone 

2. 2-Butanone 

3. 2-Pentanone 


5. 2-Heptanone 

6. Butyrophenone 

7. 2-Nonanone 


2.75 95 

2.76 5 

3.61 5 


9. Octanophenone 

10. 2-Tridecanone 

11. Decanophenone 

12. 2-Pentadecanone 

13. 2-Hexadecanone 


Phenomenex

Kinetex 

HPLC/UHPLC 


Easy Method Transfer Across LC System Platforms 

UHPLC methods developed with fully porous sub-2 µm columns often generate 

backpressure higher than HPLC system limitations. With Kinetex 2.6 µm 

core-shell technology, you are no longer restricted from developing high performance 

LC methods on any system and transferring them anywhere. In these 

examples different internal diameters of Kinetex columns are used on various 

systems to illustrate the versatility of Kinetex core-shell technology. Please note 

the flow rates are scaled to maintain the same linear velocity. 

Kinetex 4.6 mm ID 

on Agilent 1100 



Part No.: 00B-4462-E0 



Temperature: Ambient 




3. Toluene 



on *Shimadzu Prominence UFLCx r 



Part No.: 00B-4462-Y0 







18567 

3. Toluene 


4 

2 3 

4 

3 

1 

2 

App ID 18565 

1 

App ID 18567 

0 

1 2 min 

0 1 2 min 


on Agilent 1200SL 



Part No.: 00B-4462-AN 







3. Toluene 



on *Waters ® ACQUITY ® UPLC ® 








18568 Sample: 1. Uracil 


3. Toluene 


4 

2 

3 

2 3 

4 

1 

App ID 18566 

1 

App ID 18568 

0 1 

2 min 

0 

1 

2 min 

* Waters, ACQUITY, and UPLC are registered trademarks of Waters Corporation. Prominence and UFLC are trademarks of 

Shimadzu Corporation. Phenomenex is not affiliated with Agilent Technologies, Waters Corp. or Shimadzu. 


31

Kinetex 

HPLC/UHPLC 


Improve Performance, Save Solvent 

When chromatographic column performance improves you can not only decrease your analysis time but also 

decrease your overall solvent consumption without compromising your separations. Use Kinetex core-shell technology 

to dramatically decrease the solvent consumption in your laboratory and increase sample throughput. 

Column: Traditional 5 µm C18 



18243 

Example Method Consumption 

2 

4 

5 

6 

110 mL 

solvent per run! 

1 

3 

7 8 

9 

10 

App ID 18243 

0 

20 40 60 80 100 120 min 





Less Solvent Consumption with Kinetex Column 

18245 

2 

4 

5 

6 

< 4 mL 

solvent per run! 

1 

3 

7 8 

9 

10 

App ID 18245 

0 

1 2 3 4 min 


Mobile Phase: A: 20 mM Potassium phosphate pH 7 

B: Methanol / Acetonitrile (50:50) 

A/B (48:52) 

Temperature: 40 ºC 


Sample: 

1. Tianeptine 

2. Desmethyldoxepin 

3. Protriptyline 

4. Desipramine 

5. Imipramine 

6. Amoxapine 

7. Doxepin 

8. Nortriptyline 

9. Amitriptyline 

10. Clomipramine 

Reach Lower Levels of Detection and Quantitation 

The combination of the small particle size, narrow particle size distribution, and the significantly shorter diffusion path results in much higher 

column efficiencies and increased chromatographic resolution. The increased efficiencies provide an immediate benefit on sensitivity since 

higher chromatographic efficiencies translate into significantly narrower and taller peaks, making it easier to detect low level impurities. 

18262 

*ZORBAX ® 3.5 µm SB-C18 

18263 

Kinetex 2.6 µm C18 

mAU 

mAU 

0.8 

0.8 

0.6 

0.6 

0.4 

0.4 

0.2 

0.0 

App ID 18263 

0.2 

0.0 

App ID 18262 

4 6 min 

4 6 min 





Gradient: (95:5) A/B for 1.16 min, then to (5:95) A/B 




Instrument: Agilent 1200 

Sample: 

1. Pyridine 

2. Acetaminophen 

3. Pindolol 

4. Quinine 

5. Acebutolol 

6. Chlorpheniramine 

7. Triprolidine 

8. Prednisolone 

9. Nortriptyline 

10. 4-Chlorobenzoic acid 

11. 5-Methyl-2-hydroxy benzaldehyde 

12. 4-Chlorocinnamic acid 

13. Diazepam 

14. Diflunisal 

15. Niflumic acid 


* Zorbax is a registered trademark of Agilent Technologies. Comparative separations may not be representative of all applications. 

Phenomenex is not affiliated with Agilent Technologies. 


Phenomenex

Kinetex 

HPLC/UHPLC 


For more applications on our Kinetex HPLC columns, contact your local Phenomenex Distributor. 

Veterinary Drugs 

19465 

1 

C8 

Steroids 

19487 

1 

XB-C18 

7 2 5 

3 8 

4 

6 

App ID 19465 

3 

2 

5 

4 

6 

8 

7 

9 

App ID 19487 

4 5 

6 

7 min 

19488 

0 1 

2 3 4 5 min 

19466 

1 

2 

7 

5 

3 

4,8 

6 

C18 

App ID 19466 

1 

3 

2 

4 

5 

6 

8 

7 

9 

C18 

App ID 19488 

4 

5 6 7 min 

0 1 

2 

3 4 5 min 





Mobile Phase: A: 0.1% Phosphoric acid in Water 

B: 0.1% Phosporic acid in Acetonitrile 

Gradient: Time (min) 

% B 

0 30 

4 50 

7.5 70 




Sample: 

1. Naproxen 

2. Fenoprofen 

3. Ibuprofen 

4. Phenylbutazone 

Time (min) 

% B 

9 95 

10 30 

5. Indomethacin 

6. Meclofenamic acid 

7. Matrix impurity 

8. Matrix impurity 


Column: Kinetex 2.6 µm XB-C18 






0 20 

6 60 




Sample: 1. Estriol 

2. Hydrocortisone 

3. Cortisone 

4. Estradiol 

5. Cortisone-21-acetate 

18522 


6.01 20 

8 20 


7. Estrone 


9. Deoxycorticosterone acetate 

18605 

Norepinephrine and Epinephrine 

2 

HILIC 

Explosives 

18564 

3 

4 

PFP 

Resorcinol 

C18 

18604 

1 

2 

1 

1 

App ID 18605 

2 3 min 

C18 

App ID 18604 

18563 

1 

1 

2 

2 3 4 5 6 min 

4 

3 

7 

5 6 

5 

6 

7 

1 

2 

App ID 18564 

C18 

App ID 18563 

3 

1 

2 

5 

6 

4 

18521 

0 0.5 1 1.5 2 2.5 

2,3 

1 

5 

4 

App ID 18521 App ID 18522 

min 

PFP 

1 2 3 min 

1 

2 3 4 5 6 min 

0 0.5 1 1.5 2 2.5 

min 


Column: Kinetex 2.6 µm HILIC 



Mobile Phase 

(HILIC): 

Acetonitrile / 100 mM 

Ammonium formate 

pH 3.2 (92:8) 

Mobile Phase (C18): 5 mM Ammonium 

formate pH 3.2 / 

Methanol (97:3) 




Sample: 1. Norepinephrine 

2. Epinephrine 


Column: Kinetex 2.6 µm PFP 


Dimensions: 100 x 4.6mm 


B: Methanol 

Gradient: (45:55) A/B to (35:65) A/B 

over 6 min 




Sample: 1. HMX 

2. RDX 

3. 1,3,5-Trinitrobenzene 

4. 1,3-Dinitrobenzene 

5. Nitrobenzene 

6. 2,4,6-Trinitrotoluene 

7. 2,4-Dinitrotoluene 


Column: Kinetex 2.6 µm PFP 



Mobile Phase: 0.1 % Formic acid in Water / 

0.1 % Formic acid in Acetonitrile 

(85:15) 




Sample: 1. Resorcinol 

2. 2-Methylresorcinol 

3. Catechol 

4. 2,5-Dimethylresorcinol 

5. 4-Methylcatechol 

6. 4-Nitrocatechol 



33

Kinetex 

HPLC/UHPLC 


Choosing the 

Best Kinetex Column 

Expected Backpressure at Different Flow Rates* 

There is an optimal Kinetex column for your system and operating conditions. 

Use these graphs to determine the starting Kinetex particle size and dimension 

for your method. 

50:50 (Acetonitrile:Water) 

60:40 (Methanol:Water) 

600 

600 

Pressure (bar) 

500 

400 

300 

200 

UHPLC 

System 

Pressure (bar) 

500 

400 

300 

200 

UHPLC 

System 

100 

HPLC 

System 

0 

0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 min 

Flow Rate (mL/min) 

100 

HPLC 

System 

0 

0.0 0.5 1.0 1.5 2.0 2.5 3.0 min 

Flow Rate (mL/min) 

Kinetex 100 x 2.1 mm, 1.7 µm 








Select the right Kinetex 

column ID and flow rate (mL/min) 

4.6 

3.0 

2.1 

0 0.5 1.0 1.5 2.0 

Optimum 

0 0.5 1.0 1.5 2.0 

Optimum 

0 0.5 1.0 1.5 2.0 

Optimum 

Faster 

2.5 3.0 mL/min 

Faster 

2.5 

Faster 

2.5 

mL/min 

mL/min 

KINETEX CALCULATOR! 

Instantly optimize your method at 

www.phenomenex.com/optimize OR 

contact Your Phenomenex representative 

for optimization assistance. 


Phenomenex

Kinetex 

HPLC/UHPLC 



2.6 μm Analytical Columns (mm) KrudKatcher Ultra 

Phases 30 x 4.6 50 x 4.6 75 x 4.6 100 x 4.6 150 x 4.6 

/3pk 

XB-C18 –– 00B-4496-E0 00C-4496-E0 00D-4496-E0 00F-4496-E0 AF0-8497 

C18 00A-4462-E0 00B-4462-E0 00C-4462-E0 00D-4462-E0 00F-4462-E0 AF0-8497 

C8 –– 00B-4497-E0 00C-4497-E0 00D-4497-E0 00F-4497-E0 AF0-8497 

PFP 00A-4477-E0 00B-4477-E0 00C-4477-E0 00D-4477-E0 00F-4477-E0 AF0-8497 

HILIC –– 00B-4461-E0 00C-4461-E0 00D-4461-E0 00F-4461-E0 AF0-8497 

2.6 μm Solvent Saver MidBore Columns (mm) KrudKatcher Ultra 

Phases 30 x 3.0 50 x 3.0 75 x 3.0 100 x 3.0 150 x 3.0 

/3pk 

XB-C18 –– 00B-4496-Y0 –– 00D-4496-Y0 –– AF0-8497 

C18 00A-4462-Y0 00B-4462-Y0 00C-4462-Y0 00D-4462-Y0 00F-4462-Y0 AF0-8497 

C8 –– 00B-4497-Y0 –– 00D-4497-Y0 –– AF0-8497 

PFP 00A-4477-Y0 00B-4477-Y0 00C-4477-Y0 00D-4477-Y0 00F-4477-Y0 AF0-8497 

HILIC –– –– –– –– 00F-4461-Y0 AF0-8497 

2.6 μm Minibore Columns (mm) KrudKatcher Ultra 

Phases 30 x 2.1 50 x 2.1 100 x 2.1 150 x 2.1 

/3pk 

XB-C18 00A-4496-AN 00B-4496-AN 00D-4496-AN 00F-4496-AN AF0-8497 

C18 00A-4462-AN 00B-4462-AN 00D-4462-AN 00F-4462-AN AF0-8497 

C8 00A-4497-AN 00B-4497-AN 00D-4497-AN 00F-4497-AN AF0-8497 

PFP 00A-4477-AN 00B-4477-AN 00D-4477-AN 00F-4477-AN AF0-8497 

HILIC –– 00B-4461-AN 00D-4461-AN 00F-4461-AN AF0-8497 

1.7 μm Minibore Columns (mm) KrudKatcher Ultra 

Phases 50 x 2.1 100 x 2.1 150 x 2.1 

/3pk 

XB-C18 00B-4498-AN 00D-4498-AN –– AF0-8497 

C18 00B-4475-AN 00D-4475-AN 00F-4475-AN AF0-8497 

C8 00B-4499-AN 00D-4499-AN –– AF0-8497 

PFP 00B-4476-AN 00D-4476-AN 00F-4476-AN AF0-8497 

HILIC 00B-4474-AN –– –– AF0-8497 

The KrudKatcher Ultra: 

• Fits virtually all UHPLC / HPLC columns 

1.0 to 4.6 mm ID 

• Pressure rated to 20,000 psi (1,375 bar) 

• Extremely low dead volume minimizes sample peak dispersion 

The KrudKatcher Ultra filter body houses an integrated 0.5 µm 

316 stainless steel depth filter that efficiently removes microparticulates 

from the flow stream without contributing to system 

backpressure or dead volume (

Gemini ® 

HPLC 


Gemini-NX C18 

• Control selectivity of ionizable compounds for 

optimized methods 

• Consistent performance in both volatile and non-volatile 

buffers 

• High sample loading capacity for metabolite identification 

and preparative purification 

• pH stable 1-12 for durability 

Gemini-NX C18 

USP: L1 

LC/MS 

Certified 

Hydrophobicity 

H-Bonding 

Aromatic Selectivity 

Low 

Silanol Activity (pH 2.5) 

see p. 376 for testing probes. 

pH Stability 1.0-12.0 

Particle Size 3, 5, and 10 μm 

Phase C18 

Application Small Molecules, Basic Compounds 

Strength Most durable Hybrid Particle 

High 


Packing Material 

Particle 

Shape/Size 

(µm) 

Pore 

Size 

(Å) 

Surface 

Area 

(m 2 /g) 

Carbon 

Load 

% 

End 

Capping 

Gemini-NX C18 Spherical 3, 5,10 110 375 14 TMS 

Gemini-NX Tested for Extreme Durability in 

Changing Mobile Phase pH 

Column Efficiencies Maintained in High pH Testing for 20 Cycles 

% of Initial Efficiencies 

Retention in Minutes 

110 

100 

90 

80 

70 

60 

50 

0 5 10 15 20 

6 

5 

4 

3 

2 

1 

Diphenhydramine 

Tetracaine 

Stable Efficiencies After 

Exposure to 10,000 

Column Volumes of High and 

Low pH Buffers 

Cycles of Exposure 

Retention Times of Four Probes Maintained in Neutral pH 

Testing for 20 Cycles 

Retention Times Maintained 

After Exposure to 10,000 

Column Volumes of High and 

Low pH Buffers 

0 

0 5 10 15 20 

Acetophenone 

Benzene 

Toluene 

Acenaphthene 

Sequence 

Column Used: 

Column: Gemini-NX 5 µm C18 


Part No.: 00F-4454-E0 

Step 1 

24x High pH Flush Procedures 

Mobile Phase: A: 10 mM Ammonium 

Bicarbonate pH 10.5 


Gradient: 5 % to 95 % B in 6 min 

Hold at 95 % B for 2 min 

Re-equilibrate: 5 % B for 2 min 


Step 2 

High pH Testing 

Isocratic: 10 mM Ammonium 

Bicarbonate pH 10.5 / 

Acetonitrile (50:50) 



Samples: 1. Tetracaine 

2. Diphenhydramine 

Step 3 

1x Neutral Flush Procedure 



Gradient: 5 % B for 2 min 

5 % to 100 % B in 3 min 



Step 4 

Neutral pH Testing 

Isocratic: Water / Acetonitrile 

(35:65) 



Samples: 1. Acetophenone 

2. Benzene 

3. Toluene 

4. Acenaphthene 

Step 5 

24x Low pH Flush Procedure 

Mobile Phase: A: 0.5 % Formic Acid in Water 

B: 0.5 % Formic Acid in 

Acetonitrile, pH 2.0 

Gradient: 5 % to 95 % B in 6 min 


Re-equilibrate: 5 % B for 2 min 


Step 6 

Neutral pH Flush Repeats 

Repeats for 20 Cycles 


Phenomenex

Gemini ® 

HPLC 


Second-Generation TWIN-NX 

Technology 

TWIN-NX technology uses an improved patented organo-silica 

grafting process which incorporates highly stabilizing ethane 

cross-linking. These organic groups are evenly incorporated into 

the grafted layers on the silica surface while maintaining a pure 

silica core. This not only provides resistance to high pH attack, 

but also maintains the high efficiency and mechanical strength of 

a silica particle. 

O O O 

O O O 

O Si CH2 CH2 

Si O Si S 

CH2 

CH2 

Si CH2 CH2 

O 

Si O Si Si 

CH2 

CH2 

O O O O 

O O O O 

CH2 

C 

CH2 

Si O Si CH2 Si O Si CH2 

CH2 

CH2 

CH2Si 

O Si Si O Si 

CH2 

CH2 

C 

O O O O 

O O O O C 

CH2 

CH2 

O Si Si O Si Si CH2 

CH2 

CH2 

CH2 

CH2 

O Si Si O Si Si 

CH2 

CH2 

O O O 

O O O 


3 μm Microbore, Minibore and Narrow Bore Columns (mm) SecurityGuard Cartridges (mm) 

Phases 50 x 1.0 20 x 2.0 30 x 2.0 50 x 2.0 100 x 2.0 150 x 2.0 50 x 3.0 100 x 3.0 150 x 3.0 4 x 2.0* 

/10pk 

NX C18 — 00M-4453-B0 00A-4453-B0 00B-4453-B0 00D-4453-B0 00F-4453-B0 00B-4453-Y0 00D-4453-Y0 00F-4453-Y0 AJ0-8367 

for ID: 2.0-3.0 mm 

3 μm Analytical Columns (mm) SecurityGuard Cartridges (mm) 

Phases 20 x 4.0 30 x 4.6 50 x 4.6 100 x 4.6 150 x 4.6 250 x 4.6 4 x 3.0* 

/10pk 

NX C18 — — 00B-4453-E0 00D-4453-E0 00F-4453-E0 00G-4453-E0 AJ0-8368 

for ID: 3.2-8.0 mm 

5 μm Minibore and Narrow Bore Columns (mm) SecurityGuard Cartridges (mm) 

Phases 30 x 2.0 50 x 2.0 150 x 2.0 250 x 2.0 50 x 3.0 100 x 3.0 150 x 3.0 250 x 3.0 4 x 2.0* 

/10pk 

NX C18 00A-4454-B0 00B-4454-B0 00F-4454-B0 — 00B-4454-Y0 00D-4454-Y0 00F-4454-Y0 00G-4454-Y0 AJ0-8367 

for ID: 2.0-3.0 mm 

5 μm Analytical Columns (mm) SecurityGuard Cartridges (mm) 

Phases 30 x 4.6 50 x 4.6 100 x 4.6 150 x 4.6 250 x 4.6 4 x 3.0* 

/10pk 

NX C18 — 00B-4454-E0 00D-4454-E0 00F-4454-E0 00G-4454-E0 AJ0-8368 

for ID: 3.2-8.0 mm 

For Gemini Capillary HPLC Columns, 

Guards, and Adapter, contact your 

Phenomenex technical consultant or 

local distributor. 

5 μm Semi-Prep Columns (mm) SecurityGuard Cartridges (mm) 

Phases 150 x 10 250 x 10 10 x 10 ‡ 

/3pk 

NX C18 00F-4454-N0 00G-4454-N0 AJ0-8369 

for ID: 9-16 mm 

Axia Packed Preparative Columns (mm) 

SecurityGuard Cartridges (mm) 

Phases 50 x 21.2 100 x 21.2 150 x 21.2 250 x 21.2 50 x 30 75 x 30 15 x 21.2** 15 x 30.0 

5 μm /ea /ea 

NX C18 00B-4454-P0-AX 00D-4454-P0-AX 00F-4454-P0-AX 00G-4454-P0-AX 00B-4454-U0-AX 00C-4454-U0-AX AJ0-8370 AJ0-8371 

10 μm /ea /ea 

NX C18 00B-4455-P0-AX 00D-4455-P0-AX 00F-4455-P0-AX 00G-4455-P0-AX — — AJ0-8370 AJ0-8371 

for ID: 18-29 mm 30-49 mm 

Axia Packed Preparative Columns (mm) continued 

SecurityGuard Cartridges (mm) 

Phases 100 x 30 150 x 30 250 x 30 50 x 50 100 x 50 150 x 50 250 x 50 15 x 30.0 ♦ 

5 μm — — — /ea 

NX C18 00D-4454-U0-AX 00F-4454-U0-AX 00G-4454-U0-AX 00B-4454-V0-AX — — — AJ0-8371 

10 μm /ea 

NX C18 00D-4455-U0-AX 00F-4455-U0-AX 00G-4455-U0-AX 00B-4455-V0-AX 00D-4455-V0-AX 00F-4455-V0-AX 00G-4455-V0-AX AJ0-8371 

for ID: 30-49 mm 

Gemini is a registered trademark of Phenomenex, Inc. 

Twin-NX is a trademark of Phenomenex, Inc. 

*SecurityGuard Analytical Cartridges require holder, Part No.: KJ0-4282 

‡ 

SemiPrep SecurityGuard Cartridges require holder, Part No.: AJ0-7220 

**PREP SecurityGuard Cartridges require holder, Part No.: AJ0-8223 

PREP SecurityGuard Cartridges require holder, Part No.: AJ0-8277 


37

Synergi 

HPLC 

Full Range Selectivity for Reversed 

Phase Separation 

Synergi Polar-RP 

Phenyl 

Ether-Linked 

For polar and aromatic mixtures 

Synergi Fusion-RP 

C18 

Polar Embedded 

Balanced non-polar and polar performance 

Ether linkage increases aromaticity 

of the phenyl group and also provides π-π 

interactions with conjugated compounds 

Polar endcapping provides added 

retention for polar compounds 

Aq 

Aq 

Embedded polar group 

complements C18 ligand with 

balanced polar selectivity 

Aq 

TMS 

TMS endcapping 

ensures sharp peaks 

Aq 

TMS 

Ultra-pure Silica 


Synergi Hydro-RP 

C18 

Polar Endcapped 

Strong non-polar and polar retention 

Synergi Max-RP 

C12 

TMS Endcapped 

Excellent for basic compounds at neutral pH 

Polar endcapping provides added 

retention for polar compounds 

High density ligands and extensive 

endcapping ensure sharp peaks 

Aq 

Aq 

TMS 

TMS 




Particle 

Shape/Size 

(µm) 

Pore 

Size 

(Å) 

Pore 

Volume 

(mL/g) 

Surface 

Area 

(m 2 /g) 


Phenomenex 

Carbon 

Load 

% 

Calculated 

Bonded Phase 

Coverage 

(μmole/m 2 ) 

End 

Capping 

pH 

Ranges 


USP 

Synergi Max-RP Spher. 2.5 100 — 400 17 — TMS 1.5-10.0 

Synergi Hydro-RP Spher. 2.5 100 — 400 19 — Hydrophilic 1.5-7.5 L1 

Synergi Polar-RP Spher. 2.5 100 — 400 11 — Hydrophilic 1.5-7.0 L11 

Synergi Fusion-RP Spher. 2.5 100 — 400 12 — TMS 1.5-10.0 L1 

Synergi Max-RP Spher. 4, 10 80 1.05 475 17 3.21 TMS — — 

Synergi Hydro-RP Spher. 4, 10 80 1.05 475 19 2.45 Hydrophilic — — 

Synergi Polar-RP Spher. 4, 10 80 1.05 475 11 3.15 Hydrophilic — — 

Synergi Fusion-RP Spher. 4, 10 80 1.05 475 12 N/A TMS — —

Synergi 

HPLC 

Fast LC Solutions 


2.5 µm High Speed Technology (HST) Columns (mm) 

Phases 30 x 2.0 50 x 2.0 100 x 2.0 50 x 3.0 100 x 3.0 50 x 4.6 

Max-RP 

Hydro-RP 

Polar-RP 

Fusion-RP 

00A-4372-B0 

00A-4387-B0 

00A-4371-B0 

00A-4423-B0 

00B-4372-B0 

00B-4387-B0 

00B-4371-B0 

00B-4423-B0 

00D-4372-B0 

00D-4387-B0 

00D-4371-B0 

00D-4423-B0 

00B-4372-Y0 

00B-4387-Y0 

00B-4371-Y0 

00B-4423-Y0 

00D-4372-Y0 

00D-4387-Y0 

00D-4371-Y0 

00D-4423-Y0 

00B-4372-E0 

00B-4387-E0 

00B-4371-E0 

00B-4423-E0 

For information about HST Columns, contact your Phenomenex technical consultant or local distributor. 


2.5 µm MercuryMS LC/MS Cartridges (mm) Columns (mm) 

Phases 10 x 2.0 10 x 4.0 20 x 2.0 20 x 4.0 20 x 2.0 20 x 4.0 

Max-RP 00N-4372-B0-CE 00N-4372-D0-CE 00M-4372-B0-CE 00M-4372-D0-CE 00M-4372-B0 00M-4372-D0 

Hydro-RP 00N-4387-B0-CE 00N-4387-D0-CE 00M-4387-B0-CE 00M-4387-D0-CE 00M-4387-B0 00M-4387-D0 

Polar-RP — 00N-4371-D0-CE 00M-4371-B0-CE 00M-4371-D0-CE 00M-4371-B0 00M-4371-D0 

Fusion-RP 00N-4423-B0-CE 00N-4423-D0-CE 00M-4423-B0-CE 00M-4423-D0-CE 00M-4423-B0 00M-4423-D0 

Mercury MS Cartridge Holders 


Direct-Connect Cartridge Holders 

Part No. Description 

CH0-7187 10 mm direct-connect holder 

CH0-7188 20 mm direct-connect holder 

Direct-Connect Holder 

Capillary Columns 

Standard Holder 

Standard Cartridge Holders 

Part No. Description 

CH0-5846 10 mm standard holder 

CH0-5845 20 mm standard holder 


4 μm Synergi Capillary Columns (mm) 

Phases 50 x 0.30 150 x 0.30 250 x 0.30 50 x 0.50 150 x 0.50 250 x 0.50 

Max-RP 00B-4337-AC 00F-4337-AC — — 00F-4337-AF 00G-4337-AF 

Hydro-RP 00B-4375-AC 00F-4375-AC 00G-4375-AC 00B-4375-AF 00F-4375-AF 00G-4375-AF 

Fusion-RP 00B-4424-AC 00F-4424-AC — 00B-4424-AF 00F-4424-AF 00G-4424-AF 

4 μm Synergi Capillary Guard Columns (mm) 

Phases 20 x 0.30 20 x 0.50 

Max-RP 03M-4337-AC 03M-4375-AF 

Hydro-RP 03M-4375-AC 03M-4375-AF 

Fusion-RP 03M-4424-AC — 

Analytical Columns 


4 µm Microbore and Minibore Columns (mm) SecurityGuard Cartridges (mm) 

Phases 50 x 1.0 150 x 1.0 250 x 1.0 30 x 2.0 50 x 2.0 75 x 2.0 150 x 2.0 250 x 2.0 4 x 2.0* 

/10pk 

Max-RP 00B-4337-A0 00F-4337-A0 — 00A-4337-B0 00B-4337-B0 00C-4337-B0 00F-4337-B0 00G-4337-B0 AJ0-6073 

Hydro-RP 00B-4375-A0 00F-4375-A0 00G-4375-A0 00A-4375-B0 00B-4375-B0 00C-4375-B0 00F-4375-B0 00G-4375-B0 AJ0-7510 

Polar-RP 00B-4336-A0 00F-4336-A0 — 00A-4336-B0 00B-4336-B0 00C-4336-B0 00F-4336-B0 00G-4336-B0 AJ0-6075 

Fusion-RP 00B-4424-A0 00F-4424-A0 — 00A-4424-B0 00B-4424-B0 00C-4424-B0 00F-4424-B0 00G-4424-B0 AJ0-7556 

for ID: 2.0-3.0 mm 

4 µm Narrow Bore Columns (mm) SecurityGuard Cartridges (mm) 

Phases 30 x 3.0 50 x 3.0 150 x 3.0 250 x 3.0 4 x 2.0* 

/10pk 

Max-RP 00A-4337-Y0 00B-4337-Y0 00F-4337-Y0 00G-4337-Y0 AJ0-6073 

Hydro-RP 00A-4375-Y0 00B-4375-Y0 00F-4375-Y0 00G-4375-Y0 AJ0-7510 

Polar-RP 00A-4336-Y0 00B-4336-Y0 00F-4336-Y0 00G-4336-Y0 AJ0-6075 

Fusion-RP 00A-4424-Y0 00B-4424-Y0 00F-4424-Y0 00G-4424-Y0 AJ0-7556 

for ID: 2.0-3.0 mm 

4 µm Analytical Columns (mm) SecurityGuard Cartridges (mm) 

Phases 30 x 4.6 50 x 4.6 75 x 4.6 150 x 4.6 250 x 4.6 4 x 3.0* 

/10pk 

Max-RP 00A-4337-E0 00B-4337-E0 00C-4337-E0 00F-4337-E0 00G-4337-E0 AJ0-6074 

Hydro-RP 00A-4375-E0 00B-4375-E0 00C-4375-E0 00F-4375-E0 00G-4375-E0 AJ0-7511 

Polar-RP 00A-4336-E0 00B-4336-E0 00C-4336-E0 00F-4336-E0 00G-4336-E0 AJ0-6076 

Fusion-RP 00A-4424-E0 00B-4424-E0 00C-4424-E0 00F-4424-E0 00G-4424-E0 AJ0-7557 

for ID: 3.2-8.0 mm 

Synergi Preparative columns are also available. Please contact your Phenomenex technical consultant or local Distributor. 


39

Luna ® 

HPLC 

One of the World’s Leading 


Develop or Improve your HPLC Method 

• Exceptionally rugged USP phases 

• Easy method scalability with 3, 5, 10, 10-PREP and 15 μm medias 

• Extensive batch traceability and reproducibility data supplied with every column 


Particle 

Shape/Size 

(μm) 

Pore 

Size 

(Å) 

Surface 

Area 

(m 2 /g) 

Carbon 

Load 

% 

Calculated Bonded 

Phase Coverage 

(µmole/m 2 ) 

End 

Capping 

pH 

Stability 


Luna Silica(2) Spher. 3, 5,10, 10-PREP, 15 100 400 0 – No 2.0 - 7.5 

Luna C5 Spher. 5,10 100 440 12.5 7.85 Yes 1.5 - 10* 

Luna C8(2) Spher. 3, 5,10, 10-PREP, 15 100 400 13.5 5.50 Yes 1.5 - 10* 

Luna C18(2) Spher. 2.5, 3, 5,10, 10-PREP, 15 100 400 17.5 3.00 Yes 1.5 - 10* 

Luna Phenyl-Hexyl Spher. 3, 5,10, 10-PREP, 15 100 400 17.5 4.00 Yes 1.5 - 10* 

Luna CN Spher. 3, 5, 10 100 400 7.0 3.80 Yes 1.5 - 7.0 

Luna NH 2 

Spher. 3, 5, 10 100 400 9.5 5.80 No 1.5 - 11 

Luna SCX Spher. 5,10 100 400 Binding Capacity: 0.15 meq/g No 2.0 - 7.0 

Luna HILIC Spher. 3, 5 200 200 5.7 4.30 No 1.5 - 8.0 

Luna PFP(2) Spher. 3, 5 100 400 11.5 2.20 Yes 1.5 - 8.0 

* pH range under isocratic conditions. pH range is 2 - 8 under gradient conditions. 

USP Phases for Virtually Every Application 

Luna Bonded Phase Selectivity Chart 

USP Column 

Classification Phase Description Common Applications 

L1 

L3 

L7 

L8 

L9 

L10 

L11 

L20 

L43 

C18(2) 2.5, 3, 5, 10, 10-PREP, 15 µm 

C18 phase. Excellent efficiency, peak shape and resolution. Slightly 

lower carbon load than original Luna C18. 

Silica(2) 3, 5, 10 µm 

Ultra-pure silica with high column bed stability enhanced by particle 

shape uniformity. 

C8(2) 3, 5, 10, 10-PREP, 15 µm 

C8 phase for excellent efficiency, peak shape and resolution. 

Significantly improved performance over traditional C8 phases due to 

high surface coverage. 

NH 2 

3, 5, 10 µm 

Amino phase. Can be used in reversed or normal phase modes. 

Stable from pH 1.5 to 11.0 and under 100% aqueous conditions. 

High performance silica and bonding techniques produces a rugged, 

highly reproducible column. 

SCX 5, 10 µm 

A Benzene Sulfonic Acid bonded phase is used to make this Strong 

Cation Exchange (SCX) column. Offers great peak shape and resolution. 

CN 3, 5, 10 µm 

Cyano phase. Can be used as reversed- or normal-phase material. 

The use of Luna base silica results in overall phase reproducibility and 

performance. 

Phenyl-Hexyl 3, 5, 10, 10-PREP, 15 µm 

A phenyl phase which employs a hexyl alkyl linker as opposed to the 

traditional propyl chain. Offers great stability as well as alternative 

selectivity. 

HILIC 3, 5 µm 

HILIC phase that provides excellent selectivity for polar compounds; and 

improved MS sensitivity with low bleed. 

PFP(2) 3, 5 µm 

A pentafluorophenyl phase that provides excellent selectivity for aromatic 

compounds from influence of flourine substitution on phenyl ring. Multiple 

retention mechanisms. Orthogonal selectivity to traditional C18 phases. 

Luna ® is a registered trademark of Phenomenex, Inc. 

Acetaminophen, Aspirin, Caffeine, Albuterol, Amitriptyline Hydrochloride, 

Amoxicillin, Atenolol, Cephalexin, Cephradine capsules, Chloramphenicol, 

Cortisone Acetate, Dextromethorphan, Diphenhydramine, Pseudoephedrine, 

Dopamine, Estradiol, Guaifenesin, Ibuprofen, Sterile Imipenem, Imipramine, 

Lidocaine, Lorazepam, Minoxidil, Naproxen, Phenylephrine Hydrochloride, 

Phenylpropanolamine, Prednisone oral solution, Procainamide, Propoxyphene, 

Reserpine 

Alprazolam, Hydrocodone bitartrate, Hydrocortisone, Fat Soluble Vitamins, 

Phthalates, Fatty Acids, Lutein, Lycopene, Estradiol 

Doxepine, Doxylamine succinate, Fluoxetine, Glyburide, Ibuprofen Oral 

Suspension, Propranolol, Levonorgestrel, Ethinyl estradiol, Melengestrol 

acetate, Glucosamine 

Simple sugars, Carboplatin, Lactulose concentrate, Levocarnitine tablets 

Cough and cold compounds, Raclopride, Sodium Acetate, Erythromycin 

Benzalkonium Chloride, Nortriptyline HCl Capsules, Prednisolone, Tetracaine, 

Quinapril tablets 

Oxacillin, Captopril, Chlorpheniramine, Pseudoephedrine, Methadone 

Hydrochloride Oral Concentration 

Drug metabolites, Water soluble vitamins, Melamine, Cyanuric acid, 

Metanephrine, Normetanephrine 

Positional isomers, Geometric isomers, Taxanes, Aflatoxins 

Try Gemini ® -NX for even longer column lifetimes (see page 36-37). 


Phenomenex

Luna ® 

HPLC 


3 μm Microbore and Minibore columns (mm) SecurityGuard Cartridges (mm) 

Phases 30 x 2.0 50 x 2.0 100 x 2.0 150 x 2.0 4 x 2.0 

/10pk 

Silica(2) 00A-4162-B0 00B-4162-B0 00D-4162-B0 00F-4162-B0 AJ0-4347 

C8(2) 00A-4248-B0 00B-4248-B0 00D-4248-B0 00F-4248-B0 AJ0-4289 

C18(2) 00A-4251-B0 00B-4251-B0 00D-4251-B0 00F-4251-B0 AJ0-4286 

CN 00A-4254-B0 00B-4254-B0 00D-4254-B0 00F-4254-B0 AJ0-4304 

Phenyl-Hexyl 00A-4256-B0 00B-4256-B0 00D-4256-B0 00F-4256-B0 AJ0-4350 

NH 2 

00A-4377-B0 00B-4377-B0 00D-4377-B0 00F-4377-B0 AJ0-4301 

HILIC — 00B-4449-B0 00D-4449-B0 00F-4449-B0 AJ0-8328 

PFP(2) 00A-4447-B0 00B-4447-B0 00D-4447-B0 00F-4447-B0 AJ0-8326 

for ID: 2.0-3.0 mm 

5 μm Microbore and Minibore columns (mm) SecurityGuard Cartridges (mm) 

Phases 150 x 2.0 250 x 2.0 4 x 2.0* 

/10pk 

Silica(2) 00F-4274-B0 00G-4274-B0 AJ0-4347 

C5 00F-4043-B0 — AJ0-4292 

C8(2) 00F-4249-B0 00G-4249-B0 AJ0-4289 

C18(2) 00F-4252-B0 00G-4252-B0 AJ0-4286 

CN 00F-4255-B0 00G-4255-B0 AJ0-4304 

Phenyl-Hexyl 00F-4257-B0 00G-4257-B0 AJ0-4350 

NH 2 

00F-4378-B0 00G-4378-B0 AJ0-4301 

PFP(2) 00F-4448-B0 — AJ0-8326 

for ID: 2.0-3.0 mm 

3 μm Narrow Bore and analytical columns (mm) SecurityGuard Cartridges (mm) 

Phases 30 x 3.0 50 x 3.0 150 x 3.0 30 x 4.6 50 x 4.6 75 x 4.6 100 x 4.6 150 x 4.6 4 x 2.0 4 x 3.0 

/10pk 

/10pk 

Silica(2) — — 00F-4162-Y0 — 00B-4162-E0 — 00D-4162-E0 00F-4162-E0 AJ0-4347 AJ0-4348 

C8(2) 00A-4248-Y0 00B-4248-Y0 00F-4248-Y0 00A-4248-E0 00B-4248-E0 00C-4248-E0 00D-4248-E0 00F-4248-E0 AJ0-4289 AJ0-4290 

C18(2) 00A-4251-Y0 00B-4251-Y0 00F-4251-Y0 00A-4251-E0 00B-4251-E0 00C-4251-E0 00D-4251-E0 00F-4251-E0 AJ0-4286 AJ0-4287 

CN — 00B-4254-Y0 00F-4254-Y0 00A-4254-E0 00B-4254-E0 00C-4254-E0 00D-4254-E0 00F-4254-E0 AJ0-4304 AJ0-4305 

Phenyl-Hexyl — 00B-4256-Y0 00F-4256-Y0 00A-4256-E0 00B-4256-E0 00C-4256-E0 00D-4256-E0 00F-4256-E0 AJ0-4350 AJ0-4351 

NH 2 

— 00B-4377-Y0 00F-4377-Y0 — 00B-4377-E0 — 00D-4377-E0 00F-4377-E0 AJ0-4301 AJ0-4302 

HILIC — 00B-4449-Y0 00F-4449-Y0 — — — 00D-4449-E0 00F-4449-E0 AJ0-8328 AJ0-8329 

PFP(2) — 00B-4447-Y0 00F-4447-Y0 — 00B-4447-E0 — 00D-4447-E0 00F-4447-E0 AJ0-8326 AJ0-8327 

for ID: 2.0-3.0 mm 

3.2-8.0 mm 

5 μm Narrow Bore and analytical columns (mm) SecurityGuard Cartridges (mm) 

Phases 30 x 3.0 50 x 3.0 150 x 3.0 250 x 3.0 30 x 4.6 50 x 4.6 75 x 4.6 4 x 2.0* 4 x 3.0* 

/10pk 

/10pk 

Silica(2) — — — — — 00B-4274-E0 — AJ0-4347 AJ0-4348 

C5 — — 00F-4043-Y0 — — 00B-4043-E0 — AJ0-4292 AJ0-4293 

C8(2) — 00B-4249-Y0 00F-4249-Y0 00G-4249-Y0 00A-4249-E0 00B-4249-E0 00C-4249-E0 AJ0-4289 AJ0-4290 

C18(2) 00A-4252-Y0 00B-4252-Y0 00F-4252-Y0 00G-4252-Y0 00A-4252-E0 00B-4252-E0 00C-4252-E0 AJ0-4286 AJ0-4287 

CN — 00B-4255-Y0 00F-4255-Y0 00G-4255-Y0 00A-4255-E0 00B-4255-E0 00C-4255-E0 AJ0-4304 AJ0-4305 

Phenyl-Hexyl 00A-4257-Y0 00B-4257-Y0 00F-4257-Y0 00G-4257-Y0 00A-4257-E0 00B-4257-E0 00C-4257-E0 AJ0-4350 AJ0-4351 

NH 2 

— 00B-4378-Y0 00F-4378-Y0 00G-4378-Y0 00A-4378-E0 00B-4378-E0 00C-4378-E0 AJ0-4301 AJ0-4302 

SCX — — — — — 00B-4398-E0 — AJ0-4307 AJ0-4308 

HILIC — — 00F-4450-Y0 — — — — AJ0-8328 AJ0-8329 

PFP(2) — 00B-4448-Y0 00F-4448-Y0 — 00A-4448-E0 00B-4448-E0 — AJ0-8326 AJ0-8327 

for ID: 2.0-3.0 mm 

3.2-8.0 mm 

5 μm Analytical columns (mm) SecurityGuard Cartridges (mm) 

Phases 100 x 4.6 150 x 4.6 250 x 4.6 4 x 3.0* 

/10pk 

Silica(2) 00D-4274-E0 00F-4274-E0 00G-4274-E0 AJ0-4348 

C5 00D-4043-E0 00F-4043-E0 00G-4043-E0 AJ0-4293 

C8(2) 00D-4249-E0 00F-4249-E0 00G-4249-E0 AJ0-4290 

C18(2) 00D-4252-E0 00F-4252-E0 00G-4252-E0 AJ0-4287 

CN 00D-4255-E0 00F-4255-E0 00G-4255-E0 AJ0-4305 

Phenyl-Hexyl 00D-4257-E0 00F-4257-E0 00G-4257-E0 AJ0-4351 

NH 2 

00D-4378-E0 00F-4378-E0 00G-4378-E0 AJ0-4302 

SCX 00D-4398-E0 00F-4398-E0 00G-4398-E0 AJ0-4308 

HILIC 00D-4450-E0 00F-4450-E0 00G-4450-E0 AJ0-8329 

PFP(2) 00D-4448-E0 00F-4448-E0 00G-4448-E0 AJ0-8327 

for ID: 3.2-8.0 mm 

Method development column kits and 

method validation column kits are 

available. Contact Phenomenx or your 

local distributor for details. 

Luna Preparative columns are also 

available. Contact Phenomenex or your 

local distributor for more information 

about Axia Preparative Columns. 



41

Lux ® 

Chiral 

HPLC Analysis 

Five Distinct Polysaccharide 

Phases to Resolve 92 % of 

Your Enantiomers* 

Lux columns offer a wide and complementary range of enantioselectivity 

for even the most difficult chiral separation projects. 

• High efficiency and loading capacity 

• Stable in Normal Phase, Polar Organic, SFC, and 

Reversed Phase Conditions 

• 3 µm and 5 µm packed columns and 20 µm bulk media for 

scale up † 

C-1 C-2 

Technical Specifications 

Particle Size 3, 5, 20* µm 


Maximum Pressure 300 bar 

Temperature Range 0-50 °C 

Shipping Solvent 

n-Hexane/2-propanol (9:1, v/v) 

† 

Please inquire for availability 

Lux chiral columns are made with high quality silica, and undergo 

a specialized coating and packing process that ensures high efficiencies 

while maintaining essential enantiorecognition abilities. 

H 

H 

C 

H 

H 

Lux Cellulose-1 

Cellulose tris(3,5-dimethylphenylcarbamate) 

C 

Cellulose-O-CONH 

H 

H 

C 

H 

H C H 


C-1 C-1 C-2 

C-2 

C-3 

C-3 

C-4 

C-4 

H 

H 

H 

H 

C 

H 

H 

C 

H 

H 

H 

H 

C 

H 

H 

C 

H 

H 

H 

H C H 

Cl 

H 

H C H 

Cl 

H 

C 

H 

H 

C 

H 

H 

H 

H 

C 

Cl 

H 

H 

H 

C 

Cl 

H 

O 

O 

H 

C 

H 


Cellulose-O-CONH Cellulose-O-CONH 


Cellulose-O 

Cellulose-O 


Cellulose-O-CONH Amylos 


Cellulose tris(3-chloro-4-methylphenylcarbamate) 


Cellulose tris(4-methylbenzoate) 

C-2 C-3 

C-3 

C-4 

C-4 

A-2 

A-2 

H 

H 

H 

H C H 

H C 

Cl 

H 

H 

C 

H 

C 

H 

H 

Cl 

H 

C 

H 

Cl 

Cl 

Cl 

H 

H 

H 

H 

O 

O 

H 

C 

H 

H 

C 

H 

Cellulose-O-CONH Cellulose-O 

Cellulose-O Cellulose-O-CONH 

Cellulose-O-CONH Amylose-O-CONH 

Amylose-O-CONH 


Cellulose tris(4-chloro-3-methylphenylcarbamate) 

Lux Amylose-2 

Amylose tris(5-chloro-2-methylphenylcarbamate) 

* Based on 233 compounds screened on all five Lux phases. 


Phenomenex

Lux ® 

Chiral 

HPLC Analysis 

Achieve Optimal Resolution by Screening all Five 

Complementary Lux Chiral Columns 

Utilizing differences in selectivity can help develop methods more efficiently 

by offering broad and contrasting chiral recognition abilities. 

Lux chiral selectors provide a variety of selectivities that give you the 

opportunity 19147 to screen for the ideal chiral separation. 

α = 1.06 

Etozolin 

19151 Lux 5 µm Cellulose-1 

α a = 1.06 

1.35 

1 2 

0 2 4 6 8 10 12 14 16 18 min 

Lux 5 µm Cellulose-4 

a = 1.35 

19146 

α = 1.45 

0 2 4 6 8 10 12 14 16 18 min 

Lux 5 µm Amylose-2 

a = 1.45 

19148 

α = 1.48 

1 

1 

2 

0 2 4 6 8 10 12 14 16 18 min 


a = 1.48 

19152 

α = 1.70 

0 2 4 6 8 10 12 14 16 18 min 

2 

1 

2 

App ID 19148 App ID 19146 

App ID 19151 App ID 19147 

Conditions for all columns: 


Mobile Phase: Acetonitrile / 0.1 % Diethylamine in 20 mM NH 4 

HCO 3 

(60:40) 

Flow Rate: 1 mL/min 



N 

O 

S 

N 

O 

O 

Optimal Resolution 

1 

2 


a = 1.70 

Based on a five phase screen under reversed phase 

conditions, the optimal chiral stationary phase for 

resolving Etozolin is Lux Cellulose-3. 

App ID 19152 

0 2 4 6 8 10 12 14 16 18 min 



43

Lux ® 

Chiral 

HPLC Analysis 



Phases 50 x 2.0 150 x 2.0 50 x 4.6 100 x 4.6 150 x 4.6 250 x 4.6 4 x 2.0* 4 x 3.0* 

/10pk 

/10pk 

Cellulose-1 00B-4458-B0 00F-4458-B0 00B-4458-E0 00D-4458-E0 00F-4458-E0 00G-4458-E0 AJ0-8402 AJ0-8403 




Amylose-2 00B-4471-B0 00F-4471-B0 00B-4471-E0 00D-4471-E0 00F-4471-E0 00G-4471-E0 AJ0-8471 AJ0-8470 

for ID: 2.0 –3.0 mm 3.2–8.0 mm 


Phases 50 x 2.0 50 x 4.6 100 x 4.6 150 x 4.6 250 x 4.6 4 x 2.0* 4 x 3.0* 

/10pk 

/10pk 

Cellulose-1 00B-4459-B0 00B-4459-E0 00D-4459-E0 00F-4459-E0 00G-4459-E0 AJ0-8402 AJ0-8403 




Amylose-2 00B-4472-B0 00B-4472-E0 00D-4472-E0 00F-4472-E0 00G-4472-E0 AJ0-8471 AJ0-8470 

for ID: 2.0–3.0 mm 3.2–8.0 mm 

5 µm Semi-Prep Columns (mm) 


Cartridges (mm) 

Phases 150 x 10.0 250 x 10.0 10 x 10.0 ‡ 

/3pk 

Cellulose-1 00F-4459-N0 00G-4459-N0 AJ0-8404 




Amylose-2 00F-4472-N0 00G-4472-N0 AJ0-8472 

for ID: 9–16 mm 

20 µm Bulk Media 

Phases 100 g 1 kg 

Inquire 

Inquire 

Cellulose-1 04G-4473 04K-4473 

Cellulose-2 04G-4464 04K-4464 

Please inquire for 20 µm Lux Amylose-2, Cellulose-3 or 

Cellulose-4 media. 

Lux Preparative columns are also available. Contact 

Phenomenex or your local distributor for more 

information on AXIA Preparative Columns. 

Chiral Screening and Separation Services 

HPLC | SFC | Preparative | Training 

For more information on our services or to begin a project, please 

contact your local Phenomenex chiral expert or email us at: 

phenologix@phenomenex.com 

You can also visit us on the web at: 

www.phenomenex.com/phenologix 


Phenomenex

BioSep -SEC-S 

GFC 

Size Exclusion 

Chromatography 

Aqueous Size Exclusion/ 

Gel Filtration for Protein and 

Peptide Analysis 

Gel Filtration Chromatography is used for the analysis and/or 

characterization of proteins, peptides and other biomolecules; 

including antibodies, immunoglobulins, protein complexes, protein 

aggregates, and desalting. BioSep GFC columns offer many 

important benefits for your separation needs. 

• Hydrophilically bonded rigid spherical silica gel has nominal 

adsorbtion and high sample recoveries 

• Narrow defined pore size distribution produces exceptional 

separations for SEC 

• Optimized packing specifications provide high resolution, 

maximized efficiencies, and exceptional peak asymmetry 

Batch-to-Batch Reproducibility 

Batch-to-batch and column-to-column reproducibility is imperative. 

Each batch of BioSep media is carefully monitored and QC 

tested to ensure proper size, shape and pore characteristics, 

which ensures the same high quality separation time and time 

18897 

again. 

mAU 

200 

150 

5 

6 

7 

8 

Molecular Weight Separation Range for Proteins on BioSep-SEC-S 

3 BioSep phase options to separate samples of varying molecular 

weight (MW) ranges: 2000, 3000, 4000, as described below: 

100 

3 Separate 

Batches Overlaid 

3 

4 

Under 

6 M GnHCI 

Under 

0.5 % SDS 

S-4000 5,000 - 700,000 

S-3000 1,000 - 150,000 

S-2000 500 - 100,000 

S-2000 200 - 75,000 

S-4000 15,000 - 500,000 

S-3000 5,000 - 100,000 

50 

2 

1 

0 

0 2 4 6 8 10 12 14 min 

App ID 18897 

Native 

S-4000 15,000 - 1,500,000 

S-3000 5,000 - 700,000 

S-2000 1,000 - 300,000 

102 103 104 105 106 107 

Molecular Weight (Daltons) 

High Efficiencies for Greater Resolution 

Due to a tight particle size distribution and a specialized packing 

process and specifications, you will see greater baseline separation 

between your analytes. 

App ID 18927 

Highly Inert Material for Better Recovery 

and Quantitation 

BioSep columns experience a nominal amount of non-specific interactions 

which provides an extremely inert media demonstrating 

distinct advantages for accurate quantitation of your proteins 

18966 

and aggregates. 

Human Serum under Different Mobile Phases 

Efficiency 

(minimum number theoretical plates on 300 x 7.8 mm column) 

1. Mobile phase containing NaCl 

SEC-s2000 

30,000 

Plates 

SEC-s3000 

30,000 

Plates 

SEC-s4000 

25,000 

Plates 

2. Mobile phase containing Arginine 

3. Mobile phase without Arginine 

App ID 18966 

0 2 

4 6 8 10 12 14 min 

Equal recovery of proteins and aggregates under different mobile phase conditions is 

indicative of a highly inert column. 


45

BioSep -SEC-S 

GFC 



Low MW Proteins and Peptides on 

BioSep-SEC-s2000 

BioSep-SEC-s2000 columns are used for peptide therapeutics, 

small proteins, PEGylated peptides and small PEGylated proteins, 

as well as biogeneric aggregate applications. 

Medium MW Proteins on 


BioSep-SEC-s3000 columns are great for medium to large MW 

proteins, serum proteins, and immunoglobulin aggregate and 

applications. 

Low MW Protein and Peptide Mixture 

Column: BioSep-SEC-s2000 


Part No.: 00H-2145-K0 

Mobile 45 % Acetonitrile, 0.1 % TFA 

Phase: 



18945 


Sample: 

Protein Mixture 



18928 

Part No.: 00H-2146-K0 

Mobile Phase: 100 mM Sodium Phosphate pH 7.0, 

300 mM Sodium Chloride 



1 


Sample: 1. Thyroglobulin 669 kDa 

2. lgG 156 kDa 

3. BSA 66 kDa 

4. Ovalbumin 45 kDa 

5. Myoglobin 16.9 kDa 

6. Uridine 244 Da 

5 

6 

3 

4 

14. L-Glutathione 307 Da 

13. Leu-Leu-Leu 358 Da 

2 

12. Bradykinin 1-5 573 Da 

11. Bradykinin 1-7 760 Da 

10. Angiotensin 1-7 899 Da 

9. Angiotensin II 1046 Da 

8. Bradykinin 1060 Da 

7. Substance P 1-9 1104 Da 

6. Angiotensin I 1297 Da 

5. Glucagon 3482 Da 

App ID 18928 

4. Insulin 5700 Da 

3. Apotinin 6500 Da 

2. Cytochrome c 12400 Da 

1. Myoglobin 17800 Da 

App ID 18945 

4 6 8 10 12 

min 

0 2 4 6 8 10 

Protein Separation of 50-300,000 MW on BioSep-SEC-s2000 

vs. TSKgel ® G2000SW XL 


Columns: BioSep-SEC-s2000 

TSKgel ® G2000SW XL 


18892 Mobile 10 mM Tris pH 7.4, 

Phase: 150 mM Sodium Chloride 


mAU 

400 

300 

All four proteins 

are resolved 




Sample: 1. Hu IgA 300 kDa 

2. b-Amylase 200 kDa 

3. BSA 66 kDa 

4. Ovalbumin 45 kDa 

1 

2 

3 

4 


min 

Large MW Proteins on 


BioSep-SEC-s4000 is capable of resolving large MW proteins 

and PEGylated IgG applications. 

High MW Protein Mixture 



Part No.: 00H-2147-K0 

Mobile 100 mM Sodium Phosphate pH 7.0, 

Phase: 300 mM Sodium Chloride 




Sample: 1. HMW impurity 

18936 

2. lgM 900 kDa 

3. Thyroglobulin 669 kDa 

4. lgA 300 kDa 

5. b-Amylase 200 kDa 

6. BSA 66 kDa 

7. Ribonuclease A 13.7 kDa 

8. Uridine 244 Da 

5 

6 

7 

8 

200 

1,2 

3 

TSKgel ® 

G2000SW XL 

3 

4 

100 

Co-migration 

of peaks 1 & 2 

4 

App ID 18892 

1 

2 

App ID 18936 

0 

0 5 10 15 

min 

0 2 4 6 8 10 12 

14 

min 


Phenomenex

BioSep -SEC-S 

GFC 



• Global support and availability in over 65 countries 

• 3 batches available for validation 

• Large inventory for immediate shipment 

Technical Data and Specifications 

BioSep SEC-s2000 BioSep SEC-s3000 BioSep SEC-s4000 

Resin Type Silica Silica Silica 

Particle Size (µm) 5 5 5 

Pore Size (Å) 145 290 500 

pH Range 2.5 - 7.5 2.5 - 7.5 2.5 - 7.5 

Maximum Backpressure (psi) 1,500 1,500 1,500 

Typical Backpressure (psi) 800 800 700 

Efficiency 

30,000 30,000 25,000 

(minimum number theoretical plates 300 x 7.8 mm) 

Maximum Flow Rate 

This is a function of pressure. Columns can withstand up to 1,500 psi, but avoid sudden pressure changes. 

Column Hardware 

Standard: 316 stainless steel column with stainless steel frits. Titanium frits available. 

Maximum Temp. 

50 ˚C 

Maximum Salt Conc. 

1 M 

Denaturants 

0.5 % SDS, 6 M Guanidine HCI, or 8 M urea 

Regeneration 

After exposure to denaturants, wash with water overnight. 

Max. Organic Modifier 

Up to 100 % CH 3 

CN. Start with 100 % H 2 

O, linear gradient to 100 % CH 3 

CN over 50 min. 

Up to 90 % CH 3 

CN, 10 % DMSO or 500 mM b-mercaptoethanol. 

Cleaning Procedure General protein removal: wash with 30 mL of 0.1 M NaH 2 

P0 4 

, pH 3.0. 

Hydrophobic protein removal: use acetonitrile gradient. 

Strongly adsorbed proteins: wash with 30 mL of 0.5 % SDS or 6 M Guanidine thiocyanate or 10 % DMSO. 

Storage Overnight storage: run mobile phase at 0.2 mL/minute. Prolonged storage: use 0.05 % NaN 3 

in H 2 

O or 10 % methanol in H 2 

0. 

Column Protection 

Use of a SecurityGuard is recommended to prolong column lifetime. 

Cross Reference Chart 

Phenomenex 

Waters ® 

BioSep Phases TSK-Gel ® Shodex ® Sepax Bio-Rad ® BioSuite Zorbax ® 

SEC-s2000 G2000SW PROTEIN KW-802.5 SRT ® -100* Bio-Sil ® SEC 125 BioSuite 125 GF-250 

G2000SW XL 

SEC-s3000 

SEC-s4000 

SRT ® -150 

G3000SW PROTEIN KW-803 SRT ® -300 Bio-Sil ® SEC 250 BioSuite 250 GF-450 

G3000SW XL 

G4000SW PROTEIN KW-804 SRT ® -500** Bio-Sil ® SEC 400 BioSuite 450** 

G4000SW XL 

** Only up to 1,500,000 MW 

* Only above 1,000 MW 


*SecurityGuard Analytical cartridges require holder, Part No.: KJ0-4282 

** PREP SecurityGuard Cartridges require holder, Part No.: AJ0-8223 

Stainless Steel Columns (mm): Narrow Bore Analytical Preparative SecurityGuard Cartridges (mm) 

Phases 300 x 4.6 300 x 7.8 600 x 7.8 300 x 21.2 4 x 3.0* 15 x 21.2** 

/10pk 

ea 

BioSep-SEC-s2000 00H-2145-E0 00H-2145-K0 00K-2145-K0 00H-2145-P0 AJ0-4487 AJ0-8588 



for ID: 4.6-7.8 mm for ID: 21.2 mm 

Stainless Steel Guard Columns (mm) Narrow Bore Express Analytical 

Phases 30 x 4.6 35 x 7.8 75 x 7.8 

BioSep-SEC-s2000 03A-2145-E0 03Q-2145-K0 03C-2145-K0 



Aqueous SEC 1 Column Check Standard 

(for BioSep-SEC-S and other protein SEC columns) 

Part No.: AL0-3042 

Unit quantity: Dry; reconstituted to 2 mL 

Contains: Bovine thyroglobulin; Human gamma globulin; 

Ovalbumin; Myoglobin; Uridine (reconstitute 

with 1 mL of 100 mM Sodium phosphate pH 6.8) 

Diluent: 100 mM Sodium phosphate pH 6.8 

Storage: Add 0.1 % NaN 3 

to the solution and refrigerate 

Test Conditions 

Mobile phase: 100 mM Sodium phosphate, pH 6.8 

Flow rate: 1.0 mL/min for a 300 x 7.8 mm column 

Injection volume: 10 µL 


Other column dimensions available upon request. 


47

Jupiter ® 

HPLC 


RP-HPLC for Protein/Peptide Analysis 

and Purification 

The Jupiter HPLC column portfolio, including Jupiter 300 and Jupiter Proteo, offers optimized 

reversed phase solutions for protein and peptide characterization and purification. With these columns, 

one can identify, purify, and analyze almost any protein. 

Jupiter 300 – 300 Å columns designed to analyze and purify intact proteins 

• For separation of intact proteins > 10,000 MW 

• Available with C18, C5, and C4 bonded phases 

• 1.5 – 10 pH stability for method ruggedness and easy protein removal 

• Direct scale-up to preparative and bulk materials 

Jupiter Proteo – 90 Å columns engineered for increased peak capacity and resolution of 

peptide maps as well as peptide separations 

• For separation of intact proteins and peptides < 10,000 MW 

• Available with novel C12 bonded phase for excellent selectivity 

• Identify post-translational modifications 

• Capillary columns available for increased sensitivity 


Particle 

Shape/Size 

(μm) 

Pore 

Size 

(Å) 

Surface 

Area 

(m 2 /g) 

Carbon 

Load 

% 

Calculated Bonded 

Phase Coverage 

(µmole/m 2 ) 

End 

Capping 


C4 Spher. 5, 10, 15 300 170 5.0 6.30 Yes 

C5 Spher. 5, 10, 15 300 170 5.5 5.30 Yes 

C18 Spher. 3, 5, 10, 15 300 170 13.3 5.50 Yes 

Proteo Spher. 4, 10 90 475 15.0 — Yes 

Dependable Solutions for Protein/Peptide Researchers 

Jupiter HPLC columns are used throughout the life science industry in a variety of departments. 

Phenomenex offers support and solutions in all areas of protein research and manufacturing, 

especially in characterization, purification, and proteomics/biomarker discovery. 

Type of Work 

Protein Characterization 

Protein/ Peptide Purification 

Proteomics/ Biomarker Discovery 

Key Applications 

• Identify post-translational modifications 

• Analyze intact antibodies 

• Study PEGylated proteins 

• Separate target compound from impurities 

• Purify antibodies 

• Separate protein components from one another 

• Easy, direct scale up to preparative and process scales 

• Perform peptide mapping for differential proteomics 

• Identify low level proteins utilizing capillary columns 

• Excellent resin for 2nd dimension of 2D-HPLC 


Phenomenex

Jupiter ® 

HPLC 


Jupiter 300 – for Intact Protein 

Separation and Purification 

Jupiter 300 has proven its performance to biochromatographers 

worldwide as a leading 300 Å column in the market. 

• 300 Å column for analysis and purification of proteins 

> 10,000 MW 

• Highly efficient columns with long lifetime due to 

super-smooth, high-mechanical-strength 300 Å silica 

• Excellent column for LC/MS due to low phase bleed and 

exceptional performance at low buffer concentrations 

Large Proteins on Jupiter 300 C4 

Column: Jupiter 300 5 μm C4 300Å 


Part No.: 00F-4167-E0 

Mobile Phase: A: 0.1 % TFA in Water 

B: 0.08 % TFA in Acetonitrile 

Gradient: A/B (95:5) to A/B (20:80) in 20 minutes 


Temperature: 22 ˚C 


Inj. Volume: 25 μL 

Sample: 1. Bovine Serum Albumin 

2. Glutamic Dehydrogenase 

3. ß-Galactosidase 

4. Ovalbumin 

1 

3 

Selecting the Appropriate 300 Å Phase 

Jupiter 300 C4 This low hydrophobicity phase is less likely to 

cause irreversible adsorption of “sticky” proteins and allows for 

the use of shallow gradients along with lower concentrations of 

organic solvent. 

• For proteins >10,000 Da 

• For highly hydrophobic proteins 

Jupiter 300 C5 This bonded phase imparts greater pH stability 

compared to traditional C4 phase. One can expect longer column 

lifetimes and more stable, reproducible retention times because 

of the bonded phase’s increased stability to hydrolysis. 


• For highly hydrophobic proteins 

• More retentive than C4, offering slightly different selectivity 

Separation on Jupiter 300 C18 

Column: Jupiter 300 5 µm C18 300 Å 


Part No.: 00F-4053-B0 

Mobile Phase: A: 0.1 %TFA/ 95 % Water / 5 % Acetonitrile 

B: 0.085 % TFA/ 95 % Acetonitrile/ 5 % Water 

Gradient: A/B (88:12) to A/B (15:85) in 21 minutes 



mAU 

Sample: 1. Aprotinin 

800 

2. Ribonuclease 

600 

3. Lysozyme 

1 

4. Lactalbumin 

400 

5. Leptin 

200 

2 

2 

4 

3 4 

App ID 14761 

5 

App ID 14937 

Jupiter 300 C18 Excellent for polar as well as non-polar proteins; 

most retentive of Jupiter 300 phases, allowing one to separate 

proteins with slight differences in hydrophobicity 


• For hydrophilic proteins 

• Most retentive phase 

0 

0 2 4 6 8 10 12 14 16 18 min 

Sharp Peaks and High Yields 

Ultra-pure silica and dense bonded phase coverage provide sharp 

peaks for your sample by decreasing the number of non-specific 

interactions. Sharpened peak symmetry allows for easier quantitation, 

improved resolution, and separation of complex mixtures. 

High yields result in more material for downstream analysis, decreased 

number of purification runs, and lower production costs. 

Sharp Peaks for Easy Quantitation 

Column: Jupiter 300 5 µm C4 300 Å 


Part No.: 00B-4167-E0 

Mobile Phase: A: 0.1% TFA in Water 

B: 0.1% TFA in Acetonitrile 

Gradient: A/B (100:0) to A/B (80:20) in 1 min 

(20 % B/ min), then to A/B (65:35) in 

1.5 min (10 % B/ min), then to A/B 

(53.5:46.5) in 1.5 min (7.67 % B/ min), 

hold for 2 min (constant B) 



Sample: 1. Alkaline Phosphatase 

2. Cyanocobalamin 

3. RNase 

4. Insulin 

5. Transferrin 

6. Trypsin Inhibitor 

App ID 5378 

2.5 5 7.5 min 10 12.5 


49

Jupiter ® 

Jupiter Proteo – for Peptide 

Mapping and Peptide 

Purification 

Separating peptide fragments by reversed phase chromatography 

is a standard method of protein analysis and is used to 

develop well-characterized biopharmaceuticals. In an effort to 

support proteomic research and the growth of the synthetic 

peptide industry, we have engineered a column with a sorbent 

and surface chemistry that successfully leverages the physical 

and chemical properties of polypeptides. 

• Novel 90 Å, C12 bonded phase optimized for peptide 

mapping and the separation of synthetic peptides 

• Identify oxidation, deamidation, and other posttranslational 

modifications 

• Resolve peptides differing in hydrophobicity by one 

methyl group 

Identify More Peaks in Peptide Maps with Increased Peak 

Capacity 

Jupiter Proteo is designed to selectively separate and to optimize 

information on peptide fragments obtained in a protein 

digest. In the example, a Jupiter Proteo column offers better 

selectivity and resolving power compared with a commercial 

300 Å, silica-based C18 reversed phase column. 

Myoglobin Tryptic Digest 





Gradient: A/B (95:5) for 5 min, then to A/B 

(60:40) in 55 minutes 




Sample: Myoglobin Tryptic Digest 

Jupiter ® 4 μm Proteo 90 Å 

1 2 3 4 

Avg. 

118 72 71 49 

78 

78 

peaks 

Selectivity to Improve Resolution 

Jupiter Proteo can be used to resolve peptides of MW 

< 10,000 Da and often is able to separate peptides of only 

1-2 amino acid difference. By comparing the resolution of 

five peptide standards with amino acid sequences that differ 

in hydrophobicity by one methyl group each with respect to 

efficiency, selectivity, and resolution it is evident that Jupiter 

Proteo fully resolves each peptide. 

Methylene Selectivity 

HPLC 






Gradient: A/B (95:5) to 

A/B (55:45) in 20 minutes 




Sample: 1. NH2-Arg-Gly-Gly-Ala-Gly-Gly-Leu-Gly-Leu-Gly-Lys-Amide 

2. Ac-Arg-Gly-Gly-Gly-Gly-Gly-Leu-Gly-Leu-Gly-Lys-Amide 

3. Ac-Arg-Gly-Ala-Gly-Gly-Gly-Leu-Gly-Leu-Gly-Lys-Amide 

4. Ac-Arg-Gly-Val-Gly-Gly-Gly-Leu-Gly-Leu-Gly-Lys-Amide 

5. Ac-Arg-Gly-Val-Val-Gly-Gly-Leu-Gly-Leu-Gly-Lys-Amide 

Jupiter ® 4 μm Proteo 90 Å 

Agilent Technologies Zorbax ® 5 μm SB-C18 300 Å 

Alltech Associates Vydac ® 5 μm MS54 300 Å 

Alltech Associates Vydac ® 5 μm TP54 300 Å 

App ID 14429 App ID 14428 

App ID 14427 

App ID 14426 

Agilent Technologies Zorbax ® 5 μm 

SB-C18 300 Å 

1 2 3 4 

Avg. 

54 51 50 35 

48 

48 

peaks 

App ID 14411 App ID 14408 

Determining peak counts - The large number of peaks in a given tryptic digest 

makes counting peaks visually both inaccurate and subjective. For a more 

accurate approach, peak counting was performed using Agilent Technologies (HP) 

ChemStation software. Four different integration parameters at different sensitivity 

settings were used in calculating the number of peaks and an average. The 

parameters changed within each method were: minimum peak area, minimum peak 

height, peak width, and threshold. The table below describes the parameters used 

for each calculation. 

Method Threshold Peak Width Min Area Min Height 

1 1.0 0.1 10.0 20.0 

2 2.0 0.2 10.0 20.0 

3 3.0 0.3 20.0 10.0 

4 3.0 0.3 20.0 50.0 


Phenomenex

Jupiter ® 

HPLC 



4 μm & 5 μm Capillary Columns (mm) Capillary Guard Columns (mm) 

Phases 50 x 0.30 150 x 0.30 250 x 0.30 50 x 0.50 150 x 0.50 250 x 0.50 20 x 0.30 20 x 0.50 

5 μm C4 300 Å 00B-4167-AC 00F-4167-AC 00G-4167-AC 00B-4167-AF 00F-4167-AF — 03M-4167-AC 03M-4167-AF 

5 μm C18 300 Å 00B-4053-AC 00F-4053-AC 00G-4053-AC 00B-4053-AF 00F-4053-AF 00G-4053-AF 03M-4053-AC 03M-4053-AF 

4 μm Proteo 90 Å 00B-4396-AC 00F-4396-AC 00G-4396-AC 00B-4396-AF 00F-4396-AF 00G-4396-AF 03M-4396-AC — 

3 μm, 4 μm & 5 μm Microbore and Minibore Columns (mm) SecurityGuard Cartridges (mm) 

Phases 50 x 1.0 150 x 1.0 250 x 1.0 50 x 2.0 150 x 2.0 250 x 2.0 4 x 2.0* 

/10pk 

5 μm C4 300 Å 00B-4167-A0 00F-4167-A0 00G-4167-A0 00B-4167-B0 00F-4167-B0 00G-4167-B0 AJ0-4329 



4 μm Proteo 90 Å 00B-4396-A0 00F-4396-A0 00G-4396-A0 00B-4396-B0 00F-4396-B0 00G-4396-B0 AJO-6073 

— — — — /10pk 

3 μm C18 300 Å — — — 00B-4263-B0 00F-4263-B0 — AJ0-4320 

for ID: 2.0-3.0 mm 

3 μm, 4 μm & 5 μm Analytical and Preparative Columns (mm) SecurityGuard Cartridges (mm) 

Phases 50 x 4.6 150 x 4.6 250 x 4.6 250 x 10 250 x 21.2 4 x 3.0* 10 x 10 ‡ 15 x 21.2** 

/10pk /3pk /ea 

5 μm C4 300 Å 00B-4167-E0 00F-4167-E0 00G-4167-E0 00G-4167-N0 00G-4167-P0 AJ0-4330 AJ0-7225 AJ0-7231 

5 μm C5 300 Å 00B-4052-E0 00F-4052-E0 00G-4052-E0 00G-4052-N0 00G-4052-P0 AJ0-4327 AJ0-7371 — 

5 μm C18 300 Å 00B-4053-E0 00F-4053-E0 00G-4053-E0 00G-4053-N0 00G-4053-P0 AJ0-4321 AJ0-7224 AJ0-7230 

4 μm Proteo 90 Å 00B-4396-E0 00F-4396-E0 00G-4396-E0 00G-4396-N0 00G-4396-P0 AJ0-6074 AJ0-7275 AJ0-7842 

— — — /10pk — — 

3 μm C18 300 Å — 00F-4263-E0 00G-4263-E0 — — AJ0-4321 — — 

for ID: 3.2-8.0 mm 9-16 mm 18-29 mm 

10 μm Analytical and Preparative Columns (mm) SecurityGuard Cartridges (mm) 

Phases 250 x 4.6 250 x 10 250 x 21.2 250 x 30 250 x 50 4 x 3.0* 10 x 10 ‡ 15 x 21.2** 15 x 30.0 ♦ 

/10pk /3pk /ea /ea 

C4 300 Å 00G-4168-E0 00G-4168-N0 00G-4168-P0 00G-4168-U0 00G-4168-V0 AJ0-4330 AJ0-7225 AJ0-7231 AJ0-8314 

C5 300 Å 00G-4054-E0 00G-4054-N0 00G-4054-P0 — 00G-4054-V0 AJ0-4327 AJ0-7371 — — 


Proteo 90 Å 00G-4397-E0 00G-4397-N0 — — — AJ0-6074 AJ0-7275 AJ0-7842 AJ0-8304 

for ID: 3.2-8.0 mm 9-16 mm 18-29 mm 30-49 mm 

15 μm Analytical and Preparative Columns (mm) SecurityGuard Cartridges (mm) 

Phases 250 x 4.6 250 x 10 250 x 21.2 250 x 30 250 x 50 4 x 3.0* 10 x 10 ‡ 15 x 21.2** 15 x 30.0 ♦ 

/10pk /3pk /ea /ea 


C5 300 Å 00G-4056-E0 00G-4056-N0 00G-4056-P0 — 00G-4056-V0 AJ0-4327 AJ0-7371 — — 


for ID: 3.2-8.0 mm 9-16 mm 18-29 mm 30-49 mm 



‡ 

SemiPrep SecurityGuard Cartridges require holder, Part No.: AJ0-7220 

Bulk Material 

10 μm Bulk Packings 

Phase 100 g 1 kg 5 kg 10 kg 50 kg 100 kg 

Inquire Inquire Inquire Inquire Inquire Inquire 

C4 300 Å 04G-4168 04K-4168 04L-4168 04M-4168 04N-4168 04P-4168 

C5 300 Å 04G-4054 04K-4054 04L-4054 04M-4054 04N-4054 04P-4054 

C18 300 Å 04G-4055 04K-4055 04L-4055 04M-4055 04N-4055 04P-4055 

Proteo 90 Å 04G-4397 04K-4397 04L-4397 04M-4397 04N-4397 04P-4397 

**PREP SecurityGuard Cartridges require holder, Part No.: AJ0-8223 

PREP SecurityGuard Cartridges require holder, Part No.: AJ0-8277 

15 μm Bulk Packings 

Phases 100 g 1 kg 5 kg 10 kg 50 kg 100 kg 

Inquire Inquire Inquire Inquire Inquire Inquire 

C4 300 Å 04G-4169 04K-4169 04L-4169 04M-4169 04N-4169 04P-4169 

C5 300 Å 04G-4056 04K-4056 04L-4056 04M-4056 04N-4056 04P-4056 

C18 300 Å 04G-4057 04K-4057 04L-4057 04M-4057 04N-4057 04P-4057 


51

Rezex 

Sugar, Carbohydrate, and 


Carbohydrate and Organic Acid Analysis 

• Excellent resolution 

• Wide range of selectivities 

• Excellent column-to-column reproducibility 

® 

• Recommended alternative to Bio-Rad Aminex ® , Supelco ® 

Supelcogel and Waters ® Sugar-Pak at cost-effective prices 

Rezex columns contain sulfonated styrene-divinylbenzene spheres in 

4 and 8 % cross-link forms as well as various ionic forms, including 

calcium, sodium, hydrogen, potassium, lead, and silver. So all you have 

to do is select the column with the appropriate selectivity. 

Find the Column For Your Application 

Phases Available Description Applications Additional Notes 

RCM-Monosaccharide 

(L19 packing)* 

8 % cross-linked resin 

CALCIUM ionic form 

Monosaccharides and sugar alcohols from 

sweeteners and corn and cane sugars 

Di, tri, and tetra saccharides 

– Our most commonly used column type 

– Easy regeneration with calcium nitrate solutions 

RHM-Monosaccharide 


RAM-Carbohydrate 

RSO-Oligosaccharide 

RNO-Oligosaccharide 

RPM-Monosaccharide 


RNM-Carbohydrate 


ROA-Organic Acid 


RFQ-Fast Acid 

RKP-Potassium 

RCU-USP Sugar Alcohols 



HYDROGEN ionic form 


SILVER ionic form 


SILVER ionic form 


SODIUM ionic form 


LEAD ionic form 


SODIUM ionic form 






POTASSIUM ionic form 


CALCIUM ionic form 

* United States Pharmacopeia (USP) 

Monosaccharides in combination with organic acids, 

fatty acids, alcohols, ketones, neutral compounds, or 

inorganic salts 

Selectivity complimentary to other Rezex column types 

High resolution of oligosaccharides up to 18 degrees 

of polymerization (Dp) 

High resolution of oligosaccharides 

Monosaccharides and sugar alcohol analysis. 

Cellobiose, glucose, xylose, arabinose, and 

mannose & other cellulose products 

For matrices which contain high 

concentration of inorganic sodium, 

Example-molasses 

Organic acids alone or in combination 

with carbohydrates, alcohols, fatty acids, or neutral 

compounds; Amino sugars 

Rapid screening of fruit quality; ethanol, 

acetic acid, glycerol, & standard alcohol mixtures 

Analysis of glyphosate 

For sugar analysis according to the USP procedures 

– Versatile column, generally run with a 

mobile phase of deionized water 

– Guard column is recommended to 

protect the ionic integrity of the matrix 

– Easily regenerated to the original ionic strength 

– Selectivity can be altered by changing the pH as 

well as the type of dilute mineral acid used as the 

mobile phase 

– Analytes are routinely chromatographed under 

5 minutes 

– Sorbitol and mannitol can be resolved using 

simple isocratic conditions 


Phenomenex

Rezex 



Rezex vs. Bio-Rad Aminex ® 

Phenomenex guarantees satisfaction when using Rezex HPLC columns. As 

illustrated below, Rezex offers advantages that enhance chromatographic 

results, increase throughput, and simplify quantitation. 

Easier, Accurate Quantitation 

Due to improved peak shape 

Saccharides 


Column: Rezex RCM-Monosaccharide 

Aminex HPX-87C 


Mobile Phase: Water 


Detection: ELSD 


Sample: 1. Melezitose 

2. Maltose 

3. Glucose 

4. Mannose 

5. Fructose 

6. Ribitol 

Orange Juice 









Sample: 1. Sucrose 

2. Glucose 

3. Fructose 

mAU 

120 

100 

80 

60 

40 

1 2 

Superior 

Peak Shape 

3 

4 

5 

6 

Rezex 

RCM 

App ID 14975 

mAU 

500 

400 

300 

200 

100 

1 

2 

3 

Rezex 

RCM 

App ID 14981 

mAU 

100 

80 

60 

40 

0 10 20 min 

1 

2 

3 

0 10 20 min 

4 

5 

6 

Aminex 

HPX-87C 

App ID 14976 

mAU 

400 

200 

0 2 4 6 8 10 12 14 16 min 

1 

Difficult Quantitation 

2 3 

Aminex 

HPX-87C 

0 2 4 6 8 10 12 14 16 min 

App ID 14982 

Longer Column Lifetime 

Due to lower backpressures 

Aliphatic Acids 


Column: Rezex ROA-Organic Acid 

Aminex HPX-87H 


Mobile Phase: 0.005 N H 2 SO 4 




Sample: 1. Oxalic Acid 

2. Citric Acid 

3. Tartaric Acid 

4. Succinic Acid 

5. Formic Acid 

6. Acetic Acid 

Baseline Separation of Critical 

Sample Components 

Due to improved resolution 

Sugars 









Sample: 1. Sucrose 

2. Glucose 

3. Galactose 

4. Fructose 

5. Sorbitol 

mAU 

mAU 

120 

100 

80 

60 

40 

20 

0 

120 

100 

80 

60 

40 

20 

0 

Rezex 

32 

1 

0 10 20 min 

Aminex 

55 

Bar 

Bar 

1 

2 

3 

0 10 20 min 

2 

3 

4 

4 

5 

5 

6 

Rezex 

ROA 

6 

Aminex 

HPX-87H 

App ID 14963 App ID 14962 

mAU 

mAU 

80 

60 

40 

100 

80 

60 

40 

0 

1 

Baseline 

1 

Resolution 

2 

3 4 

10 20 

30 min 

2 3 4 

0 10 20 30 min 

5 

5 

Rezex 

RCM 

Aminex 

HPX-87C 

App ID 14977 

App ID 14978 


Aminex ® is a registered trademark of Bio-Rad. 

Phenomenex is in no way affilated with Bio-Rad. 


53

Rezex 



Retention Times for Some Carbohydrates and Sugar Alcohols 

Counter Ion Analyte RAM Ag + RCM Ca +2 RNM Na + RHM H + RPM Pb +2 

Adonitol (Ribitol) 11.54 14.93 11.10 11.11 20.15 

D-Altrose 11.95 12.71 11.45 10.21 15.82 

D-(-)-Arabinose 13.01 13.56 12.65 11.24 16.47 

D-(+)-Cellobiose 8.86 8.60 8.49 8.02 11.00 

D-(+)-Digitoxose 11.90 13.82 11.39 12.59 15.32 

Dulcitol 11.64 21.61 11.10 10.71 33.25 

Meso-Erythritol 12.31 15.49 11.78 12.14 19.82 

D-(-)- Fructose 12.05 13.65 11.76 10.31 17.71 

L-(-)-Fucose 12.75 13.19 12.30 11.65 16.19 

D-(+)-Galactose 11.87 11.73 11.47 10.19 14.94 

Gentiobiose 8.70 8.40 8.40 7.87 10.53 

D-(+)-Glucose 11.04 10.37 10.71 9.62 12.92 

Inositol 12.59 13.35 12.14 9.98 18.87 

Isomaltose 9.11 8.74 8.76 8.02 11.28 

Lactose 9.27 9.03 8.78 8.32 11.89 

Lactulose 9.75 10.32 9.23 8.57 13.95 

D- Lyxose 12.41 14.06 11.98 10.68 16.66 

D- Maltose 9.16 8.81 8.75 8.18 11.59 

Maltotriose 8.27 8.10 7.94 7.51 11.02 

Maltulose 9.25 9.47 8.82 8.27 12.40 

D- Mannitol 11.36 17.82 10.80 10.59 24.90 

D-(+)-Mannose 12.04 12.04 11.54 10.16 16.39 

Melibiose 9.26 9.04 8.82 8.14 11.97 

D-(+)-Melezitose 8.00 7.93 7.66 7.54* 9.94 

D-(+)-Raffinose 8.10 8.16 7.76 7.88* 10.28 

L-(+)-Rhamnose 11.50 12.18 11.00 10.90 14.47 

D-(-)-Ribose 14.59 23.38 14.34 11.42 33.48 

Salicin 18.51 18.58 17.36 14.98 26.81 

D-Sorbitol 11.91 22.45 11.39 10.83 35.97 

Stachyose 7.60 7.59 7.30 7.27 9.72 

Sucrose 9.03 8.71 8.65 9.24* 11.00 

Trehalose 8.91 8.72 8.49 8.32 11.01 

Xylitol 12.69 22.01 12.16 11.78 32.38 

D-(+)-Xylose 12.06 11.62 11.68 10.24 13.84 

* Partial hydrolysis results. 

Conditions: 


Mobile Phase: Water (degassed) 



Detection: RI @ 40 °C 

Column Cross Reference Chart 

Phenomenex ® 

Rezex Bio-Rad ® 

Aminex ® Supelco ® 

Supelcogel Waters ® 

Sugar-Pak 

RCM-Monosaccharide HPX-87C Supelcogel Ca Sugar-Pak 1 

125-0095 

RHM-Monosaccharide HPX-87H Supelcogel C-610H N/A 

125-0140 & H 

RPM-Monosaccaride HPX-87P Supelcogel Pb N/A 

125-0098 

RNM-Carbohydrate HPX-87N N/A 

N/A 

125-0143 

RSO-Oligosaccharide HPX-42A Supelcogel Ag1 N/A 

125-0097 & Ag2 

ROA-Organic Acid HPX-87H Supelcogel C-610H N/A 

125-0140 & H 

RFQ-Fast Acid Fast Acid N/A 

N/A 

125-0100 

RKP-Potassium HPX-87K Supelcogel K N/A 

125-0142 

RCU-USP Sugar Alcohols Sugar 

Alcohols 

125-0094 

N/A 

N/A 


SecurityGuard Analytical Cartridges require universal holder Part No.: KJO-4282 

Columns Guards 


Cartridges (mm) 

Description 

Part No. 

Cross 

Linkage Ionic Form Size (mm) Part No. Size (mm) 

4 x 3.0 

/10pk 

RCM-Monosaccharide 00H-0130-K0 8 % Calcium 300 x 7.8 03B-0130-K0 50 x 7.8 AJ0-4493 

RHM-Monosaccharide 00H-0132-K0 8 % Hydrogen 300 x 7.8 03B-0132-K0 50 x 7.8 AJ0-4490 

RAM-Carbohydrate 00H-0131-K0 8 % Silver 300 x 7.8 — — AJ0-4491 

RSO-Oligosaccharide 00P-0133-N0 4 % Silver 200 x 10.0 03R-0133-N0 60 x 10.0 — 

RNO-Oligosaccharide 00P-0137-N0 4 % Sodium 200 x 10.0 03R-0137-N0 60 x 10.0 — 

RPM-Monosaccharide 

(for USP procedure) 

00H-0135-K0 

00D-0135-K0 

8 % 

8 % 

Lead 

Lead 

300 x 7.8 

100 x 7.8 

03B-0135-K0 

03B-0135-K0 

50 x 7.8 

50 x 7.8 

AJ0-4492 

AJ0-4492 

RNM-Carbohydrate 00H-0136-K0 8 % Sodium 300 x 7.8 03B-0136-K0 50 x 7.8 — 

ROA-Organic Acid 00H-0138-K0 8 % Hydrogen 300 x 7.8 03B-0138-K0 50 x 7.8 AJ0-4490 

ROA-Organic Acid 00F-0138-K0 8 % Hydrogen 150 x 7.8 03B-0138-K0 50 x 7.8 AJ0-4490 

ROA-Organic Acid 00G-0138-E0 8 % Hydrogen 250 x 4.6 — — AJ0-4490 

RKP-Potassium 00H-3252-K0 8 % Potassium 300 x 7.8 — — — 

RFQ-Fast Acid 00D-0223-K0 8 % Hydrogen 100 x 7.8 03B-0223-K0 50 x 7.8 AJ0-4490 

RCU-USP Sugar Alcohols 00G-0130-D0 8 % Calcium 250 x 4.0 03A-0130-D0 30 x 4.0 AJ0-4493 

for ID: 3.2-8.0 mm 

Aminex is a registered trademark of Bio-Rad. 

Supeleogel is a trademark of Supleco. 

Sugar-Pak is a trademark of Waters Corporation. 

Rezex is a trademark of Phenomenex, Inc. 

Phenomenex is in no way affiliated with any of the companies mentioned above. 


Phenomenex

Phenogel 

GPC 


• 5 and 10 μm particle sizes 

• Narrow bore (4.6 mm ID) solvent-saver to preparative 

columns available 

• Very good alternative to Polymer Labs PLgel and 

Waters Styragel, Ultrastyragel, Styragel HT, and 

Styragel HR columns 

• Highly cross-linked for mechanical and 

chemical stability 

• Temperature stable to 140 °C 

Phenogel is available in seven different pore sizes ranging from 

50 Å to 10 6 Å Δ , and a linear bed configuration. Pore size distribution 

and pore volume are closely controlled parameters in 

the manufacturing process; accounting for the high resolution, 

tight linear calibration curves and excellent column-to-column 

reproducibility. 

Sample Elution 

Each standard dimension Phenogel column (300 x 7.8 mm) 

has an internal volume of 15 mL that is distributed as follows: 

• 3 mL is occupied by the solid portions of the gel particles 

(20 % of total column volume) 

• 6 mL is the pore volume of the packing material 


• 6 mL is the interstitial volume or volume between the gel particles 


Thus, about 6 mL of solvent must elute through each column 

before even the largest molecules can emerge, while the 

smallest molecules emerge with the total column volume of 12 

mL. This constant distribution of volume makes it possible to 

predict the amount of solvent and time necessary to complete 

any analysis. 

Technical Specifications 

Material: 

Particle Size: 

Porosities: 

Typical Pressure: 

SDVB 

5, 10 μm 

50 Å to 10 6 Å † , and mixed 

beds 

5 μm: 350 psi 

10 μm: 200 psi 

650 psi 

Maximum Pressure: 

Maximum Temperature: 140 °C 

Minimum Efficiency*: 5 μm: 45,000 p/m** 

10 μm: 35,000 p/m** 

Typical Flow Rates: 

End Fittings: 

4.6 mm ID: 0.35 mL/min 

7.8 mm ID: 1.0 mL/min 

21.2 mm ID: 8.0 mL/min 

Valco Compatible 

* Tested in THF ** For 300 x 7.8 mm ID columns 

Column Molecular Weight Calibration Curves 

Polystyrene Molecular Weight 

SEM Photos of Phenogel Polymer Beads 

Elution Volume/Column (mL) 

10 3 Å 10 6 Å 

Δ 

Pore Size is expressed in Angstroms (10-10 meters). This is actually a 

convention used by manufacturers to indicate the approximate molecular 

weight of compounds that can be separated on a given SEC packing; 

these values do not indicate the actual size (diameter) of the pores on 

the surface of the particle. Exclusion Limit is expressed in daltons (the 

molecular weight) of the specified compound excluded from the pores 

of the base material. Practically speaking however, the exclusion limit is 

more accurately a reflection of the hydrodynamic volume occupied by the 

solvated compound. 

Column Selection by Molecular Weight 

Sample Type 

Molecular 

Weight 

Phenogel 

Column 

Small Organics 100 - 3 K 

500 - 6 K 

1 K - 15 K 

50 Å 

100 Å 

500 Å 

Resins 

1 K - 75 K 

5 K - 500 K 

10 K - 1,000 K 

10 3 Å 

10 4 Å 

10 5 Å 

High MW Polymers 60 K - 10,000 

K 

100 - 10,000 K 

10 6 Å 

Linear(2) 


55

Phenogel 

GPC/SEC Columns 

GPC 



5 μm Columns (mm) Guards 

300 x 7.8 600 x 7.8 300 x 21.2 50 x 7.8 

Pore Size MW Range 

50 Å 100-3 K 00H-0441-K0 — — 03B-2088-K0 

100 Å 500-6 K 00H-0442-K0 00K-0442-K0 — 03B-2088-K0 

500 Å 1K-15 K 00H-0443-K0 — — 03B-2088-K0 

10 3 Å 1K-75 K 00H-0444-K0 — — 03B-2088-K0 

10 4 Å 5K-500 K 00H-0445-K0 00K-0445-K0 00H-0445-P0 03B-2088-K0 

10 5 Å 10K-1,000 K 00H-0446-K0 00K-0446-K0 00H-0446-P0 03B-2088-K0 

10 6 Å 60K-10,000 K 00H-0447-K0 — 00H-0447-P0 03B-2088-K0 

300 x 7.8 600 x 7.8 — 50 x 7.8 

Mixed Beds — 

Linear(2) 100-10,000 K 00H-3259-K0 00K-3259-K0 — 03B-2088-K0 

Other Shipping Solvents: 

Methanol, Methylene Chloride, Cyclohexane, Ethyl 

Acetate, NMP, DMAC, DMF 

Size (mm) 

Price 

30 x 4.6 

$ 75 

50 x 4.6 

75 

300 x 4.6 

125 

300 x 7.8 

150 

600 x 7.8 

200 

300 x 21.2 

250 

600 x 21.2 

470 

NOTE: Phenogel columns are routinely shipped in THF. 

Columns can be shipped in Toluene and Chloroform upon 

request at no additional charge. 

5 μm Narrow Bore (NB) Columns (mm) Guards 

300 x 4.6 30 x 4.6 

Pore Size 

MW Range 

50 Å 100-3 K 00H-0441-E0 03A-2088-E0 

100 Å 500-6 K 00H-0442-E0 03A-2088-E0 

500 Å 1K-15 K 00H-0443-E0 03A-2088-E0 

10 3 Å 1K-75 K 00H-0444-E0 03A-2088-E0 

10 4 Å 5K-500 K 00H-0445-E0 03A-2088-E0 

Phenogel Columns are a 

Recommended Alternative to: 

Manufacturer Columns 

Jordi Associates Jordi GPC-DVB 

Polymer Labs 

PLgel 

Waters Styragel ® 

µStyragel 

Ultrastyragel 

Styragel ® HT 

Styragel ® HR 

10 μm Columns (mm) Guards 

300 x 7.8 600 x 7.8 300 x 21.2 600 x 21.2 50 x 7.8 

Pore Size MW Range 

50 Å 100-3 K 00H-0641-K0 00K-0641-K0 00H-0641-P0 00K-0641-P0 03B-2090-K0 

100 Å 500-6 K 00H-0642-K0 00K-0642-K0 00H-0642-P0 00K-0642-P0 03B-2090-K0 

500 Å 1K-15 K 00H-0643-K0 00K-0643-K0 — 00K-0643-P0 03B-2090-K0 

10 3 Å 1K-75 K 00H-0644-K0 00K-0644-K0 00H-0644-P0 00K-0644-P0 03B-2090-K0 

10 4 Å 5K-500 K 00H-0645-K0 00K-0645-K0 00H-0645-P0 00K-0645-P0 03B-2090-K0 

10 5 Å 10K-1,000 K 00H-0646-K0 00K-0646-K0 00H-0646-P0 00K-0646-P0 03B-2090-K0 

10 6 Å 60K-10,000 K 00H-0647-K0 00K-0647-K0 00H-0647-P0 00K-0647-P0 03B-2090-K0 

300 x 7.8 600 x 7.8 300 x 21.2 — 50 x 7.8 

Mixed Beds — 

Linear(2) 100-10,000 K 00H-3260-K0 00K-3260-K0 00H-3260-P0 — 03B-2090-K0 

All other column dimensions available. Phenogel 

columns are routinely shipped in THF. However, 

columns are also available in commonly used 

solvents such as Toluene and Chloroform as 

well as DMF, NMP, and other solvents. Refer 

to the chart above for the additional charge for 

these shipping solvents. Please specify shipping 

solvent when ordering. 


Phenomenex

Axia Packed Preparative HPLC 


U.S. Patent No. 7, 674, 383 

Preparative Chromatography Redefined 

Axia patented technology is an advanced column packing and 

hardware design that eliminates media bed collapse as a source 

of premature failure in chiral and achiral preparative columns. 

2006 R&D 100 


Compare Lifetime 

Axia Packed 

Luna ® 5 µm C18(2) Axia Packed 

Leading Competitive Preparative Column 

Waters ® XTerra ® 5 µm Prep MS C18 OBD 

mAu mAu 

1 1 

2 2 

3 3 

mAu mAu 

1 1 

2 2 

3 3 

500 500 

vs. 

500 500 

0 0 

App ID 15972 

0 0 

App ID 15933 

2 2 

3 3 

4 4 min min 

2 2 3 3 

4 4 min min 

First Run 800th Run First Run 120th Run † 

† Six columns tested. Best lifetime chosen for comparison. 

Conditions for all except where noted: 

Columns: Luna ® 5 µm C18(2) Axia Packed 

Waters ® XTerra ® 5 µm Prep MS C18 OBD 

Dimension: 50 x 21.2 mm (Luna ® ) 

50 x 19 mm (XTerra ® ) 

Mobile Phase: A: 0.5 % TFA in Water B: 0.5 % TFA in Acetonitrile 

Gradient: Linear 95:5 (A/B) to 5:95 (A/B) over 7 min, hold 3 min 

Injection Volume: 500 μL 

Flow Rate: 30 mL/min (Luna ® ) 

24 mL/min (XTerra ® ) ‡ 



Sample: 1. Triprolidine 1.6 mg 

2. Methacycline 16 mg 

3. Amitriptyline 5.25 mg 

‡ 

Same linear velocity 


57



U.S. Patent No. 7, 674, 383 

Selectivity Options 

Stationary Phase Selectivity 

With high surface areas, Phenomenex media—Gemini ® -NX and Gemini (375 m 2 /g), Luna ® (400 m 2 /g) and Synergi (475 m 2 /g) — 

maximize loading capabilities. Use the selection tree below to select the best media for your targeted purification. 

Column Selection Tree 

Achiral 

Chiral 

Yes 

Aromatic? 

No 

Yes 

Basic Compounds? No 

Yes 

Aromatic? 

No 





Lux Amylose-2 

Gemini C6-Phenyl 

(Basic mobile phase pH 

recommended) 

Gemini-NX C18 

Gemini C18 

(Basic mobile phase 

pH recommended) 

Gemini, Lux and Luna are registered trademarks of Phenomenex, Inc. 

Synergi is a trademark of Phenomenex, Inc. 


Luna Phenyl-Hexyl 

Yes 


Synergi Hydro-RP 

Polar? 

No 

Gemini-NX C18 

Luna C8(2) or C18(2) 

Synergi Max-RP 

pH-LC 

In reversed phase chromatography, compounds retain better when neutral. With the advent of pH stable (1-12) media such as 

Gemini-NX C18, Gemini C18 and C6-Phenyl, improving retention and resolution of basic compounds at high pH is now possible 

without compromising column lifetime. Under these conditions you can easily double or triple the loading compared to your current 

low pH purifications. 

pH Selectivity 

Increase Sample Load 

20000 

10000 

0 

0 

1 

Low pH 

Column: Luna ® 5 µm C18(2) 

Axia Packed 


Part No.: 00B-4252-P0-AX 


B: 0.5 % TFA in 

Acetonitrile 

Gradient: Linear 95:5 (A/B) to 

5:95 (A/B) 

over 7 min, hold 3 min 







3 


2.5 5 7.5 min 

20000 

10000 

High pH 

Columns: Gemini ® 10 µm C18 

Axia Packed 



Mobile Phase: A: 95:5 50 mM 

Ammonium Bicarbonate, 

pH 10.0 / Acetonitrile 

B: 5:95 50 mM 

Ammonium Bicarbonate, 

pH 10.0 / Acetonitrile 

Gradient: 7 min 100 % A to 

20000 

2 

100 % B over 

7 min, equilibrate 

10000 

for 3 min 

0 






0 

0 

2 

1 

Luna ® 5 μm C18(2) 

Axia Packed 

2 

3 

2.5 5 7.5 min 

Gemini ® 10 μm C18 

Axia Packed 

0 5 

2.5 7.5 min 

1 

3 

App ID 15973 App ID 15928 

30 mg load 

2500 

2000 

1500 

1000 

2500 

2000 0 

1500 -500 

2500 

20000 

1000 

10000 

2000 

500 

1500 

0 

3000 

1000 

-500 

2500 

500 

2000 

3000 

500 

2500 

0 

2000 

-500 

1500 3000 

1000 2500 

2000 500 

15000 

3000 

1000 -500 

2500 

3000 

500 

1000 2500 

2000 500 

15000 

0 

0 

0 

2 

2 

0 5 

2.5 7.5 min 

1 

0 

1500 

30 -500 mg load 

1000 0 

1 

2 

500 

2000 

0 

1500 

-500 

1000 

1 

0 1 

2 

2500 

0 

2000 

60 mg load 

0 1 

2 

1500 3000 

1000 

500 

0 

1 

0 1 

2 

0 1 

2 

0 1 

0 1 

2 

2 

2 

2 

1 

1 

1 

2 

2 

3 4 5 min 

Increase pH for basic compounds 

1 

3 

3 4 5 min 

2 

3 4 5 min 

1 

3 4 5 min 

1 

1 

2 

3 4 5 min 

2 

Baseline Resolution 

for Higher Purity 

1 

2 

3 4 5 min 

3 4 

2 

5 min 

1 

2 

pH 2.0 

pH 10.0 

App ID 17727 App ID 17726 

App ID 17725 

3 4 5 min 

3 4 5 min 

Low pH 

Column: Luna ® 5 µm C18(2) 

Axia Packed 



Mobile Phase: A: 0.5 % TFA in 

Water, pH 2.0 


Gradient: 95:5 to 5:95 (A/B) 

over 4 minutes 




Sample: 1. Propranolol 


High pH 

Column: Gemini ® 5µm C18 

Axia Packed 



Mobile Phase: A: 50 mM 

Ammonium 

Bicarbonate, pH 10.0 


Gradient: 95:5 to 5:95 (A/B) 

over 5 minutes 




Sample: 1. Propranolol 



Phenomenex



U.S. Patent No. 7, 674, 383 

Axia Packed Columns 


Phases 

Part No. 

50 x 21.2 mm 

Gemini 5 µm C18 

00B-4435-P0-AX 


00B-4436-P0-AX 

Gemini-NX 5 µm C18 

00B-4454-P0-AX 


00B-4455-P0-AX 

Luna 5 µm C18(2) 

00B-4252-P0-AX 

Luna 10 µm C18(2) 

00B-4253-P0-AX 


00B-4249-P0-AX 


00B-4250-P0-AX 

Luna 5 µm CN 

00B-4255-P0-AX 

Luna 5 µm PFP(2) 

00B-4448-P0-AX 

Luna 5 µm Phenyl-Hexyl 00B-4257-P0-AX 

Luna 5 µm HILIC 

00B-4450-P0-AX 

Luna 5 µm Silica(2) 

00B-4274-P0-AX 

Synergi 4 µm Fusion-RP 00B-4424-P0-AX 

Synergi 4 µm Max-RP 

00B-4337-P0-AX 

Synergi 4 µm Hydro-RP 00B-4375-P0-AX 

Synergi 4 µm Polar-RP 00B-4336-P0-AX 

75 x 21.2 mm 


00C-4435-P0-AX 


00C-4454-P0-AX 


00C-4252-P0-AX 

100 x 21.2 mm 


00D-4435-P0-AX 


00D-4436-P0-AX 

Gemini 5 µm C6-Phenyl 00D-4444-P0-AX 


00D-4454-P0-AX 

Gemini-NX 10 µm C18 00D-4455-P0-AX 

Jupiter 10 µm C18 

00D-4455-P0-AX 


00D-4168-P0-AX 

Jupiter 10 µm Proteo 

00D-4397-P0-AX 


00D-4252-P0-AX 

Luna 10 µm C18(2) 

00D-4253-P0-AX 


00D-4249-P0-AX 

Luna 10 µm C5 

00D-4092-P0-AX 


00D-4448-P0-AX 

Luna 5 µm Phenyl-Hexyl 00D-4257-P0-AX 

Luna 5 µm NH 2 

00D-4378-P0-AX 


00D-4450-P0-AX 

Luna 5 µm Silica (2) 

00D-4274-P0-AX 


00D-4472-P0-AX 


00D-4459-P0-AX 


00D-4457-P0-AX 

Synergi 4 µm Fusion-RP 00D-4424-P0-AX 


00D-4337-P0-AX 

Synergi 10 µm Max-RP 00D-4350-P0-AX 

Synergi 4 µm Hydro-RP 00D-4375-P0-AX 

Synergi 4 µm Polar-RP 00D-4336-P0-AX 

150 x 21.2 mm 


00F-4435-P0-AX 


00F-4436-P0-AX 

Gemini 5 µm C6-Phenyl 00F-4444-P0-AX 


00F-4454-P0-AX 


00F-4455-P0-AX 


00F-4252-P0-AX 

Luna 10 µm C18(2) 

00F-4253-P0-AX 


00F-4249-P0-AX 


00F-4250-P0-AX 

Luna 5 µm CN 

00F-4255-P0-AX 


00F-4378-P0-AX 


00F-4448-P0-AX 

Luna 5 µm Phenyl-Hexyl 00F-4257-P0-AX 


00F-4450-P0-AX 


00F-4274-P0-AX 

Phases 

150 x 21.2 mm (cont’d) 





Synergi 4 µm Hydro-RP 

Synergi 4 µm Polar-RP 

Synergi 4 µm Fusion-RP 

250 x 21.2 mm 



Gemini 5 µm C6-Phenyl 







Luna 10 µm C18(2) 




Luna 5 µm CN 



Luna 5 µm Phenyl-Hexyl 












SecurityGuard PREP System 

(Highly recommended for extending column lifetime) 

Protect your Axia Packed column and prolong its lifetime with Security- 

Guard , the advanced HPLC guard cartridge system. 

• Get full protection with minimal impact on your chromatographic results. 

• Contaminants are retained by an inexpensive, 15 x 21.2 or 15 x 30 mm ID 

disposable cartridge 


Part No. 

00F-4472-P0-AX 

00F-4459-P0-AX 

00F-4457-P0-AX 

00F-4337-P0-AX 

00F-4375-P0-AX 

00F-4336-P0-AX 

00F-4424-P0-AX 

00G-4435-P0-AX 

00G-4436-P0-AX 

00G-4444-P0-AX 

00G-4454-P0-AX 

00G-4455-P0-AX 

00G-4168-P0-AX 

00G-4396-P0-AX 

00G-4397-P0-AX 

00G-4252-P0-AX 

00G-4253-P0-AX 

00G-4249-P0-AX 

00G-4250-P0-AX 

00G-4092-P0-AX 

00G-4255-P0-AX 

00G-4378-P0-AX 

00G-4448-P0-AX 

00G-4257-P0-AX 

00G-4274-P0-AX 

00G-4091-P0-AX 

00G-4472-P0-AX 

00G-4459-P0-AX 

00G-4457-P0-AX 

00G-4493-P0-AX 

00G-4491-P0-AX 

00G-4337-P0-AX 

00G-4375-P0-AX 

00G-4336-P0-AX 

00G-4424-P0-AX 

SecurityGuard PREP System 

Part No. Description Unit 

AJ0-8223 SecurityGuard PREP HPLC Guard Cartridge ea 

Holder Kit, 21.2 mm ID, includes column coupler 

AJ0-8277 SecurityGuard PREP HPLC Guard Cartridge 

Holder Kit, 30.0 mm ID, includes column coupler 

ea 

For additional sizes not displayed, please visit the individual 

product pages or contact your Phenomenex technical 

consultant or local distributor. 


59

Axia 

Packed Preparative HPLC 

U.S. Patent No. 7, 674, 383 


Ordering Information (cont’d) 

Phases 

50 x 30 mm 






Luna 10 µm C18(2) 












75 x 30 mm 





Luna 10 µm C18(2) 






100 x 30 mm 









Luna 10 µm C18(2) 


Luna 5 µm CN 















Part No. 

00B-4435-U0-AX 

00B-4436-U0-AX 

00B-4454-U0-AX 

00B-4455-U0-AX 

00B-4252-U0-AX 

00B-4253-U0-AX 

00B-4249-U0-AX 

00B-4250-U0-AX 

00B-4257-U0-AX 

00B-4448-U0-AX 

00B-4274-U0-AX 

00B-4337-U0-AX 

00B-4350-U0-AX 

00B-4375-U0-AX 

00B-4376-U0-AX 

00B-4336-U0-AX 

00B-4351-U0-AX 

00C-4435-U0-AX 

00C-4444-U0-AX 

00C-4454-U0-AX 

00C-4252-U0-AX 

00C-4253-U0-AX 

00C-4249-U0-AX 

00C-4257-U0-AX 

00C-4337-U0-AX 

00C-4375-U0-AX 

00C-4336-U0-AX 

00D-4435-U0-AX 

00D-4436-U0-AX 

00D-4444-U0-AX 

00D-4454-U0-AX 

00D-4455-U0-AX 

00D-4055-U0-AX 

00D-4397-U0-AX 

00D-4252-U0-AX 

00D-4253-U0-AX 

00D-4249-U0-AX 

00D-4255-U0-AX 

00D-4378-U0-AX 

00D-4448-U0-AX 

00D-4257-U0-AX 

00D-4274-U0-AX 

00D-4472-U0-AX 

00D-4459-U0-AX 

00D-4457-U0-AX 

00D-4337-U0-AX 

00D-4350-U0-AX 

00D-4375-U0-AX 

00D-4336-U0-AX 

00D-4351-U0-AX 

00D-4424-U0-AX 

00D-4425-U0-AX 

For additional sizes not displayed, please visit the individual 

product pages or contact your Phenomenex technical 

consultant or local distributor. 

Phases 

150 x 30 mm 






Luna 10 µm C18(2) 







250 x 30 mm 










Luna 10 µm C18(2) 



Luna 5 µm CN 









50 x 50 mm 






Luna 10 µm C18(2) 


100 x 50 mm 



Luna 10 µm C18(2) 

150 x 50 mm 




250 x 50 mm 



Luna 10 µm C18(2) 

Luna 15 µm C18(2) 








Part No. 

00F-4435-U0-AX 

00F-4436-U0-AX 

00F-4454-U0-AX 

00F-4455-U0-AX 

00F-4252-U0-AX 

00F-4253-U0-AX 

00F-4378-U0-AX 

00F-4448-U0-AX 

00F-4472-U0-AX 

00F-4459-U0-AX 

00F-4457-U0-AX 

00F-4336-U0-AX 

00G-4435-U0-AX 

00G-4436-U0-AX 

00G-4454-U0-AX 

00G-4455-U0-AX 

00G-4055-U0-AX 

00G-4168-U0-AX 

00G-4396-U0-AX 

00G-4397-U0-AX 

00G-4252-U0-AX 

00G-4253-U0-AX 

00G-4249-U0-AX 

00G-4092-U0-AX 

00G-4255-U0-AX 

00G-4378-U0-AX 

00G-4448-U0-AX 

00G-4450-U0-AX 

00G-4274-U0-AX 

00G-4472-U0-AX 

00G-4459-U0-AX 

00G-4457-U0-AX 

00G-4336-U0-AX 

00B-4435-V0-AX 

00B-4436-V0-AX 

00B-4454-V0-AX 

00B-4455-V0-AX 

00B-4252-V0-AX 

00B-4253-V0-AX 

00B-4285-V0-AX 

00D-4436-V0-AX 

00D-4455-V0-AX 

00D-4253-V0-AX 

00F-4436-V0-AX 

00F-4455-V0-AX 

00F-4448-V0-AX 

00G-4436-V0-AX 

00G-4455-V0-AX 

00G-4253-V0-AX 

00G-4273-V0-AX 

00G-4250-V0-AX 

00G-4272-V0-AX 

00G-4092-V0-AX 

00G-4450-V0-AX 

00G-4472-V0-AX 

00G-4459-V0-AX 

00G-4457-V0-AX 


Phenomenex

2011 Key Products Guide - Fortune Scientific Co., Ltd.

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