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O - Swisstransfusion

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• Plasma or serum samples of all 6,387 patients tested in our<br />

laboratory from January 1 thru December 31, 2007 stored at -30°C.<br />

• Look back procedure (January 2008) identifying patients who<br />

randomly received ABO variants with non-deletional O alleles<br />

• In patients with follow up samples Titration of anti-A and anti-B<br />

in samples at the time of the cross-match of the ABO variant<br />

and in follow up samples in gel technique using A1 and B red cells:<br />

• Neutral gel cards: incubation 15 minutes at room temperature<br />

• Antiglobulin gel cards: incubation 15 minutes at 37°C

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