O - Swisstransfusion
O - Swisstransfusion O - Swisstransfusion
• Routine ABO, (RhD, RhCcEe, K) phenotyping of first and second time donors on Olympus PK 7200 with monoclonal anti-A, anti-B reagents and reverse typing (A1, A2, B, O red cells) • Unclear results in ABO proved in tube test (incubation 30 minutes at room temperature) with monoclonal anti-A, anti-B reagents and reverse typing (A1, A2, B, O red cells) Since May 2004 • Samples with weak or negative A and B antigens and missing or weak (+) corresponding isoagglutinins Low resolution ABO genotyping for A, A2, B, O01, O03 alleles (PCR-SSP) • If no correlation between phenotype and genotype ABO subtyping for 36 rare alleles (Ael, Ax, Aw, Bx, Bw, etc.) (PCR-SSP) • If still no correlation between phenotype and genotype Exon amplification and sequencing of entire ABO gene from genomic DNA
Results Donors with aberrant ABO phenotypes • May 2004 thru December 2007: 206,262 first and second time donors • 412 samples with aberrant ABO phenotypes (0.20%) 45 samples with weak antigens Ax01 (646T>A): n=15 Aw06 (502C>G): n=5 Aw13 (2T>C): n=3 Ael01 (804insG): n=2 Ax03, Aw04, Aw09, B(A)03: n=4 (one each) novel variant (Bw21): n=1 347 samples with negative antigens and weak or absent isoagglutinins non-deletional O alleles (O03, Aw08, O51): n=255 other alleles (Ax03, Ax01): n=6 novel variants (Bw20, Aw15): n=3 molecular basis unknown: 123 molecular basis unknown: n=15
- Page 1 and 2: ABO-Blutgruppensystem Swisstransfus
- Page 3: Genetik der ABO-Blutgruppen Chromos
- Page 6 and 7: Serumeigenschaften : Anti-A1: ++ An
- Page 8: Anti-A1: + Anti-A2: neg Anti-B: +++
- Page 12: Ax03/O03 Ax01/O01
- Page 18 and 19: Clinical significance of non-deleti
- Page 20 and 21: • 20,566 red cell units cross-mat
- Page 22 and 23: Anti-A Titer before and after Trans
- Page 24 and 25: Anti-A Titer before and after Trans
- Page 26: Conclusions: • The increasing num
- Page 29 and 30: Intron 1 (reverse) IVS1+2insT wild
- Page 31 and 32: Exon 7 wild type: mutant: 815G>A T
- Page 33: Genetische Vielfalt im ABO System
- Page 43 and 44: Acknowledgments Peter Bugert Gaby R
- Page 46: Schwaches A-Antigen
- Page 50 and 51: A-Antigen-Verlust (MDS-Patient)
Results<br />
Donors with aberrant ABO phenotypes<br />
• May 2004 thru December 2007: 206,262 first and second time donors<br />
• 412 samples with aberrant ABO phenotypes (0.20%)<br />
45 samples with weak antigens<br />
Ax01 (646T>A): n=15<br />
Aw06 (502C>G): n=5<br />
Aw13 (2T>C): n=3<br />
Ael01 (804insG): n=2<br />
Ax03, Aw04, Aw09,<br />
B(A)03: n=4 (one each)<br />
novel variant (Bw21): n=1<br />
347 samples with negative antigens<br />
and weak or absent isoagglutinins<br />
non-deletional O alleles<br />
(O03, Aw08, O51): n=255<br />
other alleles (Ax03, Ax01): n=6<br />
novel variants (Bw20,<br />
Aw15): n=3<br />
molecular basis unknown: 123<br />
molecular basis unknown: n=15
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