O - Swisstransfusion
O - Swisstransfusion
O - Swisstransfusion
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• Routine ABO, (RhD, RhCcEe, K) phenotyping of first and second time<br />
donors on Olympus PK 7200 with monoclonal anti-A, anti-B reagents<br />
and reverse typing (A1, A2, B, O red cells)<br />
• Unclear results in ABO proved in tube test (incubation 30 minutes<br />
at room temperature) with monoclonal anti-A, anti-B reagents and<br />
reverse typing (A1, A2, B, O red cells)<br />
Since May 2004<br />
• Samples with weak or negative A and B antigens and missing or<br />
weak (+) corresponding isoagglutinins<br />
Low resolution ABO genotyping for A, A2, B, O01, O03 alleles (PCR-SSP)<br />
• If no correlation between phenotype and genotype<br />
ABO subtyping for 36 rare alleles (Ael, Ax, Aw, Bx, Bw, etc.) (PCR-SSP)<br />
• If still no correlation between phenotype and genotype<br />
Exon amplification and sequencing of entire ABO gene from genomic DNA
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