Cell-Based Assays for Hepatotoxicity:
Cell-Based Assays for Hepatotoxicity: Cell-Based Assays for Hepatotoxicity:
Preliminary data: comparison of HCA methods with biochemical assays for toxicity Nocodazole and K-252a: Toxicity detected by HCA assay (neurite outgrowth parameter) Toxicity effect NOT detected with MTT or LDH assay
Summary Cell-based assays for hepatotoxicity and neurotoxicity • Assays for hepatotoxicity using HCA have been adopted for HepG2 cells. Early indications are that the assays can be used as a “filter” to detect toxic effects earlier in drug discovery process • Assays for neurotoxicity using HCA appear to show better sensitivity than traditional biochemistry assays for cytotoxicity. More data required to understand various mechanisms of action and also develop predictive indices HCA assays for toxicology may identify potential toxicity before expensive preclinical animal testing. These assays will also enable prioritization of lead compounds during screening.
- Page 1 and 2: Cell-Based Assays for Hepatotoxicit
- Page 3 and 4: Now: High Content Analysis Automate
- Page 5 and 6: Inability to accurately screen for
- Page 7 and 8: Development of a high content analy
- Page 9 and 10: Development of a high content analy
- Page 11 and 12: HepG2 cells treated with Campotheci
- Page 13 and 14: Development of high content analysi
- Page 15 and 16: Cellular Targets for Neurotoxicity
- Page 17 and 18: Assays for detecting toxicity in as
- Page 19 and 20: Detecting changes in neuronal and a
- Page 21 and 22: Assessment of toxin effects on syna
- Page 23 and 24: Preliminary data: comparison of HCA
- Page 25: Preliminary data: comparison of HCA
Preliminary data: comparison of HCA methods with biochemical<br />
assays <strong>for</strong> toxicity<br />
Nocodazole and K-252a:<br />
Toxicity detected by HCA assay (neurite outgrowth parameter)<br />
Toxicity effect NOT detected with MTT or LDH assay
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