Cell-Based Assays for Hepatotoxicity:
Cell-Based Assays for Hepatotoxicity: Cell-Based Assays for Hepatotoxicity:
Development of HCA assays for neurotoxicity • Assays for neurite outgrowth in neurons • Assays for detecting toxicity in astrocytes • Assays for detecting synaptogenesis in neurons • Assays for detecting toxicity in co-cultures of astrocytes and neurons
Assays for detecting toxicity in astrocytes Primary rat hippocampal astrocytes Cells treated with two different toxins, paclitaxel and nocodazole Both toxins cause increased expression of GFAP as the levels of toxin rise. Different toxins cause different effects on the expression of GFAP 0.4% DMSO CONTROL (4hr) 1µM Paclitaxel TOXIN (4hr)
- Page 1 and 2: Cell-Based Assays for Hepatotoxicit
- Page 3 and 4: Now: High Content Analysis Automate
- Page 5 and 6: Inability to accurately screen for
- Page 7 and 8: Development of a high content analy
- Page 9 and 10: Development of a high content analy
- Page 11 and 12: HepG2 cells treated with Campotheci
- Page 13 and 14: Development of high content analysi
- Page 15: Cellular Targets for Neurotoxicity
- Page 19 and 20: Detecting changes in neuronal and a
- Page 21 and 22: Assessment of toxin effects on syna
- Page 23 and 24: Preliminary data: comparison of HCA
- Page 25 and 26: Preliminary data: comparison of HCA
- Page 27 and 28: Summary Cell-based assays for hepat
Development of HCA assays <strong>for</strong> neurotoxicity<br />
• <strong>Assays</strong> <strong>for</strong> neurite<br />
outgrowth in neurons<br />
• <strong>Assays</strong> <strong>for</strong> detecting<br />
toxicity in astrocytes<br />
• <strong>Assays</strong> <strong>for</strong> detecting<br />
synaptogenesis in<br />
neurons<br />
• <strong>Assays</strong> <strong>for</strong> detecting<br />
toxicity in co-cultures of<br />
astrocytes and neurons
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