2D NMR Spectrum Processing with Mnova - UCSB Chem and ...
2D NMR Spectrum Processing with Mnova - UCSB Chem and ... 2D NMR Spectrum Processing with Mnova - UCSB Chem and ...
• For small molecules, NOEs mostly have opposite signs with the diagonal peaks. • Typical artifacts are: • dispersive peaks (with mixed up/down signals) that come from through-bond COSY-correlations. These are suppressed but not completely. • exchange crosspeaks having same sign with diagonal peaks Incomplete suppression of COSY-type peaks (peaks have both positive and negative signals)
These through-bond COSY-type peaks tend to cancel some or all through-space NOEs because they have opposite signs in some overlapping areas. Here, more COSY-type peaks are seen between the aromatic ring protons.
- Page 1 and 2: 2D NMR Spectrum Processing with Mno
- Page 3 and 4: 2D NMR Spectrum Processing with Mno
- Page 5 and 6: 2D Processing Adjustment in Mnova S
- Page 7 and 8: gCOSY: Initial look after automatic
- Page 9 and 10: Apply baseline correction along f1
- Page 11 and 12: Turn on Processing→Reduce t1 nois
- Page 13 and 14: Select View→2D Plotting Method→
- Page 15 and 16: Referencing Set crosshair over the
- Page 17 and 18: The 2D peaks will look smoother and
- Page 19 and 20: After PH0 adjustment along f2
- Page 21 and 22: • For f1, select a peak near the
- Page 23 and 24: After f2 baseline correction
- Page 25: Additional processing • Processin
• For small molecules, NOEs mostly have opposite signs <strong>with</strong> the diagonal peaks.<br />
• Typical artifacts are:<br />
• dispersive peaks (<strong>with</strong> mixed up/down signals) that come from through-bond<br />
COSY-correlations. These are suppressed but not completely.<br />
• exchange crosspeaks having same sign <strong>with</strong> diagonal peaks<br />
Incomplete suppression of COSY-type peaks<br />
(peaks have both positive <strong>and</strong> negative signals)
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