Relative Quantification - CRN2M
Relative Quantification - CRN2M
Relative Quantification - CRN2M
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Biological Variation<br />
<br />
What factors may have an impact on the situation found in<br />
the sample<br />
– Gender<br />
– Age of the individual<br />
– Whether sample was taken during the day or in the night<br />
– Single cell type or mixture of cells (whole tissue)<br />
– ...<br />
– Biological variation within a „normal“ or<br />
affected/disease/treated group<br />
17<br />
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During the Study<br />
<br />
Load equal amount of RNA (converted to cDNA) into all<br />
wells<br />
– Endogenous control should be almost equally expressed in<br />
all samples<br />
– All samples will have almost identical Ct values for the<br />
endogenous control = quality control<br />
18<br />
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