Relative Quantification - CRN2M
Relative Quantification - CRN2M
Relative Quantification - CRN2M
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Exercise 2<br />
Brain<br />
(Calibrator)<br />
Liver<br />
18S rRNA Ct 14<br />
IL-10 Ct 32<br />
18S rRNA Ct 16<br />
IL-10 Ct 28<br />
How does the expression of IL-10 differ<br />
in Liver and Brain<br />
∆Ct Brain = 18<br />
∆Ct Liver = 12 12 -18 = -6 2 6 = 64<br />
IL-10 is 64-times higher expressed in Liver!<br />
30<br />
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Exercises: <strong>Relative</strong> <strong>Quantification</strong><br />
ABI PRISM ® 7300/7500 SDS<br />
System
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