Relative Quantification - CRN2M
Relative Quantification - CRN2M
Relative Quantification - CRN2M
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Calibrator Concept: an Example Using four<br />
Samples<br />
<br />
<br />
<br />
The calibrator is one of the sample of the study.<br />
The calibrator is usually the normal physiological state of<br />
the study : i.e<br />
– Untreated cells<br />
– Normal glycemia<br />
– Non tumoral tissue<br />
If working on animals, multiple samples can be<br />
considered as calibrator<br />
21<br />
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Calibrator Concept: an Example Using four Samples<br />
Calibrator<br />
t=0 t=12 t=24 t=48<br />
time<br />
total RNA<br />
total RNA<br />
total RNA<br />
total RNA<br />
cDNA<br />
cDNA<br />
cDNA<br />
cDNA<br />
22<br />
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