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FUNGI AND LICHENS IN THE BALTICS AND BEYOND XVIII ...

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1. Disc surface waxy-looking – Belonia herculina, B. russula, Coenogonium luteum,<br />

C. pineti, Gyalecta foveolaris, G. geoica, G. liguriensis, G. peziza, G. schisticola, G. titovii,<br />

Pachyphiale carneola, P. fagicola, P. ophiospora.<br />

2. Disc surface without waxy cover. Paraphyse tips of different shapes are visible on<br />

the apothecia surface: 2.1. Capitate – Gyalecta flotovii, G. friesii, G. kukriensis, G. subclausa,<br />

G. truncigena, G. ulmi; 2.2. Reticulate – Gyalecta nigricans; 2.3. Crater-like – Gyalecta<br />

jenensis; 2.4. Linguiform – Gyalecta derivate.<br />

3. Disc surface with numerous crystals – Gyalecta leucaspis.<br />

<strong>THE</strong> PRELIM<strong>IN</strong>ARY RESULTS ON ANTIBACTERIAL ACTIVITY OF EXTRACTS<br />

FROM <strong>IN</strong> VITRO CULTURED MYCELIA OF Protoparmeliopsis muralis<br />

B. GUZOW-KRZEMIŃSKA 1 , J. DYBUŚĆ 2<br />

Department of Molecular Biology, University of Gdańsk, Kładki 24, 80-822 Gdańsk, Poland<br />

1 E-mail: beatagk@biotech.ug.gda.pl; 2 e-mail: czaprowska@wp.pl<br />

Lichens have been used in folk medicine for centuries. Many secondary metabolites<br />

have been identified and isolated from lichens and some of them were found to have<br />

antimicrobial activity. Although numerous studies were published on antibacterial activity of<br />

metabolites extracted from lichen thalli, mycelial cultures did not get that much attention so<br />

far. Antimicrobial activity of in vitro cultured mycelia of Protoparmeliopsis muralis (syn.<br />

Lecanora muralis) was tested against Gram-positive and Gram-negative bacteria. We tested<br />

acetone and methanol extracts from mycelia grown on PDA and GLBM media. The following<br />

bacterial strains were used in disc diffusion tests: Bacillus subtilis, Escherichia coli,<br />

Pseudomonas aeruginosa and Staphyllococcus aureus. We observed a stronger antibacterial<br />

activity against Gram-positive bacteria than Gram-negative strains.<br />

Acknowledgements. The study was financially supported by Marie Curie European<br />

Reintegration Grant within 7 th European Community Framework Programme project no.<br />

239343.<br />

<strong>LICHENS</strong> <strong>IN</strong> RED DATA BOOK OF LEN<strong>IN</strong>GRAD REGION – CURRENT<br />

SITUATION <strong>AND</strong> PERSPECTIVES<br />

D. E. HIMELBRANT, E. S. KUZNETSOVA,<br />

I. S. STEPANCHIKOVA<br />

Department of Botany, St. Petersburg State University, Universitetskaya Emb. 7/9, 199034 St.<br />

Petersburg, Russia<br />

Laboratory of Lichenology and Bryology, Komarov Botanical Institute of RAS, Prof. Popov<br />

Str. 2, 197376 St. Petersburg, Russia<br />

E-mails: d_brant@mail.ru, igel_kuzn@mail.ru, stepa_ir@mail.ru<br />

The first edition of Red Data Book (RDB) of Leningrad Region (LR) was published in<br />

2000. The list of lichens includes 49 species. At that moment it was about 7% of known<br />

lichen diversity in the region (Zavarzin et al., 1999). At present we have data about<br />

approximately 950 species inhabiting LR (e. g. Kuznetsova, 2007; Stepanchikova et al., 2009,<br />

2010). It means that only 5% of lichens are protected.<br />

The species were included in RDB according to the following criteria: 1) species listed<br />

in RDB of RSFSR (1988); 2) species that need protection throughout LR; 3) rare species

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